A5060267456- Hemoglobin structure and function
- Streptococcal Infections and Treatments
- Carbohydrate Chemistry and Synthesis
- Protein Structure and Dynamics
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Heme Oxygenase-1 and Carbon Monoxide
- Diet and metabolism studies
- Bacterial Identification and Susceptibility Testing
- Digestive system and related health
- Pneumonia and Respiratory Infections
- Enzyme Structure and Function
- Neonatal and Maternal Infections
- Toxin Mechanisms and Immunotoxins
- Birth, Development, and Health
- Phytochemistry and Biological Activities
- Antimicrobial Resistance in Staphylococcus
- Phytochemistry and biological activities of Ficus species
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Food Allergy and Anaphylaxis Research
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
Xinjiang Technical Institute of Physics & Chemistry
2025
University of Chinese Academy of Sciences
2025
United States Military Academy
2024
Jilin Normal University
2005-2022
Merck & Co., Inc., Rahway, NJ, USA (United States)
2022
University of Notre Dame
2015-2020
Nankai University
2008-2018
Ningbo University
2018
University of Michigan–Dearborn
2017
W. M. Keck Foundation
2017
Many important proteins perform their physiological functions under allosteric control, whereby the binding of a ligand at specific site influences affinity different site. Allosteric regulation usually involves switch in protein conformation upon binding. The energies corresponding structures are comparable, and, therefore, possibility that structure determined by x-ray diffraction crystalline state is influenced its intermolecular contacts, and thus differs from solution structure, cannot...
Sodium-ion hybrid capacitors (NHCs) have been attracting research interest in recent years. However, NHCs suffer from slower redox reaction kinetics of electrodes as compared to non-Faradaic capacitive counterparts. Herein, a high-performance NHC using porous NaBi anode, activated carbon (AC) cathode, and 1.5 M NaPF6 diglyme electrolyte is reported. In charging process, Na+ inserted into form Na3Bi, PF6– stored the electric double layers AC cathode; reverse Na3Bi desodiated eventually Bi,...
UDP-galactopyranose mutase (UGM) is the key enzyme involved in biosynthesis of Galf. UDP-Galp and UDP-Galf are two natural substrates UGM. A protocol that combines use STD-NMR spectroscopy, molecular modeling, CORCEMA-ST calculations was applied to investigation binding its C3-fluorinated analogue UGM from Klebsiella pneumoniae. UDP-[3-F]Galf were bound a manner similar UDP-Galp. The interconversions their galactopyranose counterparts catalyzed by reduced (active) with different catalytic...
A new multidentate ligand 1-(9-(1H-1,2,4-triazol-1-yl)anthracen-10-yl)-1H-1,2,4-triazole (tatrz) was designed and synthesized. Using tatrz as a building block, three novel coordination frameworks, namely, {[Cu(tatrz)2(NO3)2]·(CH3OH)·4H2O}n (1), {[Cu(tatrz)2(H2O)2](BF4)2}n (2), [Mn(tatrz)2(SCN)2(CH3OH)]·2H2O (3) can be isolated. Anion-exchange experiment indicates that NO3– anions in the two-dimensional (2D) copper framework of 1 completely exchanged by ClO4– an irreversible single...
It is generally accepted that the conversion of substrate should be kept at less than 10% total used when studying enzyme kinetics. However, or often will not produce sufficient signal changes required for robust high-throughput screening (HTS). To increase signal-to-background ratio, HTS performed higher conversion. Because consequences high are poorly understood, results sometimes questioned by enzymologists. The quality an assay judged ability to detect inhibitor under conditions, which...
The syntheses of N-alkylated deoxynojirimycin and 1,5-dideoxy-1,5-iminoxylitol derivatives having either a d- or an l-erythritol-3-sulfate functionalized N-substituent are reported. alkylating agent used was cyclic sulfate derivative, whereby selective attack the nitrogen atom at least hindered primary center afforded desired ammonium salt. In aqueous solution, these salts were configurationally labile center. Sulfonium and/or selenonium analogues prepared by analogous reactions. chalcogen...
A combined STD-NMR/molecular modeling protocol to probe the binding modes of glycosidase inhibitors kifunensine and salacinol Drosophila melanogaster Golgi α-mannosidase II (dGMII) was tested. Saturation-transfer difference (STD) NMR experiments were carried out for complexes dGMII with these two inhibitors. The program AutoDock 3.0 then used optimize interactions residues in active site dGMII. Theoretical STD effects ligand protons calculated different recently developed CORCEMA-ST...
UDP-galactopyranose mutase (UGM) is the key enzyme involved in biosynthesis of Galf. In this study, reliable structural binding modes natural substrate, UDP-Galp, and inhibitor, UDP, UGM active site were provided with combined use STD-NMR spectroscopy, molecular modeling, CORCEMA-ST calculations. UDP-Galp UDP exhibited similar epitopes recognized by UGM. However, relative affinities ligands changed dramatically upon reduction UGM, as explored competitive experiments. competes for to...
Heart and red blood cell endogenous antioxidant status plasma lipids were investigated in hypertensive, 14-week-old spontaneously hypertensive rats (SHR) normotensive Wistar-Kyoto (WKY) fed a standard commercial rat chow. Specific heart enzyme activities, as well the susceptibility of tissues to H2O2-induced glutathione (GSH) depletion lipid peroxidation, measured. Systolic pressure SHR was greater than WKY at 13 weeks age (197 +/- 12 vs. 132 14 mmHg (1 = 133.3 Pa); p < or 0.05), confirming...
Multivalent pneumococcal vaccines have been developed successfully to combat invasive diseases (IPD) and reduce the associated healthcare burden. These employ capsular polysaccharides (PnPs), either conjugated or unconjugated, as antigens provide serotype-specific protection. Pneumococcal used for vaccine often contain residual levels of cell wall (C-Ps), which can generate a non-serotype specific immune response complicate desired immunity. Therefore, C-P level in needs be controlled...
Plasma and lipoprotein lipid composition endogenous hepatic antioxidant status were investigated in hypertensive, 14-week-old spontaneously hypertensive (SHR) normotensive Wistar-Kyoto (WKY) rats fed a standard commercial rat chow. Total plasma calcium magnesium concentrations similar between both strains; however, systolic blood pressure SHR was greater than WKY at 13 weeks of age (197 +/- 12 vs. 132 14 mmHg; p < or = 0.05), confirming hypertension SHR. cholesterol triacylglycerol lower (p...
Model-free-based NMR dynamics studies have been undertaken for polypeptide backbone amide N−H bond vectors both the deoxy and carbonmonoxy forms of chain-specific, isotopically (15N 2H) labeled tetrameric hemoglobin (Hb) using 15N-relaxation parameters [longitudinal relaxation rate (R1), transverse (R2), heteronuclear nuclear Overhauser effect (NOE)] measured at two temperatures (29 34 °C) magnetic field strengths (11.7 14.1 T). In human normal adult (Hb A), bonds most amino acid residues...
Abstract UDP‐Galactopyranose mutase (UGM) is a flavoenzyme that catalyzes interconversion of UDP‐galactopyranose (UDP‐Galp) and UDP‐galactofuranose (UDP‐Galf); its activity depends on FAD redox state. The enzyme vital to many pathogens, not native mammals, an important drug target. We have probed binding substrate, UDP‐Galp, UDP wild type W160A UGM from K. pneumoniae, propose substrate directs recognition loop dynamics by bridging distal W160 sites; interacts with uracil the functionally...
The binding of human plasminogen (hPg) to the surface pathogen group A
On the basis of X-ray crystal structures and electron paramagnetic resonance (EPR) measurements, it has been inferred that O(2) binding to hemoglobin is stabilized by hydrogen bonds between oxygen ligands distal histidines. Our previous study multinuclear nuclear magnetic (NMR) spectroscopy provided first direct evidence such H-bonds in human normal adult oxyhemoglobin (HbO(2) A) solution. Here, NMR spectra uniformly (15)N-labeled recombinant Hb A (rHb five mutant rHbs oxy form have studied...
This study is aimed at investigating the molecular basis of environmental adaptation woolly mammoth hemoglobin (Hb) to harsh thermal conditions Pleistocene ice ages. To this end, we have carried out a comparative biochemical–biophysical characterization structural and functional properties recombinant hemoglobins (rHb) from (rHb WM) Asian elephant AE) in relation human Hb A A2 (a minor component blood). We obtained oxygen equilibrium curves calculated O2 affinities, Bohr effects, apparent...