A5014085653- Barrier Structure and Function Studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Caveolin-1 and cellular processes
- Neonatal and fetal brain pathology
- MicroRNA in disease regulation
- Axon Guidance and Neuronal Signaling
- Traumatic Brain Injury and Neurovascular Disturbances
- Angiogenesis and VEGF in Cancer
- Drug Transport and Resistance Mechanisms
- S100 Proteins and Annexins
- Lipid metabolism and disorders
- Anesthesia and Neurotoxicity Research
- Glioma Diagnosis and Treatment
- Diet and metabolism studies
- Extracellular vesicles in disease
- Gastrointestinal motility and disorders
- Immune cells in cancer
- Congenital heart defects research
- Neuroscience of respiration and sleep
- Hedgehog Signaling Pathway Studies
- Gut microbiota and health
University of Bari Aldo Moro
2014-2024
The Sense Innovation and Research Center
2021
GenXPro (Germany)
2019
University of Kentucky
2019
University of Copenhagen
2019
Université Laval
2017
National Cancer Institute
2017
University Hospital of Zurich
2008-2011
Ospedale Pediatrico Giovanni XXIII
2009
University of Foggia
2009
Gliomas, particularly glioblastoma multiforme, perturb the blood-brain barrier and cause brain edema that contributes to morbidity mortality. The mechanisms underlying this vasogenic are poorly understood. We examined effects of cocultured primary cultured human cells glioma-derived growth factors on endothelial cell tight junction proteins claudin 1, 5, occludin, zonula occludens 1 brain-derived microvascular a umbilical vein line. Cocultured (e.g. transforming factor β2) enhanced...
Nanotubular structures, denoted tunneling nanotubes (TNTs) have been described in recent times as involved cell-to-cell communication between distant cells. Nevertheless, TNT-like, long filopodial processes had already the last century connecting facing, growing microvessels during process of cerebral cortex vascularization and collateralization. Here we investigated possible presence cellular origin TNTs normal brain also highly vascularized tumors.We searched for by high-resolution...
Capillaries of peritumoral and normal brain tissues were ultrastructurally morphometrically investigated to evaluate the changes in capillaries connected with tumor-associated vasogenic edema. The endothelial cells showed varying thickness, electron-lucent cytoplasm, structurally tight junctions. basal lamina was thickened, rarefied, vacuolated. pericytes provided pinocytotic vesicles phagocytic bodies. astrocytic glia appeared empty or swollen, few glycogen granules a disarranged...
P-Glycoprotein (P-gp) is an ATP-dependent efflux transporter that extrudes non-polar molecules, including cytotoxic substances and drugs, from the cells. It was initially found in cancer cells then shown to be a normal component of complex transport systems working at blood-brain barrier (BBB). Previous studies have demonstrated that, brain, P-gp localized on luminal plasmalemma BBB endothelial it may interact with caveolar compartment these The aim this study identify site cellular...
The pathophysiology of cerebral cortical lesions in multiple sclerosis (MS) is not understood. We investigated cortex mi-crovessels during immune-mediated demyelination the MS model chronic murine experimental autoimmune encephalomyelitis (EAE) by immunolocalization endothelial cell tight junction (TJ) integral proteins claudin-5 and occludin, a structural protein caveolae, caveolin-1, blood-brain barrier-specific transporter, Glut 1. In EAE-affected mice, there were areas extensive subpial...
Intestinal fibrosis is a complication of inflammatory bowel disease [IBD]. Although fibrostenosis rare event in ulcerative colitis [UC], there evidence that fibrotic rearrangement the colon occurs later stages. This retrospective study aimed at examining histopathological features colonic wall both short-lasting [SL] and long-lasting [LL] UC.Surgical samples left from non-stenotic SL [≤ 3 years, n = 9] LL [≥ 10 10] UC patients with active were compared control tissues cancer without [n 12]...
During experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis associated with blood-brain barrier (BBB) disruption, oligodendrocyte precursor cells (OPCs) overexpress proteoglycan nerve/glial antigen 2 (NG2), proliferate, and make contacts the microvessel wall. To explore whether OPCs may actually be recruited within neurovascular unit (NVU), de facto intervening in its cellular molecular composition, we quantified by immunoconfocal morphometry presence of contact...
In myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE), several areas of demyelination are detectable in mouse cerebral cortex, where neuroinflammation events associated with scarce inflammatory infiltrates and blood-brain barrier (BBB) impairment. this condition, the administration mesenchymal stem cells (MSCs) controls neuroinflammation, attenuating astrogliosis promoting acquisition cell traits by astrocytes. To contribute to understanding...
F3/contactin (CNTN1) and TAG-1 (CNTN2) are closely related axonal glycoproteins that differentially regulated during development. In the cerebellar cortex is expressed first as granule cell progenitors differentiate in premigratory zone of external germinal layer. However, these cells begin radial migration, replaced by F3/contactin. To address significance this differential regulation, we have generated transgenic mice which expression driven gene regulatory sequences, results premature...
During brain development and blood-brain barrier (BBB) differentiation the expression of P-glycoprotein (P-gp) may complement protective function placental against xenobiotic substances. To establish an immunohistochemical procedure for P-gp detection, different anti-P-gp monoclonal antibodies were first tested on a fibrosarcoma cell line colonic carcinoma tissue. The protocol was then adult human brains as BBB-P-gp tissue-specific control double labeling with astroglia marker glial...
Experimentally induced autoimmune encephalomyelitis (EAE) in mice provides an animal model that shares many features with human demyelinating diseases such as multiple sclerosis (MS). To what extent the cerebral cortex is affected by process of demyelination and how corollary response oligodendrocyte lineage explicated are still not completely known aspects EAE. By performing a detailed situ analysis expression myelin markers we have identified areas subpial animals conventionally EAE...
Patients with Parkinson's disease (PD) are often characterized by functional gastrointestinal disorders. Such disturbances can occur at all stages of PD and precede the typical motor symptoms many years. However, morphological alterations associated intestinal in undetermined. This study examined remodelling colonic wall 6-hydroxydopamine (6-OHDA)-induced rats. 8 weeks after 6-OHDA injection animals were sacrificed. Inflammatory infiltrates, collagen deposition epithelial barrier tunica...
Mounting evidence has linked the metabolic disease to neurovascular disorders and cognitive decline. Using a murine model of high-fat high-sugar diet mimicking obesity-induced type 2 diabetes mellitus (T2DM) in humans, we show that pro-inflammatory mediators altered immune responses damage blood-brain barrier (BBB) structure, triggering proinflammatory phenotype. We find disruption tight junctions basal lamina due loss control production matrix metalloproteinases (MMPs) their inhibitors...
HEAL9 alleviates cognitive decline and normalizes colonic motility in the prodromal AD via microbiota-gut-inflammasome-brain axis. can represent a suitable therapeutical option for treatment of early related intestinal symptoms.
ABSTRACT Introduction : The molecular mechanism of immune‐mediated necrotizing myopathy (IMNM) remains unknown. Autophagy impairment, described in autoimmune diseases, is a key process myofiber protein degradation flux and muscle integrity has not been studied IMNM. Methods Muscle biopsies from patients with IMNM ( n = 40), dermatomyositis (DM; 24), polymyositis (PM; 8), mitochondrial pathology (4), sporadic inclusion body myositis (8), controls (6) were compared by immunohistochemistry....
P-Glycoprotein (P-gp) is a 170-kDa transmembrane glycoprotein that works as an efflux pump and confers multidrug resistance (MDR) in normal tissues tumors, including nervous brain tumors. In the developing telencephalon, endothelial expression of P-gp, subcellular localization transporter at luminal cell (EC) plasma membrane are early hallmarks blood-brain barrier (BBB) differentiation suggest functional BBB activity may complement placental function P-gp blood-placental interface. fetal...