A5084146374- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Immune Response and Inflammation
- Hormonal Regulation and Hypertension
- Adipose Tissue and Metabolism
- Anesthesia and Neurotoxicity Research
- Tryptophan and brain disorders
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- Heart Rate Variability and Autonomic Control
- interferon and immune responses
- Epigenetics and DNA Methylation
- Hepatocellular Carcinoma Treatment and Prognosis
- Cardiac Ischemia and Reperfusion
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Cancer, Stress, Anesthesia, and Immune Response
- Immune cells in cancer
- Cardiac Health and Mental Health
- Fibroblast Growth Factor Research
- Neuropeptides and Animal Physiology
- Intensive Care Unit Cognitive Disorders
- Circadian rhythm and melatonin
University of Cincinnati
2014-2019
University of Cincinnati Medical Center
2016-2019
Lahey Hospital and Medical Center
2018
Tufts University
2018
Oregon Health & Science University
2009-2015
Oncovir (United States)
2015
University of Portland
2013
Hewlett-Packard (United States)
2013
Morehouse School of Medicine
2012
Lipopolysaccharide (LPS) preconditioning provides neuroprotection against subsequent cerebral ischemic injury through activation of its receptor, Toll-like receptor 4 (TLR4). Paradoxically, TLR by endogenous ligands after ischemia worsens stroke damage. Here, we define a novel, protective role for TLRs in the context LPS preconditioning. Microarray analysis brains collected 24 h revealed unique set upregulated genes LPS-pretreated animals. Promoter gene identified an overrepresentation type...
Systemic administration of Toll-like receptor (TLR) 4 and TLR9 agonists before cerebral ischemia have been shown to reduce ischemic injury by reprogramming the response brain stroke. Our goal was explore mechanism TLR-induced neuroprotection determining whether a TLR7 agonist also protects against stroke injury.C57Bl/6, TNF(-/-), interferon (IFN) regulatory factor 7(-/-), or type I IFN (IFNAR)(-/-) mice were subcutaneously administered Gardiquimod (GDQ) 72 hours middle artery occlusion....
Organismal stress initiates a tightly orchestrated set of responses involving complex physiological and neurocognitive systems. Here, we present evidence for glucagon-like peptide 1 (GLP-1)-mediated paraventricular hypothalamic circuit coordinating the global response. The GLP-1 receptor ( Glp1r ) in mice was knocked down neurons expressing single-minded 1, transcription factor abundantly expressed nucleus (PVN) hypothalamus. Mice with 1-mediated knockdown had reduced...
Preconditioning with a low dose of harmful stimulus prior to injury induces tolerance subsequent ischemic challenge resulting in neuroprotection against stroke. Experimental models preconditioning primarily focus on neurons as the cellular target cerebral protection, while less attention has been paid cerebrovascular compartment, whose role pathogenesis brain is crucial. We have shown that polyinosinic polycytidylic acid (poly-ICLC) protects damage. To delineate mechanism poly-ICLC we...
Background The medial prefrontal cortex is necessary for appropriate appraisal of stressful information, as well coordinating visceral and behavioral processes. However, prolonged stress impairs function prefrontal-dependent behaviors. Additionally, chronic induces sympathetic predominance, contributing to health detriments associated with autonomic imbalance. Previous studies identified a subregion rodent cortex, infralimbic (IL), key regulator neuroendocrine-autonomic integration after...
Preconditioning induces ischemic tolerance, which confers robust protection against damage. We show marked with polyinosinic polycytidylic acid (poly-IC) preconditioning in three models of murine ischemia-reperfusion injury. Poly-IC induced ischemia modeled vitro brain cortical cells and vivo renal ischemia. Further, unlike other Toll-like receptor (TLR) ligands, generally induce significant inflammatory responses, poly-IC elicits only modest systemic inflammation. Results that is a new...
In response to an acute threat homeostasis or well-being, the hypothalamic-pituitary-adrenocortical (HPA) axis is engaged. A major outcome of this HPA activation mobilization stored energy, fuel appropriate behavioral and/or physiological perceived threat. Importantly, extent activity thought be modulated by individual’s nutritional environment. study, we report that manipulations signaling a relative depletion dietary carbohydrates, thereby inducing ketosis, acutely and chronically activate...
Systemic preconditioning with the TLR9 ligand CpG induces neuroprotection against brain ischemic injury through a tumor necrosis factor (TNF)-dependent mechanism. It is unclear how systemic administration of engages to induce protective phenotype. To address this, we created TLR9-deficient reciprocal bone marrow chimeric mice lacking on either hematopoietic cells or radiation-resistant nonhematopoietic origin. We report that wild-type reconstituted failed show after preconditioning. Further,...
Preconditioning with poly-l-lysine and carboxymethylcellulose (ICLC) provides robust neuroprotection from cerebral ischemia in a mouse stroke model. However, the receptor that mediates is unknown. As synthetic double-stranded RNA, poly-ICLC may bind endosomal Toll-like 3 or one of cytosolic retinoic acid-inducible gene-I-like family members, gene-I, melanoma differentiation-associated protein 5. Activation these receptors culminates type I interferons (IFN-α/β) induction-a response required...
Ischemic tolerance is a biological process that can be utilized to unlock the brain's own endogenous protection mechanisms and, as such, holds true promise for patients at risk of ischemic injury. Experimentally, preconditioning with various Toll-like receptor (TLR) agonists has now been demonstrated successfully attenuate damage, partly through genomic reprogramming body's response stroke. This treatment diminishes inflammatory stroke and same time enhances production anti-inflammatory...
508 Background: For HCC patients undergoing liver transplantation (LT), local treatment as a "bridge" is standard to decrease tumor burden. The most common TACE, but the best and cheapest bridging modality unclear. We prospectively compared SBRT TACE bridge for LT. then cost of two strategies. Methods: From 9/2014-1/2017, 31 within Milan Criteria with C-P Class A/B cirrhosis were randomized vs. SBRT. received 2 treatments one month apart utilizing DEBDOX beads (n = 17) hospitalized symptom...
Organismal stress initiates a tightly orchestrated set of responses involving complex physiological and neurocognitive systems.Here, we present evidence for glucagon-like peptide 1 (GLP-1)-mediated paraventricular hypothalamic circuit coordinating the global response.The GLP-1 receptor (Glp1r) in mice was knocked down neurons expressing single-minded 1, transcription factor abundantly expressed nucleus (PVN) hypothalamus.Mice with 1-mediated Glp1r knockdown had reduced...
ABSTRACT Perhaps the most salient behaviors that individuals engage in involve avoidance of aversive experiences and pursuit pleasurable experiences. Engagement these is regulated to a significant extent by an individual's hormonal milieu. For example, glucocorticoid hormones are produced hypothalamic‐pituitary‐adrenocortical (HPA) axis, influence aspects behavior. In turn, many can HPA axis activity. These bidirectional interactions not only coordinate physiological behavioral states each...
Introduction: Preconditioning with a TLR3 ligand, polyinosinic polycytidylic acid (poly-ICLC), synthetic dsRNA, protects against middle cerebral artery occlusion (MCAO). Using an in vitro model of the blood brain barrier (BBB) we found that Poly-ICLC stabilizes BBB during ischemia through mechanism involving IFNβ production from glial cells. We assessed hypothesis stabilization and subsequent reduction ischemic injury requires presence on both endothelial Methods: was modeled using transwell...
The prefrontal cortex is necessary for appropriate appraisal and processing of stressful information a component the central network coordinating visceral behavioral functions. In addition, prolonged stress induces sympathetic predominance, suggesting that circuitry may prevent chronic stress‐induced autonomic imbalance. We have previously identified subregion rodent cortex, infralimbic (IL), as key regulator neuroendocrine responses to stress. current study, we sought determine how IL...