A5017385164- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Photoreceptor and optogenetics research
- Neuropeptides and Animal Physiology
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Retinal Diseases and Treatments
- Nicotinic Acetylcholine Receptors Study
University of California System
2024
University of California, Irvine
2022-2024
Abstract Rhodopsin is a prototypical G protein-coupled receptor (GPCR) critical for vertebrate vision. Research on GPCR signaling states has been facilitated using llama-derived nanobodies (Nbs), some of which bind to the intracellular surface allosterically modulate receptor. Extracellularly binding allosteric have also investigated, but structural basis their activity not resolved date. Here, we report library Nbs that extracellular rhodopsin and thermodynamics its activation process....
Chronic, progressive retinal diseases, such as age-related macular degeneration (AMD), diabetic retinopathy, and retinitis pigmentosa, arise from genetic environmental perturbations of cellular tissue homeostasis. These disruptions accumulate with repeated exposures to stress over time, leading visual impairment and, in many cases, legal blindness. Despite decades research, therapeutic options for the millions patients suffering these disorders remain severely limited, especially treating...
The ultrafast photochemical reaction mechanism, transient spectra, and transition kinetics of the human blue cone visual pigment have been recorded at room temperature. Ultrafast time-resolved absorption spectroscopy revealed progressive formation decay several metastable photo-intermediates, corresponding to Batho Meta-II photo-intermediates previously observed with bovine rhodopsin green opsin, on picosecond millisecond timescales following pulsed excitation. experimental data reveal...
Binding of an extracellular signaling molecule to a G protein-coupled receptor (GPCR) results in protein activation, which turn triggers the production second messengers. Although many advances have been achieved understanding GPCR early steps this process are still poorly understood. In case rhodopsin, paradigmatic member class A GPCRs, endogenous ligand is retinal covalently bound K296 through Schiff base. The activation rhodopsin triggered by absorption photon and isomerization...