ABSTRACT There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to standardized protocol requires high degree confidence data across laboratories. Data typically determined through series specifications that ensure good experimental practice and absence bias results due DNA isolation amplification interferences. However, there currently lack...

Plasmid sequences are central to a myriad of microbial functions and processes. Here, we have compiled database complete plasmid associated metadata curated from both NCBI's recent genome update, which includes plasmids as organisms, all available annotated bacterial genomes. The resultant contains 10,892 metadata.

Increasing rates of antibiotic-resistant bacterial infection are one the most pressing contemporary global health concerns. The ESKAPE pathogen group represents leading cause these infections, and upregulation efflux pump expression is a significant mechanism resistance in pathogens. This has resulted substantial interest development inhibitors to combat infections; however, no widespread treatments have been developed date. Our study evaluates an often-underappreciated aspect resistance—the...

Children are increasingly being diagnosed with primary hypertension. The absence of comparative effectiveness research antihypertensive medications in children has contributed to considerable differences prescribing practices among physicians treating Even if parallel-group trials had established a best overall choice for most these children, the medication an individual may differ from medication. This project consists series systematically administered n-of-1 older verify need ongoing...

Describing the role of plasmids and their contribution to exchange genetic material among bacteria is essential for understanding fields plasmid epidemiology, microbial ecology, commercial synthetic microbiology. Broad-host-range (BHR) are those that found not only in a single bacterial species, but members different taxonomic groups significant interest researchers many fields. We applied novel approach computationally identify new BHR plasmids, which we searched highly similar cognate...

The crisis of antimicrobial resistant bacterial infections is one the most pressing public health issues. Common agricultural practices have been implicated in generation bacteria. Biopesticides, live bacteria used for pest control, are non-pathogenic and considered safe consumption. Application bacteria-based pesticides to crops high concentrations raises possibility unintentional contributions movement resistance genes environment. However, presence clinically relevant their phenotypes...

To compare decay profiles of ruminant‐ and cattle‐associated molecular markers for faecal contamination Escherichia coli, facilitating their correct application in water quality studies. We generated cultivable E. a general Bacteroidales genetic marker (GenBac3), ruminant (CF128, Rum2Bac) cattle (CowM2, CowM3) using faeces‐seeded mesocosms, selected best fitting models each profile. Global model tested differences between profiles. After normalizing initial concentration, curves differed...

We present a case of 17-year-old Hispanic male with Arnold-Chiari Type 1 [AC-Type 1] syringomyelia, status post decompression, who complains exercise intolerance, headaches, and fatigue exertion. The patient was found to have diurnal hypercapnia nocturnal alveolar hypoventilation. Cardiopulmonary testing revealed blunting the ventilatory response rise in carbon dioxide (CO 2 ) resulting failure parallel correlation between increased CO levels ventilation; expected vertical relationship PETCO...

Antibiotic resistance is one of the largest threats facing global health. Wastewater treatment plants are well-known hot spots for interaction between diverse bacteria, genetic exchange, and antibiotic resistance. Nonpathogenic bacteria theoretically act as reservoirs subsequently transferring genes to pathogens, indicating that evolutionary processes occur outside clinical settings may drive patterns drug-resistant infections. We isolated sequenced 100 bacterial strains from five wastewater...

Abstract The crisis of antibiotic resistant bacterial infections is one the most pressing public health issues. Common agricultural practices have been implicated in generation bacteria. Biopesticides, live bacteria used for pest control, are non-pathogenic and considered safe consumption. Application bacteria-based pesticides to crops high concentrations raises possibility unintentional contributions movement resistance genes environment. However, presence clinically relevant their...