A5101808982- Sympathectomy and Hyperhidrosis Treatments
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Cardiac Arrhythmias and Treatments
- Atrial Fibrillation Management and Outcomes
- Glycogen Storage Diseases and Myoclonus
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- Pleural and Pulmonary Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac and Coronary Surgery Techniques
- Cardiac electrophysiology and arrhythmias
- Lung Cancer Diagnosis and Treatment
- Mechanical Circulatory Support Devices
- Aortic Disease and Treatment Approaches
- Angiogenesis and VEGF in Cancer
- Urticaria and Related Conditions
- Systemic Sclerosis and Related Diseases
- Myasthenia Gravis and Thymoma
- Surgical Simulation and Training
- Streptococcal Infections and Treatments
- HER2/EGFR in Cancer Research
- Reconstructive Surgery and Microvascular Techniques
- Erythrocyte Function and Pathophysiology
University Medical Center Groningen
2014-2023
University of Groningen
2014-2023
Martini Ziekenhuis
2022
Ziekenhuis Groep Twente
2016
Luigi Sacco Hospital
2012
University of Milan
1971-2012
Ospedale Valduce
2009
University of Pisa
1993-2009
Novartis (Switzerland)
2008
Instituto Nazionale Tumori Regina Elena
1992
Background. We previously reported that group A Streptococcus (GAS) pili are the T antigens described by Rebecca Lancefield. also showed these pili, constituted backbone, ancillary 1, and 2 proteins, confer protection against GAS challenge in a mouse model.
BACKGROUND AND OBJECTIVES The diagnosis of pulmonary nodules unknown origin is challenging, and such are not always suitable for transthoracic needle biopsy. With the advent video assisted thoracic surgery (VATS) CT‐guided percutaneous hookwire localization (CT‐PHL) we hypothesized that combination these two procedures will improve early diagnosis. METHODS Selection criteria were a nodule well approachable with fine biopsy therapeutic consequences as assessed by multidisciplinary oncology...
Papillary muscle rupture (PMR) is a rare, but dramatic mechanical complication of myocardial infarction (MI), which can lead to rapid clinical deterioration and death. Immediate surgical intervention considered the optimal most rational treatment, despite high risks. In this study we sought identify overall long-term survival its predictors for patients who underwent mitral valve surgery post-MI PMR. Fifty consecutive (mean age 64.7 ± 10.8 years) repair (n = 10) or replacement 40) PMR from...
Primary palmar and/or axillary focal hyperhidrosis is a frequent disorder characterized by excessive sweating beyond physiological needs, often leading to substantial impairment of quality life. Over the years several minimally invasive surgical treatments have been described, however results vary, and due lack uniform approach, technique nomenclature are difficult compare. In this prospective study we sought evaluate safety effectiveness our standardized single-port, one-stage bilateral...
The age-related and T cell-independent immunological properties of most capsular polysaccharides limit their use as vaccines, especially in children under 2 years age. To overcome these limitations, polysaccharide antigens have been successfully conjugated to a variety carrier proteins, such diphtheria toxoid or tetanus (TT) the mutant (CRM197) produce very successful glycoconjugate vaccines. increasing demand for new conjugate vaccines requires availability additional carriers providing...
Abstract We isolated an IgG 2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi‐drug‐resistant (MDR) human cells. Its specificity toward the MDR I gene product (P‐glycoprotein) has been demonstrated by concordant segregation of MAb57 epitope in interspecific mouse × cell hybrids, and reactivity several different gene‐expressing cells Mab57, particularly insect acutely infected a baculovirus encoding gene. Mab57 can be used to detect, flow cytometry, variations...
Streptococcal pullulanases have been recently proposed as key components of the metabolic machinery involved in bacterial adaptation to host niches. By sequence analysis Group B Streptococcus (GBS) genome we found a novel putative surface exposed protein with pullulanase activity. We named such SAP. The sap gene is highly conserved among GBS strains and homologous genes, PulA SpuA, described other pathogenic streptococci. SAP contains two N-terminal carbohydrate-binding motifs, followed by...
Combined 'hybrid' thoracoscopic and percutaneous atrial fibrillation (AF) ablation is a strategy used to treat AF in patients with therapy-resistant symptomatic AF. We aimed study efficacy safety of single-stage hybrid persistent AF, or paroxysmal failed endocardial ablation, assess determinants success quality life. included consecutive undergoing ablation. First, we performed epicardial via access, isolate the pulmonary veins superior caval vein create posterior left box. Thereafter,...
The oncogene HER-2/neu encodes a transmembrane glycoprotein of 185 kDa (gp185HER-2) with tyrosine-kinase activity. Gene amplification and high levels expression gp185HER-2 have been found to correlate poor clinical outcome in breast ovarian carcinomas. Employing somatic cell hybrid fusion protocol, which yields frequency production hybridomas, we analyzed the extent murine immune response gp extracellular domain. In single experiment, using as immunogen NIH 3T3 cells expressing transfected...
Introduction The rising prevalence of modifiable risk factors (eg, obesity, hypertension and physical inactivity) is causing an increase in possible avoidable complications patients undergoing cardiac surgery. This study aims to assess whether a combined preoperative postoperative multidisciplinary rehabilitation (CR) programme (Heart-ROCQ programme) can improve functional status reduce surgical complications, readmissions major adverse events (MACE) as compared with standard care. Methods...
ABSTRACT A competitive ELISA method is described for the measurement of total antibodies to capsular polysaccharide Haemophilus influenzae type b (HibCPS) in human sera. The showed an excellent correlation radioantigen binding assay (RABA, or Farr assay) and improved sera with low titers respect more conventional noncompetitive method. Overestimation samples concentration range was no longer observed free HibCPS competition allowed us eliminate day-to-day background variation typical some...
Abstract We describe a murine IgG, monoclonal antibody (MAb56), specific for cell‐surface protein structure (MC56 determinant) expressed by the human CEM cell line. A large band of approximately 90 kDa was identified as main component MC56 determinant. Such 90‐kDa is significantly associated with drug‐sensitive phenotype, its expression being progressively reduced quantitatively in multi‐drug‐resistant (MDR) variants cells, according to extent drug resistance. In addition, determinant de...
The present study reports on the use of gene transfer by retrovirus-derived shuttle vectors in generation hybrid hybridomas secreting bispecific monoclonal antibodies. neo- and dhfr- genes were infected into distinct murine hybridomas, thus conferring a dominant resistance trait to geneticin (G418) methotrexate. employed replication-deficient dependent complementation helper virus provided irradiated packaging lines. After cocultivation with relevant cell lines, stable hybridoma lines...
Primary or essential hyperhidrosis is a disorder characterized by excessive sweating beyond physiological needs. It common disease (with an incidence of up to 2.8%) that causes intense discomfort for patients. Video-assisted thoracoscopic bilateral sympathectomy effective surgical treatment with high success rates and improvement in quality life. In the last decade, advantages single-port approach have become clear. Problems intraoperative bleeding management been solved using thoracoscopes...
Abstract Objective To assess the minimally invasive single-port thoracoscopic sympathicotomy feasibility and efficacy in patients with treatment-resistant RP. Methods Single-port was performed unilaterally on left side eight RP (six males, two females, a median age of 45.2 years). Five had primary three secondary Perfusion effects hands were assessed at baseline after 1 month by using cooling recovery procedure, laser speckle contrast analysis. Number duration attacks reported over 2-week...
OBJECTIVESTraining models are essential in mastering the skills required for off-pump coronary artery bypass grafting (OPCAB). We describe a new, high-fidelity, effective and reproducible beating-heart OPCAB training model human cadavers.
Mediastinitis and sternum dehiscence are serious complications after open heart surgery, causing an increase in hospital stay, utilization of health care resources, mortality. The defect that results sternal wound debridement frequently necessitates tissue-flap coverage, for which pectoralis major transposition currently is the preferred method. In this study, postoperative outcome individual characteristics patients undergoing were analyzed to identify predictors closure.A retrospective...