A5048535578- Phytase and its Applications
- Plant nutrient uptake and metabolism
- Pineapple and bromelain studies
- Plant Stress Responses and Tolerance
- Cellular transport and secretion
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Biomedical and Engineering Education
- Plant Reproductive Biology
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- GABA and Rice Research
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Plant tissue culture and regeneration
- Polyamine Metabolism and Applications
- Plant Physiology and Cultivation Studies
- Lipid metabolism and biosynthesis
- Biochemical and biochemical processes
- Pancreatic function and diabetes
- Microbial Metabolic Engineering and Bioproduction
University of Wisconsin–Madison
2024
Virginia Tech
2013-2023
Hebrew University of Jerusalem
2011
Salisbury University
2008
Biotechnology Research Center
2006
Scientific Research Institute of Biochemistry
2001
University of California, Berkeley
1993-1995
Case Western Reserve University
1991-1993
University School
1993
Embryonic development in many angiosperms occurs concomitantly with the of ovary into a specialized organ, fruit, which provides suitable environment for seed maturation and often mechanism dispersa1 mature seeds, as Darwin observed. Despite centuries intensive genetic selection agriculturally valuable we still lack most information about how fruits develop, this is coordinated embryonic formation, molecular, cellular, physiological events that control fruit growth differentiation. The last...
Abstract l-myo-inositol 1-phosphate synthase (MIPS; EC 5.5.1.4) catalyzes the rate-limiting step in synthesis of myo-inositol, a critical compound cell. Plants contain multiple MIPS genes, which encode highly similar enzymes. We characterized expression patterns three genes Arabidopsis thaliana and found that MIPS1 is expressed most cell types developmental stages, while MIPS2 MIPS3 are mainly restricted to vascular or related tissues. MIPS1, but not MIPS3, required for seed development,...
Abstract Myoinositol synthesis and catabolism are crucial in many multiceullar eukaryotes for the production of phosphatidylinositol signaling molecules, glycerophosphoinositide membrane anchors, cell wall pectic noncellulosic polysaccharides, several other molecules including ascorbate. monophosphatase (IMP) is a major enzyme required myoinositol breakdown (1,4,5)trisphosphate, potent second messenger involved biological activities. It has been shown that VTC4 from kiwifruit (Actinidia...
Inositol pyrophosphates are unique cellular signaling molecules with recently discovered roles in energy sensing and metabolism. Studies eukaryotes have revealed that these compounds a rapid turnover, thus only small amounts accumulate. not been the subject of investigation plants even though seeds produce large their precursor, myo-inositol hexakisphosphate (InsP6 ). Here, we report Arabidopsis maize InsP6 transporter mutants elevated levels inositol seed, providing unequivocal...
Abstract Plants possess remarkable ability to adapt adverse environmental conditions. The adaptation process involves the removal of many molecules from organelles, especially membranes, and replacing them with new ones. is mediated by an intracellular vesicle-trafficking system regulated phosphatidylinositol (PtdIns) kinases phosphatases. Although PtdIns comprise a fraction membrane lipids, they function as major regulators stress signaling. We analyzed role 5-phosphatases (5PTases) in...
myo-Inositol monophosphatase (IMP) is a soluble, Li(+)-sensitive protein that catalyzes the removal of phosphate from myo-inositol substrates. IMP required for de novo inositol synthesis glucose 6-phosphate and breakdown trisphosphate, second messenger generated by phosphatidylinositol signaling pathway. We cloned gene tomato (LeIMP) show plant enzyme encoded small family. Three different LeIMP cDNAs encode distinct but highly conserved enzymes are catalytically active in vitro. Similar to...
Abstract Signals can be perceived and amplified at the cell membrane by receptors coupled to production of a variety second messengers, including inositol 1,4,5-trisphosphate (IP3). We previously have identified 15 putative 5-phosphatases (5PTases) from Arabidopsis shown that At5PTase1 hydrolyze IP3. To determine whether terminate IP3-mediated signaling, we analyzed transgenic plants ectopically expressing At5PTase1. Stomata leaves were abscisic acid (ABA) light insensitive, ABA induction...
Abstract In plants, myoinositol signaling pathways have been associated with several stress, developmental, and physiological processes, but the regulation of these is largely unknown. our efforts to better understand in we found that WD40 repeat region a polyphosphate 5-phosphatase (5PTase13; At1g05630) interacts sucrose nonfermenting-1-related kinase (SnRK1.1) yeast two-hybrid system vitro. Plant SnRK1 proteins (also known as AKIN10/11) described central integrators sugar, metabolic,...
The Sucrose non-Fermenting Related Kinase 1 (SnRK1) proteins have been linked to regulation of energy and stress signaling in eukaryotes. In plants, there is a small SnRK1 gene family. While the SnRK1.1 has well studied, role other isoforms play or less understood. We used promoter:GUS analysis found broadly expressed, while SnRK1.2 spatially restricted. expressed most abundantly hydathodes, at base leaf primordia, vascular tissues within both shoots roots. examined impact that sugars on...
Plant aromatic amino acid decarboxylase (AAAD) enzymes are capable of catalyzing either decarboxylation or decarboxylation-deamination on various combinations substrates. These two different activities result in the production arylalkylamines and formation acetaldehydes, respectively. Variations product enable individual to play physiological functions. Despite these catalytic variations, arylalkylamine aldehyde synthesizing AAADs indistinguishable without protein expression...
Abstract The inositol triphosphate (IP3)-signaling pathway has been associated with several developmental and physiological processes in plants, but we currently know little about the regulation of this pathway. Inositol 5′ phosphatases (5PTases) are enzymes that remove a phosphate from potential second messengers, including IP3. In catalyzing removal messenger substrates, 5PTases can act to terminate signal transduction events. We describe molecular analysis At5PTase1, 5PTase gene...
Signals can be perceived and amplified at the cell membrane by receptors coupled to production of a variety second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise large protein family that hydrolyzes 5-phosphates from phosphate (InsP) phosphoinositide (PtdInsP) substrates. Arabidopsis thaliana has 15 genes encoding 5PTases. Biochemical analyses subgroup 5PTase enzymes suggest these have both overlapping...
Phospholipase C (PLC) is well known for its role in animal signaling, where it generates the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), by hydrolyzing minor phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2), upon receptor stimulation. In plants, PLC's still unclear, especially because primary targets of both messengers are lacking, i.e. ligand-gated Ca2+ channel protein kinase C, PIP2 levels extremely low. Nonetheless, Arabidopsis genome encodes...
ABSTRACT Fluorescent acid-fast microscopy (FAM) was used to enumerate intracellular Mycobacterium avium , intracellulare and scrofulaceum in the ciliated phagocytic protozoan Tetrahymena pyriformis . There a linear relationship between FAM colony counts of M. cells both from cultures within protozoa. The Ziehl-Neelsen stain could not be mycobacteria because uninfected protozoa contained acid-fast, bacterium-like particles. Starved, 7-day-old T. transferred into fresh medium readily...
Abstract Phosphoinositides are important molecules that serve as second messengers and bind to a complex array of proteins modulating their subcellular location activity. The enzymes metabolize phosphoinositides can in some cases terminate the signaling actions phosphoinositides. inositol polyphosphate 5-phosphatases (5PTases) comprise large protein family hydrolyzes 5-phosphates from variety phosphate phosphoinositide substrates. We previously reported identification 15 putative 5PTase...
myo-Inositol is a precursor for cell wall components, used as backbone of myo-inositol trisphosphate (Ins(1,4,5)P(3)) and phosphatidylinositol phosphate signaling molecules, debated about whether it also in an alternate ascorbic acid synthesis pathway. Plants control inositol homeostasis by regulation key enzymes involved catabolism. Recent transcriptional profiling data indicate up-regulation the oxygenase (MIOX) genes under conditions which energy or nutrients are limited. To test MIOX...
ABSTRACT Inositol pyrophosphates (PP-InsPs) are an emerging class of “high-energy” intracellular signaling molecules containing one or two diphosphate groups attached to inositol ring, with suggested roles in bioenergetic homeostasis and inorganic phosphate (P i ) sensing. Information regarding the biosynthesis these unique plants is scarce, however enzymes responsible for their other eukaryotes have been well described. Here we report characterization Arabidopsis VIP kinase domains, a newly...
Synthesis of myo-inositol is crucial in multicellular eukaryotes for production phosphatidylinositol and inositol phosphate signaling molecules. The monophosphatase (IMP) enzyme required the synthesis myo-inositol, breakdown (1,4,5)-trisphosphate, a second messenger involved Ca(2+) signaling, L-galactose, precursor ascorbic acid. Two -like (IMPL) genes Arabidopsis encode chloroplast proteins with homology to prokaryotic IMPs one these, IMPL2, can complement bacterial histidinol 1-phosphate...