A5063839540- Antibiotic Resistance in Bacteria
- Statistical Distribution Estimation and Applications
- Immunotherapy and Immune Responses
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Probabilistic and Robust Engineering Design
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Fuzzy Systems and Optimization
- Respiratory viral infections research
- Helicobacter pylori-related gastroenterology studies
- Influenza Virus Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Poxvirus research and outbreaks
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Pneumonia and Respiratory Infections
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
- Galectins and Cancer Biology
- Advanced Statistical Methods and Models
Brock University
2015-2025
National Research Council Canada
2016-2025
Jishou University
2017-2024
Huazhong Agricultural University
2024
First People's Hospital of Nanning
2012-2022
Health Canada
2019
National University of Singapore
2016
National Academies of Sciences, Engineering, and Medicine
2003-2015
Central China Normal University
2013-2014
Institute for Biological Sciences
2003-2012
Acinetobacter baumannii has emerged as a major cause of both community-associated and nosocomial pneumonia, but little is known about the cellular molecular mechanisms host defense against respiratory infection with this bacterial pathogen. In study, we examined role neutrophils in resistance to pulmonary A. mouse model intranasal (i.n.) infection. We found that were rapidly recruited lungs following i.n. inoculation pathogen declined baseline level upon clearance Depletion using monoclonal...
Generation of protective immunity at mucosal surfaces can greatly assist the host defense against pathogens which either cause disease epithelial barriers or enter through these surfaces. Although routes immunization, such as intranasal and oral, are being intensely explored appear promising for eliciting in mammals, their application clinical practice has been limited due to technical safety related challenges. Most currently approved human vaccines administered via systemic (such...
ABSTRACT Acinetobacter baumannii is an important emerging pathogen in health care-acquired infections and responsible for severe nosocomial community-acquired pneumonia. Currently available mouse models of A. pneumonia show poor colonization with little to no extrapulmonary dissemination. Here, we describe a model using clinical isolate (LAC-4 strain) that reliably reproduces the most relevant features human pulmonary infection pathology. Using this model, have shown LAC-4 induced rapid...
Acinetobacter baumannii is an emerging bacterial pathogen that causes nosocomial pneumonia and other infections. Although it recognized as increasing threat to immunocompromised patients, the mechanism of host defense against A. infection remains poorly understood. In this study, we examined potential role macrophages in using vitro macrophage culture mouse model intranasal (i.n.) infection. Large numbers were taken up by alveolar vivo early 4 h after i.n. inoculation. By 24 h, induced...
We show in this study that toxin production Clostridium difficile is altered cells which can no longer form flagellar filaments. The impact of inactivation fliC, CD0240, fliF, fliG, fliM, and flhB-fliR genes upon levels culture supernatants was assessed using cell-based cytotoxicity assay, proteomics, immunoassay, immunoblotting approaches. Each these showed were significantly increased a fliC mutant compared to the C. 630 parent strain. In contrast, secreted from other mutants reduced with...
Acinetobacter baumannii is an important human pathogen due to its multi-drug resistance. In this study, the genome of ST10 outbreak A. isolate LAC-4 was completely sequenced better understand epidemiology, antibiotic resistance genetic determinants and potential virulence factors. Compared with 20 other complete genomes baumannii, harbors at least 12 copies five distinct insertion sequences. It contains 14 two novel IS elements, ISAba25 ISAba26, respectively. Additionally, three composite...
Acinetobacter baumannii is an important nosocomial pathogen. Mechanisms that allow A. to cause human infection are still poorly understood. Iron essential nutrient for bacterial growth in vivo, and the multiplicity of iron uptake systems suggests acquisition contributes ability infection. In Gram-negative bacteria, active transport ferrisiderophores heme relies on conserved TonB-ExbB-ExbD energy-transducing complex, while ferrous mediated by Feo system. The genome invariably contains three...
Pseudomonas aeruginosa is associated with both community and hospital-acquired infections. It colonizes the lungs of cystic fibrosis (CF) patients, establishing an ecological niche where it adapts evolves from early to chronic stages, resulting in deteriorating lung function frequent exacerbations. With antibiotics resistance on rise, there a pressing need for alternative personalized treatments (such as bacteriophage therapy) combat P. In this study, we aimed isolate characterize phages...
Francisella tularensis subsp. (type A) strain SCHU S4 is a prototypic of the pathogen that highly virulent for humans and other mammals. Its intradermal (i.d.) 50% lethal dose (LD50) mice <10 CFU. We discovered spontaneous mutant, designated FSC043, with an i.d. LD50 >10(8) FSC043 effectively vaccinated against challenge type A strain, protective efficacy was at least as good F. LVS, empirically attenuated which has been used efficacious human vaccine. Comparative proteomics to identify two...
Neutralization of carboxylic acid is an important means to avoid sialic dissociation when sialylated glycans are analyzed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). In this paper, we describe a simple and rapid method modify the acids in presence methylamine (7-azabenzotriazol-1-yloxy) trispyrrolidinophosphonium hexafluorophosphate (PyAOP). After methylamidation, can be MALDI-MS without loss moiety. The electrospray (ESI-MS) analysis both 3′-...
ABSTRACT We investigated the ability of compounds interfering with iron metabolism to inhibit growth Acinetobacter baumannii . Iron restriction transferrin or 2,2-bipyridyl significantly inhibited A. in vitro Gallium nitrate alone was moderately effective at reducing but became bacteriostatic presence serum transferrin. More importantly, gallium treatment reduced lung bacterial burdens mice. The use gallium-based therapies shows promise for control multidrug-resistant
The huge worldwide demand for vaccines targeting SARS-CoV-2 has necessitated the continued development of novel improved formulations capable reducing burden COVID-19 pandemic. Herein, we evaluated protein subunit vaccine containing a resistin-trimerized spike antigen, SmT1. When combined with sulfated lactosyl archaeol (SLA) archaeosome adjuvant, induced robust antigen-specific humoral and cellular immune responses in mice. Antibodies had strong neutralizing activity, preventing viral...
Introduction The incidence of Lyme disease (LD) in Canada and the United States has risen over last decade, nearing 480,000 cases each year. Borrelia burgdorferi sensu lato, causative agent LD, is transmitted to humans through bite an infected tick, resulting flu-like symptoms often a characteristic bull’s-eye rash. In more severe cases, disseminated bacterial infection can cause arthritis, carditis neurological impairments. Currently, no vaccine available for prevention LD humans. Methods...
Natural regulatory T cells (Tregs) constitutively express the IL-2R alpha-chain (CD25) on their surface. Consequently, administration of anti-CD25 Abs is a commonly used technique to deplete Treg populations in vivo. However, activated effector may also transiently CD25, and are thus potential targets for Abs. In this study using Toxoplasma gondii as model proinflammatory infection, we have examined capacity target cell during an inflammatory episode, determine what extent that action...
Francisella tularensis, which is a Gram negative bacterium that causes tularemia, has been classified by the Center for Disease Control and Prevention (CDC) as category A bioweapon. The development of vaccines, immunotherapeutics, diagnostics F. tularensis requires detailed knowledge saccharide structures can be recognized protective antibodies. We have synthesized inner core region lipopolysaccharide (LPS) to probe antigenic responses elicited live subunit vaccine. successful preparation...
Acinetobacter baumannii is a major cause of healthcare-associated infections worldwide with high morbidity and mortality. The clinical treatment A. has become increasingly difficult because the rapid emerging multidrug extremely drug resistant strains. Thus, there an urgent need for development novel intervention strategies to combat this multidrug-resistant pathogen. Vaccine one most effective medical measures infection control likely overcome resistance by baumannii. Here we discussed...