Abstract Starch plays a central role in the life cycle as one of principal sources chemical energy. This polysaccharide accumulates plastids green algae and land plants, both organisms have acquired various enzyme isoforms for each step metabolic pathway. Eukaryotic microalgae present critical photosynthetic functions higher plants. However, due to small size their genome, gene redundancy is decreased, feature that makes them an excellent model investigating properties physiology. In last...

ABSTRACT Serratia marcescens is able to invade, persist, and multiply inside nonphagocytic cells, residing in nonacidic, nondegradative, autophagosome-like vacuoles. In this work, we have examined the physiological role of PhoP/PhoQ system its function control critical virulence phenotypes S. . We demonstrated involvement adaptation bacterium growth on scarce environmental Mg 2+ , at acidic pH, presence polymyxin B. also shown that these conditions constitute signals activate system. found...

Lignocellulose is the most abundant natural biopolymer on earth and a potential raw material for production of fuels chemicals. However, only some organisms such as bacteria fungi produce enzymes that metabolize this polymer. In work we have demonstrated presence cellulolytic activity in supernatant Scenedesmus quadricauda cultures identified extracellular cellulases genome five species. green alga which grows both freshwater saltwater regions well soils, showing highly flexible metabolic...

ABSTRACT Rob is a member of the Sox/Mar subfamily AraC/XylS-type transcriptional regulators implicated in bacterial multidrug, heavy metal, superoxide, and organic solvent resistance phenotypes. We demonstrate that, Salmonella enterica , overexpression upregulates transcription mgtA which codes for MgtA Mg 2+ transporter. was previously characterized as -modulated PhoPQ regulon. Here we that (but not its paralog protein SoxS or MarA) able to induce PhoP-independent fashion by binding...

Starch-binding domains are key modules present in several enzymes involved polysaccharide metabolism. These non-catalytic have already been described as essential for starch-binding and the catalytic activity of starch synthase III from higher plant Arabidopsis thaliana. In Ostreococcus tauri, a unicellular green alga Prasinophyceae family, there three SSIII isoforms, known Ostta SSIII-A, SSIII-B SSIII-C. this work, using silico vitro characterization techniques, we demonstrated that SSIII-C...

Salmonella enterica polymyxin B (PM) resistance is modulated mainly by substitutions of the acyl chains and phosphate groups on lipid A moiety lipopolysaccharide. These modifications are mediated genes under control PmrA/PmrB PhoP/PhoQ two-component regulatory systems. In this study, a deletion in gene encoding alternative sigma(54) factor, rpoN, was shown to increase PM without affecting protamine sensitivity. The results presented here showed that increased observed DeltarpoN mutant occurs...

Starch is the major energy storage carbohydrate in photosynthetic eukaryotes. Several enzymes are involved building highly organized semi-crystalline starch granules, including starch-synthase III (SSIII), which widely conserved organisms. This enzyme catalyzes extension of α-1,4 glucan chain and plays a regulatory role synthesis starch. Interestingly, unlike most plants, unicellular green alga Ostreococcus tauri has three SSIII isoforms. In present study, we describe structure function...

Abstract Scenedesmus quadricauda is an important, rapidly growing, freshwater microalga explored as a source of alternative fuel because its significantly high lipid content. However, the molecular basis fatty acid biosynthesis scarcely elucidated not only in S. but also whole. This study aims to understand 3D model structure enzymes involved synthesis, and their catalytic sites compared other species. The first genome sequence was carried initially our isolate LWG002611 (GenBank ID...

Lignocellulose is the most abundant natural biopolymer on earth and a potential raw material for production of fuels chemicals. However, only some organisms such as bacteria fungi produce necessary enzymes to metabolize it. In this work we detected presence extracellular cellulases in genome five species Scenedesmus . These microalgae grow both, freshwater saltwater regions well soils, displaying highly flexible metabolic properties. The comparison sequences different with hydrolytic from...

The physicochemical properties of starches from different botanical origin, such as viscosity, gelatinization temperature, solubility and degradability depend on the amylose/amylopectin ratio length frequency α-1,6-glycosidic bonds in amylopectin. These branches action starch branching enzymes (SBEs), which are highly structurally conserved plants. We recently identified a novel gene Ostreococcus tauri (OsttaSBEIII) codes for protein showing activity with structure than other known SBEs...

Scenedesmus is an important, rapidly growing, freshwater microalga because of its significantly high lipid content. However, the molecular basis fatty acid biosynthesis scarcely elucidated not only in but also microalgae as a whole. Mining genome Scenedesmus, we identified some crucial enzymes involved pathways such acetyl-CoA carboxylase (ACC), malonyl-CoA:ACP transacylase (MAT) and fatty-acyl thioesterases (FAT). Detailed comparative analyses these demonstrated highly significant sequence...

Scenedesmus is an important, rapidly growing, freshwater microalga because of its significantly high lipid content. However, the molecular basis fatty acid biosynthesis scarcely elucidated not only in but also microalgae as a whole. Mining genome Scenedesmus, we identified some crucial enzymes involved pathways such acetyl-CoA carboxylase (ACC), malonyl-CoA:ACP transacylase (MAT) and fatty-acyl thioesterases (FAT). Detailed comparative analyses these demonstrated highly significant sequence...

Background: The debranching starch enzymes, isoamylase 1 and 2 are well-conserved enzymes present in almost all the photosynthetic organisms. These involved crystallization process of key components which remove misplaced α-1,6 ramifications on final molecule. Aim: In this work, we performed a functional structural study novel from Ostreococcus tauri . Methods: We identified conserved amino acid residues possibly catalysis. also region at N-terminal end that resembles Carbohydrate Binding...

Lignocellulose is the most abundant natural biopolymer on earth and a potential raw material for production of fuels chemicals. However, only some organisms such as bacteria fungi produce necessary enzymes to metabolize it. In this work we detected presence extracellular cellulases in genome five species Scenedesmus . These microalgae grow both, freshwater saltwater regions well soils, displaying highly flexible metabolic properties. The comparison sequences different with hydrolytic from...