A5011161007- Carbohydrate Chemistry and Synthesis
- Various Chemistry Research Topics
- Chemical Synthesis and Analysis
- Organophosphorus compounds synthesis
- Glycosylation and Glycoproteins Research
- Analytical Chemistry and Chromatography
- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Phosphorus compounds and reactions
- RNA and protein synthesis mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Protein Structure and Dynamics
- Enzyme Catalysis and Immobilization
- Influenza Virus Research Studies
- Inorganic and Organometallic Chemistry
- Pigment Synthesis and Properties
- X-ray Diffraction in Crystallography
- Bone health and treatments
- Biological Research and Disease Studies
- Silicone and Siloxane Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Synthesis and Reactivity of Sulfur-Containing Compounds
University of Puget Sound
1992-2021
University of California, Berkeley
1989-2000
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHemagglutinins from two influenza virus variants bind to sialic acid derivatives with millimolar dissociation constants: a 500-MHz proton nuclear magnetic resonance studyNicholas K. Sauter, Mark D. Bednarski, Beth A. Wurzburg, John E. Hanson, George M. Whitesides, J. Skehel, and Don C. WileyCite this: Biochemistry 1989, 28, 21, 8388–8396Publication Date (Print):October 17, 1989Publication History Published online1 May 2002Published inissue 17...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBinding of influenza virus hemagglutinin to analogs its cell-surface receptor, sialic acid: analysis by proton nuclear magnetic resonance spectroscopy and x-ray crystallographyNicholas K. Sauter, John E. Hanson, Gary D. Glick, Jerry H. Brown, Robert L. Crowther, Seong Joon Park, J. Skehel, Don C. WileyCite this: Biochemistry 1992, 31, 40, 9609–9621Publication Date (Print):October 13, 1992Publication History Published online1 May 2002Published...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotent inhibition of pepsin and penicillopepsin by phosphorus-containing peptide analogsPaul A. Bartlett, John E. Hanson, Peter P. GiannousisCite this: J. Org. Chem. 1990, 55, 26, 6268–6274Publication Date (Print):December 1, 1990Publication History Published online1 May 2002Published inissue 1 December 1990https://pubs.acs.org/doi/10.1021/jo00313a012https://doi.org/10.1021/jo00313a012research-articleACS PublicationsRequest reuse permissionsArticle...
Analogues of tri- and tetrapeptide substrates carboxypeptidase A in which the scissile peptide linkage is replaced with a phosphonate moiety (-PO2--O-) were synthesized evaluated as inhibitors enzyme. The terminated either L-lactate or L-phenyllactate [designated (O) Ala Phe, respectively] P1' position. Transition-state analogy was shown for series 14 derivatives containing structure RCO-AlaP-(O)Ala [RCO-AP(O)A, AP indicates phosphonic acid analogue alanine] by correlation Ki values Km/kcat...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcetylenic Ethers. II. Ethoxy- and Butoxy-acetyleneThomas L. Jacobs, Richard Cramer, John E. HansonCite this: J. Am. Chem. Soc. 1942, 64, 2, 223–226Publication Date (Print):February 1, 1942Publication History Published online1 May 2002Published inissue 1 February 1942https://pubs.acs.org/doi/10.1021/ja01254a008https://doi.org/10.1021/ja01254a008research-articleACS PublicationsRequest reuse permissionsArticle Views402Altmetric-Citations38LEARN ABOUT...
Abstract Thioglycosides are hydrolase‐resistant mimics of O‐linked glycosides that can serve as valuable probes for studying the role in biological processes. The development an efficient, enzyme‐mediated synthesis thioglycosides, including S‐GlcNAcylated proteins, is reported, using a thioglycoligase derived from GH20 hexosaminidase Streptomyces plicatus which catalytic acid/base glutamate has been mutated to alanine (SpHex E314A). This robust, easily‐prepared, engineered enzyme uses GlcNAc...
In this expository paper aimed at a general mathematical audience, we discuss how to combine certain classic theorems of set-theoretic inner model theory and effective descriptive set with work on Hilbert's tenth problem universal Diophantine equations produce the following surprising result: There is specific polynomial $p(x,y,z,n,k_1,\dots,k_{70})$ degree $7$ integer coefficients such that it independent $\mathsf{ZFC}$ (and much stronger theories) whether function $$f(x) = \inf_{y \in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystallographic analysis of transition-state mimics bound to penicillopepsin: phosphorus-containing peptide analogsMarie E. Fraser, Natalie C. J. Strynadka, Paul A. Bartlett, John Hanson, and Michael N. G. JamesCite this: Biochemistry 1992, 31, 22, 5201–5214Publication Date (Print):June 9, 1992Publication History Published online1 May 2002Published inissue 9 June...
Peptidases play a variety of roles in living organisms, from the routine work digestion to highly specific hormonal regulation. They are involved such diverse processes as blood-clotting and complement cascades, blood pressure regulation, egg fertilization, posttranslational processing protein synthesis, tumor invasion, degenerative diseases emphysema arthritis. Synthetic naturally occurring peptidase inhibitors have been useful for studying function these enzymes medicinal agents. Indeed,...
Jacobsen's catalyst, N,N'-bis(3,5-di-tert-butylsalicylidene)-1,2-cyclohexanediaminomanganese(III) chloride, is a popular reagent for the enantioselective epoxidation of alkenes. This successfully prepared in three steps by beginning organic chemistry students. A mixture 1,2-diaminocyclohexane isomers purified and resolved crystallization (and recrystallization) with L-tartaric acid; diimine formed between trans-1,2-diaminocyclohexane 3,5-di-tert-butyl-2-hydroxybenzaldehyde to produce...
The refined crystal structure of the complex between human pepsin and a synthetic phosphonate inhibitor, Iva-Val-Val-LeuP-(O)Phe-Ala-Ala-OMe [Iva = isovaleryl, LeuP phosphinic acid analog l-leucine, (O)Phe l-3-phenyllactic acid, OMe methyl ester], is presented. was using diffraction data 30 1.96 Å resolution to final R factor (\textstyle \sum \big| | F_{o}| - |F_{c} \big / |F_{o} |, where |Fo| |Fc| are observed calculated structure-factor amplitudes, respectively) 20.0%. interactions...
Students in the second-semester organic chemistry laboratory perform a Wittig reaction between butylidenetriphenylphosphorane (an ylide) and benzaldehyde determine relative percentages of cis trans isomers 1-phenyl-1-pentene product. Because highly reactive nature this unstabilized ylide, students are introduced to working under anhydrous inert atmosphere conditions. prepare ylide by treatment butyltriphenylphosphonium bromide with THF solutions lithium, sodium, or potassium salt...
Abstract Thioglycosides are hydrolase‐resistant mimics of O‐linked glycosides that can serve as valuable probes for studying the role in biological processes. The development an efficient, enzyme‐mediated synthesis thioglycosides, including S‐GlcNAcylated proteins, is reported, using a thioglycoligase derived from GH20 hexosaminidase Streptomyces plicatus which catalytic acid/base glutamate has been mutated to alanine (SpHex E314A). This robust, easily‐prepared, engineered enzyme uses GlcNAc...
Screening of a human gut metagenomic library yielded set novel beta- N -acetyl-glucosaminidases. Mutation active site residues thioglycoligase that efficiently S -GlcNAcylates protein targets as well various thiosugar acceptors.