A5003293907- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Enzyme Catalysis and Immobilization
Massachusetts Institute of Technology
2025
University of California, Berkeley
2023
Yancheng Institute of Technology
2019
Using a fluorescence complementation assay, Delivered Complementation in Planta (DCIP), we demonstrate cell-penetrating peptide-mediated cytosolic delivery of peptides and recombinant proteins Nicotiana benthamiana. We show that DCIP enables quantitative measurement protein efficiency functional screening for in-planta delivery. Finally, detects recombinantly expressed such as mCherry Lifeact into intact leaves. also plant transcription factor, WUSCHEL (AtWUS), N. RT-qPCR analysis AtWUS...
Abstract Naturally existing enzymes have been adapted for a variety of molecular technologies, with enhancements or modifications to the introduced improve desired function; however, it is difficult engineer variants enhanced activity while maintaining specificity. Here we compact Obligate Mobile Element Guided Activity (OMEGA) RNA-guided endonuclease IscB and its guiding RNA (ωRNA) by combining ortholog screening, structure-guided protein domain design engineering, deep learning-based...
This study investigated the effects of different amino acids on fermentation pure inulin and glucose using Paenibacillus polymyxa ZJ-9 in order to improve production R,R-2,3-butanediol (R,R-2,3-BD) respectively. The extract from Jerusalem artichoke tubers contained 19 common acids, which were detected by HPLC. Arg featured highest content (1290 mg l-1 ). A single add-back experiment 20 indicated that Asn, Ser, His are key R,R-2,3-BD synthesis during P. ZJ-9. corresponding yields reached...