The present work was carried out to study the effects of gamma irradiation on in vitro growth explants, callus and formation shoots plantlets. Irradiation is known exhibit or inhibit differentiation cells plants vitro, which helps producing new plant varieties. Gamma one physical mutagens that are widely used for mutation breeding. A gradual decline observed number regenerated from irradiated petiole explants compared control. Numbers explant cultured Murashige & Skoog medium...

Artificial seeds were produced from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African violet). The production artificial this species gave ideal beads based on firmness, texture, size and shape beads. percentage germination influenced by the concentrations sodium alginate calcium chloride (CaCl2.2H2O) used. It was found that among tested, 3 exposure to 1.00 mM CaCl2.2H2O solution for 30 min had optimal with firm, clear, round uniform suitable handling. also observed obtained...

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations 0.5 mg/L BAP and 1.5 NAA which resulted in 16.00 ± 0.30 number shoots per explant exhibited new characteristic layered multiple shoots, while normal roots formed the basal media. The double-layered gave highest ratio 2 : 1 (liquid to solid) mean 16.67 0.23 formation primary secondary explants. study involving...

Direct somatic embryogenesis induction of Begonia x hiemalis Fotsch. (Elatior Begonia) was initiated from two different explants i.e., leaves and petioles. Both were cultured on MS medium supplemented with concentrations Benzylaminopurine (BAP) 2,4-Dichlorophenoxyacetic acid (2,4-D). The results showed that combinations 0.5-1.0 mg L-1 BAP 0.1 2,4-D produced direct leaf petiole explants. Different casein hydrolysate also tested to optimize embryo induction. 100 could produce 53.08 nodular...

In the present study, in vitro mutagenesis techniques were applied to investigate effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes callus Gerbera jamesonii Bolus ex. Hook f. Biochemical chlorophyll soluble protein content pre- post- irradiated studied. Non-irradiated demonstrated highest amount as compared Gy. addition, b was relatively higher than a both non-irradiated callus, except for 10 differentiation based total revealed gradual reduction after...

Cell suspension cultures were established from embryogenic callus induced leaf explants of Gerbera jamesonii Bolus ex. Hook f. Embryogenic was when cultured on MS medium containing 1.0 to 2.0 mg/L 2,4-D. Cream friable formed within two weeks. Proliferated transferred liquid 2,4-D with a small concentration NAA and subcultured at 2 weeks interval. Induction somatic embryos (globular, heart torpedo) observed after culture. Somatic developed in 0.1 or globular further differentiated into the...

Oil palm (Elaeis guineensis) planting is one of the economic pillars countries rich in biodiversity tropical regions. However, pest and disease are most serious limitation oil production. Bagworm, Metisa plana (Lepidoptera: Psychidae) considered destructive plantation Malaysia, which could cause high yield losses up to 43% over two years after a infestation become primary concern among stakeholders. Conventional excessive use pesticides causes environmental pollutions damage non-target...

Efficient plant regeneration of Saintpaulia ionantha (African violet) has been obtained in the present study. MS medium supplemented with 1.0 mg L(-1) IAA and 2.0 Zeatin resulted 100% shoot induced highest number shoots (average 15.0 +/- 0.8 per explant) after being cultured for 8 weeks. The above hormone combination was optimum regeneration. Most plantlets derived from tissue culture system could be hardened transferred to greenhouse conditions 84.0 1.6% success rate. However, regenerated...

Plant tissues such as somatic embryos, apical shoot tips, axillary buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used explant sources obtaining Microshoots induced from stem explants on Murashige Skoog (MS) medium supplemented with 1.5 mg/L benzylaminopurine (BAP). They encapsulated in 3% (w/v) sodium alginate, sucrose, 0.1 BAP,...

Adventitious shoots of Gerbera jamesonii Bolus ex. Hook f. were successfully obtained when petiole explants from aseptic seedlings cultured on MS media supplemented with 2.0 mg/L 6-Benzylaminopurine (BAP) and 0.5 α-Naphthalene acetic acid (NAA). The subcultured every 2 weeks the same culture to obtain in vitro plantlets. Mature plantlets achieved after 8 subculture. These transferred Basal for further root elongation weeks. Complete several growth acclimatization process. substrates garden...

In vitro flowering of three selected ornamental species from Malaysia was studied. Oxalis triangularis, Celosia cristata and Begonia x hiemalis were found to be able flower on MS Murashige Skoog, 1962) medium supplemented with various hormones, such as Benzyl aminopurine (BAP), Naphthalene acetic acid ( NAA), etc at different concentrations. tissue culture, in is normally induced for many reasons. One the most important ones being shorten life cycles plants, other aims include gather...

Saintpaulia ionantha H. Wendl or commonly known as African violet was successfully established in tissue culture system. Complete plant regeneration achieved from leaf, petiole, peduncle and floral parts of the plant. MS medium supplemented with 1.0 mg/L IAA 2.0 zeatin found to be best for shoot present work. The plantlets were acclimatized managed survive green house. method acclimatization this species obtained when vitro transferred into planting pots containing mixed soil, which is a...

The National Service Training Programme better known as Program Latihan Khidmat Negara (PLKN) was launched by the Malaysian government on 16th of February 2004 to foster national unity and integration. However, since its inception, no specific empirical study has been conducted physical activity module (adventure-based activity) measure effectiveness programme in improving process Modified Group Environment Questionnaire (GEQ) used collect required data which administered during pre...

Comparative anatomical studies were carried out on in vivo plants of Triphasia trifolia (Burm.f.) P. Wilson and vitro plantlets the same age.To get plantlets, explants cultured MS (Murashige Skoog) media supplemented with different concentrations combinations hormones.The explant sources obtained from cotyledons, leaves, stems, roots shoot tips that placed under conditions 16 hours light 8 dark.The optimum for regeneration was 1.0 mg/L BAP NAA.Cotyledon found to be most...

In vitro shoots without roots from different media compositions were selected and transferred into test tubes supplemented with MS medium hormone for root induction. After 4 weeks, full grown plantlets to sterile soil acclimatization process. The maintained in the culture room at 25±1°C 16 hours light 8 dark. Plantlets then planted pots covered polystyrene plastic bags. They grew well after weeks room, healthy greenhouse. Survival rate of acclimatized was 70% observation it showed that most...

Gerbera jamesonii (Asteraceae) is a flowering plant with increasing commercial significance. Today, this has become one of the leading cut flowers and ranks among top 10 in world. It known to contain high percentage anthocyanin. petals can be good colourant source and, potentially, antioxidants. Having potential for producing colourants, focus research thus mainly aimed extract anthocyanin pigments from fresh G. study optimum condition natural paint production extracts. Petals were collected...

Studies of Fortunella polyandra 'Nagami' and 'Meiwa' were carried out to observe their responses in tissue culture systems.Explant sources investigated included roots, stems, leaves cotyledons which cultured on Murashige Skoog (MS) medium supplemented with different combinations concentrations various hormones.The hormones used naphthalene acetic acid (NAA) benzylaminopurine (BAP) at pH 5.8 a temperature 23-26°C photoperiod 16 hours light 8 dark.The the media was also altered include 4.8,...

In this study, a wild relative of Citrus, Severinia buxifolia, was subjected to tissue culture treatments in order identify the best medium for plant regeneration species.This research carried out due its range uses and because potential be rootstock species that is tolerant boron.Different sources explants were cultured on Murashige Skoog (MS) media supplemented with various types concentrations hormones.Results indicated responsive from stems cotyledons, based formation shoots.The shoot...

Somatic embryogenesis is a multi-step process for the induction of somatic embryogenesis, embryo development, maturation and germination. All plantlets obtained through did not differ phenotypically from parental clones (Stefaniak, 1994). In this study, several plants such as Gerbera jamesonii, Begonia x hiemalis Fotsch., Saintpaulia ionantha were successfully regenerated process. Explants leaf, petiole stem used to induce embryos all plant species. Explant cultured on Murashige Skoog (MS)...