Huanglongbing (HLB, or citrus greening) is the most destructive disease of citrus, which associated with Candidatus Liberibacter asiaticus (Las). Few management options are available, aside from preventive measures such as removing infected plants, planting disease-free seedlings, and managing insect vector. In this study, we assessed efficacy thermotherapy against HLB under controlled greenhouse conditions. A total 60 two-year-old, graft-infected Citrus reticulata Blanco plants were used....

Citrus melanose, caused by Diaporthe citri, is one of the most economically significant fungal diseases in China. Given that application fungicides main technique used for control citrus many studies have focused on enhancing effectiveness and efficiency use this disease. Here, we developed a TaqMan quantitative polymerase chain reaction (qPCR) protocol monitoring melanose via detection suspected infected leaves airborne conidia D. citri. A primer pair probe targeting citri were designed to...

Arbuscular mycorrhizal (AM) fungi can improve plant phosphorus (P) uptake; however, information about how AM affect rhizosphere organic acid and microbial activity to alleviate citrus low P stress is limited. Here, a pot experiment was conducted evaluate the effect of (Rhizophagus intraradices, Ri) inoculation on content, biomass (MB) enzyme trifoliate orange (Poncirus trifoliata L. Raf.) seedlings grown under three conditions. The results showed that all recorded higher concentrations,...

Chinese bayberry (Myrica rubra Siebold & Zucc.), an evergreen fruit tree, is widely grown in southern China. In 1999, severe twig dieback was observed on M. Taizhou and it spread to several major Rubra-producing areas of Zhejiang covering more than 6,000 ha by 2011. Symptoms were usually from June November first appeared as chlorosis leaves leaf drop, followed the formation dark brown lesions covered with white mycelia surrounding scars. The can extend whole tree causing discoloration xylem....

A brown leaf spot disease was observed on Chinese bayberry (Myrica rubra) in Zhejiang Province, China during 2012 and 2014. fungus isolated from diseased leaves showing typical symptoms of spot. The pathogenicity the confirmed through inoculation experiments. Based morphological characteristics molecular analyses, pathogen identified as Pestalotiopsis clavispora (G. F. Atk.) Steyaert. This is first report P. causing China.

In this study, two polyclonal antibodies were produced against the Omp protein of 'Candidatus Liberibacter asiaticus'. First, omp genes sequenced to exhibit 99.9% identity among 137 isolates collected from different geographical origins. Then, peptides containing hydrophobic polypeptide-transport-associated (POTRA) domain and β-barrel domain, respectively, identified on protein. After that, these overexpressed in Escherichia coli purified by affinity chromatography immunize white rabbits....

(2020). Virulence of Purpureocillium lilacinum strain ZJPL08 and efficacy a wettable powder formulation against the Asian citrus psyllid (Diaphorina citri) Biotechnology & Biotechnological Equipment: Vol. 34, No. 1, pp. 1104-1113.

Citrus diseases caused by fungal pathogens drastically decreased the yield and quality of citrus fruits, leading to huge economic losses. Given threats chemical pesticides on environment human health, biocontrol agents have received considerable attention worldwide as ecofriendly sustainable alternative fungicides. In present study, we isolated a

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The most serious citrus postharvest diseases are green mould caused by Penicillium digitatum and blue italicum. Marine microorganisms have become a new measure in the control of these diseases. In this study, we isolated 56 marine actinomycetes, strain AM-4 from Drupa granulata exhibited strongest antagonistic activity against P. digitatum. Molecular, morphological, cultural physio-biochemical analysis identified as Streptomyces chumphonensis. Strain also 11 other phytopathogens, with an...

Citrus reticulata Blanco cv. Nian Ju, an important ornamental plant, is traditionally displayed during the Chinese Spring Festival because its golden fruits are a symbol of auspiciousness. In spring 2012, foliar blight was observed on 10 to 30% Ju plants at four nurseries in Yangjiang, Guangdong Province, China. Initial symptoms appeared as brown black lesions, followed by expansion spots into blight. Some young branches also had necrosis. During frequent rainfall and prolonged wet periods...

Huanglongbing (HLB) is a major threat to world citrus production. In this study, we investigated using heat treatment technique for managing HLB-affected trees in the field. A total of 72 5-to-8-year old mandarin citrus, Citrus reticulata Blanco, were used study. Nine regarded as replicate or block with four replicates per treatment. randomized complete design was field experimental design. The treated covered by plastic sheeting 6-h during day time, repeated three times weekly....

Huanglongbing (HLB, citrus greening) is the most destructive disease of citrus. The associated with “Candidatus Liberibacter asiaticus”. Few management options are available, besides preventive measures such as removal affected plants, planting disease-free stock and maintaining vector-free production in quarantine areas. In this study, we assessed efficacy thermotherapy against under controled laboratory conditions. A total sixty, 2-year old graft-infected Citrus reticulate Blanco...

Citrus Huanglongbing (HLB) is a devastating disease within the industry. Candidatus Liberibacter asiaticus (CLas) one of most prevalent HLB-associated strains that has not been cultured in vitro. To ensure accuracy and comparability molecular diagnostic method for HLB detection, certified reference materials urgently need to be developed CLas detection. Here, we series DNA using 16S rDNA as target gene SAND gene. The fragment cloned by NCBI sequence extracted healthy leaves are thoroughly...

Mancozeb is extensively used fungicide to prevent citrus melanose in most Asian countries, especially China. So far, however, there have been no reports of thet effect on the rhizosphere bacterial community. Therefore, this comparative experiment defined genomic and functional related community soil health 2-years old Citrus unshiu Marc. through amplicon sequencing chemical analysis. This study evaluated mancozeb properties citrus-cultivated richness diversity We also investigated abundance...

Abstract Satsuma mandarin is one of the most widely cultivated citrus in world. In this study, we firstly presented complete chloroplast genome by using BGISEQ-500 sequencing technology. The 160,699 bp size, including a pair inverted repeat regions 26,990 bp, large single copy region 87,918 and small 18,801 bp. contains 108 unique genes, 75 protein coding 29 tRNA genes 4 rRNA genes. Phylogenetic analysis based on showed that relationship between reticulate was closest.