A5081038901- Galectins and Cancer Biology
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Signaling Pathways in Disease
- PI3K/AKT/mTOR signaling in cancer
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- HER2/EGFR in Cancer Research
- Genomics and Chromatin Dynamics
- Toxin Mechanisms and Immunotoxins
- Molecular Biology Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Ophthalmology and Eye Disorders
- Neurological Disease Mechanisms and Treatments
- Neurological Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lipoproteins and Cardiovascular Health
- Genetic factors in colorectal cancer
- Thyroid Disorders and Treatments
- Mechanisms of cancer metastasis
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
Abu Dhabi Health Services
2024
University of Bergen
2016-2024
Ain Shams University
2009-2023
King Salman International University
2022
Haukeland University Hospital
2021
Heidelberg University
2009-2015
DKFZ-ZMBH Alliance
2010-2015
German Cancer Research Center
2010
Epithelial to mesenchymal transition (EMT) is a well-characterized process of cell plasticity that may involve metabolic rewiring. In cancer, EMT associated with malignant progression, tumor heterogeneity, and therapy resistance. this study, we investigated the role succinate dehydrogenase (SDH) as potential key regulator EMT.Associations between SDH subunits were explored in gene expression data from breast cancer patient cohorts, followed by in-depth studies suppression mediator cultured...
beta-Catenin is a pivotal molecule of the Wnt-signalling pathway, involved in regulation developmental and oncogenic processes as well intercellular adhesion. So far, beta-catenin has been thought to be regulated mainly at protein level. Here, we provide evidence for transcriptional mechanism invasion front colorectal liver metastases. In nude mouse/LS174T cell xenograft model metastases, observed up-regulation mRNA levels 13.7-fold increase promoter activity cancer cells front. addition,...
MicroRNAs (miRNAs) have been repeatedly shown to play important roles in liver pathologies, including hepatitis, cirrhosis, and cancer. Egypt has the highest hepatitis C virus (HCV) infection rate worldwide, predominantly involving genotype-4.In this study, we attempted characterize miRNA profile of poorly studied genotype 4 HCV chronically infected Egyptian patients obtain a better understanding disease its complications help design management protocols.We analyzed expression levels...
Acute myeloid leukemia (AML) is the most common type of in adults with lowest survival rate all leukemias. It a heterogeneous disease which variety cytogenetic and molecular alterations have been identified. Some galectins were previously reported to important roles cancer-like neoplastic transformation, tumor cell survival, angiogenesis, metastasis. Previous studies showed that some galectin family members play role various types leukemia. The present study aims at evaluating clarifying...
Missense mutations in PIK3CA are common breast cancers. They mostly involve exons 9 and 20 which encode kinase helical domains of the protein may result its activation. activating were previously shown to predict lower pathologic complete response (pCR) HER2-positive cancer cases undergoing neoadjuvant human epidermal growth factor receptor 2-targeting therapy. Hence, present work was conducted estimate mutation frequency 51 patients by direct sequencing. Our results showed 8 out (15.7%)...
The present study was conducted to investigate the effect of γ-radiation alone or combined with a cytotoxic drug, simvastatin, on viability and cell cycling myeloma line. P3NS1 cells were treated selected dose simvastatin (0.1 μM/l) 24 hours prior γ-irradiation (0.25, 0.5 1 Gy). viability, induction apoptosis, death, cycling, generation ROS, expression P53, Bax, Bcl2, caspase3, PARP1 Fas genes estimated. results indicated that treatment for γ- irradiation increased death 37.5% as compared...
Abstract Two functional SNPs (SNP285G > C; rs117039649 and SNP309T G; rs2279744) have previously been reported to modulate Sp1 transcription factor binding the promoter of proto-oncogene MDM2 , influence cancer risk. Recently, a third SNP (SNP55C T; rs2870820) was also affect transcription. In this large population based case-control study, we genotyped SNP55 in 10,779 Caucasian individuals, for SNP309 SNP285, including cases colon (n = 1,524), lung 1,323), breast 1,709) prostate 2,488)...
Abstract Background: Despite recent advances in personalized medicine, conventional chemotherapy remains a backbone breast cancer therapy. Thus, identifying markers predicting sensitivity or resistance to individual chemotherapeutics is of great importance. Methods: In the EpiTax neoadjuvant trial, enrolling patients between 1997-2003, with primary cancers (T2 >4cm, T3/T4 and/or N2/N3) were randomized epirubicin 90mg/m2/3W paclitaxel 200mg/m²/3W monotherapy, cross-over case inferior...
Galectins have been recently tackled by many researchers in the field of cancer due to their role tumorigenesis, disease progression, and metastasis. Thus, they are currently involved biomarkers research on several types cancer. In ovarian cancers, few studies were carried out evaluate galectins expression profiling. Hence, our present study was executed mRNA -1, -3, -4, -8, -9 epithelial cancers.Fifty-six tumor samples carcinomas analyzed for using qRT-PCR, fold-changes calculated...
While large GWAS analyses have not found convincing associations between MDM2 promoter SNP55 and gynaecological cancers, is in linkage disequilibrium with two other functional SNPs the same promoter, likely to obscure single cancer risk. Here, we assessed impact of on risk endometrial ovarian cancer, including sub-analyses stratified for region.Using a custom LightSNiP assay, genotyped hospital-based cohorts patients (n = 1,332) 1,363) compared genotypes healthy female controls 1,858).Among...
Galectin-4 is a member of the galectin family which consists 15 galactoside-binding proteins. Previously, galectin-4 has been shown to have role in cancer progression and metastasis it found upregulated many solid tumours, including colorectal (CRC). Recently, metastatic process was suggested be via promoting cells adhere blood vascular endothelium. In present study, regulatory region LGALS4 (galectin-4) seven colon cell lines investigated with respect genetic variation that could linked...
A worldwide shortage of molecular biology consumables is in surge. This includes filter tips, nucleic acid purification kits, polymerases, reverse-transcriptase, and different types reagents which are included viral diagnostic kits. In developing countries, the problem even worse, since there few capital enterprise to adopt this kind industry. So, our aim develop a suitable, functional, comparable commercial ones, affordable in-house protocol purify RNA. We sought some published RNA...
Grave's ophthalmopathy is a prevalent complication of disease. In this case report, we present an 84-year-old female patient with no symptoms hyperthyroidism except that ophthalmopathy. The patient's thyroid profile was normal, and both receptor antibodies thyroid-stimulating immunoglobulins were negative. MRI showed thickening the medial inferior recti muscles in eyes. With absence masses on MRI, diagnosis highly suspicious. Regular monitoring done for year, remained euthyroid negative...
Abstract As a consequence of Covid-19 pandemic, the basic lab consumables are in shortage, especially low-income countries. Thus, main objective present study is to develop and evaluate homemade solution isolate plasmid. To pursue this objective, RNase A was overexpressed Bl21 DE3 cells ( E. coli strain) prepared as crude refolding reaction with proper activity. Also, lysis buffers, neutralization buffer, washing buffers were prepared. The miniprep kit showed successful isolation px48SpCas9...
Galectins are sticky molecules that bind to β-galactoside. Their interactions render them essential players in many cellular processes. The imbalance of galectin expression was reported diseases. In cancer, galectins interact with the extracellular matrix, evade immune system, and potentially have broad blood components. last ten years, since 2010, we did focus on research different cancer types. Our findings showed an interaction between cells erythrocytes via galectin-4. Moreover, found...
Abstract Covid-19 crisis did hit many socio-economic aspects in the whole world. In scientific research, problem is getting even worse, since most of materials and consumable are allocated to health sector. Many research laboratories around world have big delay receiving their purchases accomplish projects. developing countries, situation much more difficult, funding resources directed there a notable increase reagents’ prices. Therefore, aim present study make homemade reagents for RNA...
Twenty years ago, working with molecular biology research was very costly worldwide. Later on, after distributing the know-how of many reagents, it becomes more feasible to work in this field reasonable costs. In addition, optimization large-scale production enzymes, kits, lab consumables, and reagents made a big revolution diagnostics. So, having affordable technologies any laboratory is an aim itself, especially developing countries. After Covid-19 crisis, need surge. However, consumables’...