A5068522892- Neurobiology and Insect Physiology Research
- Plant and animal studies
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Animal Behavior and Reproduction
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
Neurobehavioral Systems
2019-2024
University of California, Los Angeles
2018-2023
Center for Neuro-Oncology
2019-2020
To visualize the cellular and subcellular localization of neuromodulatory G-protein–coupled receptors in Drosophila , we implement a molecular strategy recently used to add epitope tags ionotropic at their endogenous loci. Leveraging evolutionary conservation identify sites more likely permit insertion tag, generated constitutive conditional tagged alleles for 5-HT1A 5-HT2A 5-HT2B Oct β 1R 2R two isoforms OAMB mGluR . The allow restricted expression receptor specific cell types, an option...
The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. mechanisms by which inhibition SERT activity influences behavior are poorly understood. To address this question in model organism Drosophila melanogaster , we developed new loss function mutations ( dSERT) . Previous studies both flies mammals have implicated as an important neuromodulator sleep, our newly generated dSERT mutants show increase total...
Sensory systems rely on neuromodulators, such as serotonin, to provide flexibility for information processing stimuli vary, light intensity throughout the day. Serotonergic neurons broadly innervate optic ganglia of Drosophila melanogaster, a widely used model studying vision. It remains unclear whether serotonin modulates physiology interneurons in ganglia. To address this question, we first mapped expression patterns receptors visual system, focusing subset cells with processes ganglion,...
The transcriptional effects of SSRIs and other serotonergic drugs remain unclear, in part due to the heterogeneity postsynaptic cells, which may respond differently changes signaling. Relatively simple model systems such as Drosophila afford more tractable microcircuits investigate these specific cell types. Here, we focus on mushroom body, an insect brain structure heavily innervated by serotonin comprised multiple different but related subtypes Kenyon cells. We use fluorescence-activated...
To visualize the cellular and subcellular localization of neuromodulatory G-protein coupled receptors (GPCRs) in
Abstract Sensory systems rely on neuromodulators, such as serotonin, to provide flexibility for information processing in the face of a highly variable stimulus space. Serotonergic neurons broadly innervate optic ganglia Drosophila melanogaster , widely used model studying vision. The role serotonergic signaling lobe and mechanisms by which serotonin regulates visual remain unclear. Here we map expression patterns receptors system, focusing subset cells with processes first ganglion, lamina,...
The transcriptional effects of SSRIs and other serotonergic drugs remain unclear, in part due to the heterogeneity postsynaptic cells, which may respond differently changes signaling. Relatively simple model systems such as Drosophila afford more tractable microcircuits investigate these specific cell types. Here, we focus on mushroom body, an insect brain structure heavily innervated by serotonin comprised multiple different but related subtypes Kenyon cells. We use fluorescence activated...
Monoamine neurotransmitters such as noradrenalin are released from both synaptic vesicles (SVs) and large dense-core (LDCVs), the latter mediating extrasynaptic signaling. The contribution of versus signaling to circuit function behavior remains poorly understood. To address this question, we have previously used transgenes encoding a mutation in Drosophila Vesicular Transporter (dVMAT) that shifts amine release SVs LDCVs. circumvent use with non-endogenous patterns expression, now...
Abstract The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. mechanisms by which inhibition SERT activity influences behavior are poorly understood. To address this question in model organism Drosophila melanogaster , we developed new loss function mutations ( dSERT). Previous studies both flies mammals have implicated as an important neuromodulator sleep, our newly generated dSERT mutants show increase...