A5084311554- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advancements in Transdermal Drug Delivery
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Dermatology and Skin Diseases
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Surface Modification and Superhydrophobicity
- Advanced Sensor and Energy Harvesting Materials
- Ocular Surface and Contact Lens
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
University of Washington
2023-2025
Quanterix (United States)
2019-2023
Billerica Public Library
2020
We report approaches to improve the sensitivity of digital ELISA up 400-fold, enabling detection proteins at subattomolar concentrations.
We report methods that improve the quantification of digital bead assays (DBA)─such as enzyme-linked immunosorbent assay (ELISA)─that have found widespread use for high sensitivity measurement proteins in clinical research and diagnostics. In ELISA, are captured on beads, labeled with enzymes, individual beads interrogated activity from one or more average number enzymes per (AEB) is determined based Poisson statistics. The ELISA has revealed limitations to original approaches can lead...
We have developed a customizable contact printed multiplex immunoassay capable of simultaneously measuring up to five analytes with attomolar sensitivities. This enzyme-linked immunosorbent assay (ELISA) was based on spotting different antibodies in circular pattern at the bottom microtiter plate well. Unlike traditional antibody printing for ELISA that prints capture specific target interest, this we unique "anchor" well surface, each having high affinity peptide target. By coupling...
Electrostatic patterning has improved the performance of devices incorporating electrospun fibers in a wide variety applications. However, impact process parameters on final fiber pattern these systems is rarely analyzed. Herein, systematic analytical approach developed to define quantitative metrics related patterning. Three‐dimensional patterned dielectric elastomer collectors are fabricated via solution‐casting polydimethylsiloxane with embedded carbon black or liquid metal droplets....
Abstract Quantifying prospective biomarkers of immune signaling in biological samples requires high assay sensitivity. The Simoa Planar Array provides greater sensitivity than ELISA, but has a finite menu. This study aimed to develop universal multiplex ‘homebrew’ planar immunoassay format for development assays any analyte. Anchor antibodies specific peptide tag were microprinted microwell plates. tags conjugated analyte-specific capture via maleimide chemistry, enabling affinity binding...
Abstract The concentration of cytokines in blood are often at pictogram per mL and sub-picogram mL. Recent advances the sensitivity immunoassays, such as single molecule arrays (Simoa), have allowed detection many these that were previously undetectable blood. While limits (LOD) new technologies femtogram range, concentrations healthy individuals can still not be detected. In fact, quantitative measurement several important <50% individuals. We will report on to improve Simoa...