A5030923125- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Digestive system and related health
- Cancer-related gene regulation
- Pancreatic and Hepatic Oncology Research
- Amino Acid Enzymes and Metabolism
- Pluripotent Stem Cells Research
- Genomics, phytochemicals, and oxidative stress
- Polyamine Metabolism and Applications
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic factors in colorectal cancer
- bioluminescence and chemiluminescence research
- Sexual Differentiation and Disorders
- IL-33, ST2, and ILC Pathways
- Cellular Mechanics and Interactions
- Esophageal Cancer Research and Treatment
University of Southern California
2021-2025
Harvard University
2015-2025
Dana-Farber Cancer Institute
2015-2025
Brigham and Women's Hospital
2016-2021
Northwestern University
2010-2011
University of Illinois Urbana-Champaign
2006-2008
Illinois College
2004-2008
University of Illinois Chicago
2007
Peoria campus of the University of Illinois System
2004
Atopic dermatitis is an allergic inflammatory skin disease characterized by the production of type 2 cytokines in innate lymphoid cells (ILC2s) and T helper (T
Lgr5(+) intestinal stem cells (ISCs) drive epithelial self-renewal, and their immediate progeny-intestinal bipotential progenitors-produce absorptive secretory lineages via lateral inhibition. To define features of early transit from the ISC compartment, we used a microfluidics approach to measure selected stem- lineage-specific transcripts in single cells. We identified two distinct cell populations, one that expresses known markers second, abundant population simultaneously mature...
Abstract A hallmark of cells comprising the superficial zone articular cartilage is their expression lubricin, encoded by Prg4 gene, that lubricates joint and protects against development arthritis. Here, we identify Creb5 as a transcription factor specifically expressed in chondrocytes required for TGF-β EGFR signaling to induce expression. Notably, forced derived from deep confers competence signals Chromatin-IP ATAC-Seq analyses have revealed directly binds two promoter-proximal...
Significance Pancreatic ductal adenocarcinoma (PDA) is a highly lethal malignancy with no effective therapies. PDA aggressiveness partly stems from its ability to grow within uniquely dense stroma restricting nutrient access. This study demonstrates that clones survive chronic deprivation acquire reversible nongenetic adaptations allowing them switch between metabolic states optimal for growth under nutrient-replete or nutrient-deprived conditions. One contributing factor this adaptation...
The expression of matrix metalloproteinases (MMPs), particularly MMP-9, is significantly increased during tumor progression and thought to play a major role in mediating angiogenic process. Since microvasculature plays an important controlling growth, we investigated the effects MMP-9 inhibition on endothelial cell migration tube formation, two determinants angiogenesis. Adenoviral-mediated downregulation inhibited wounding spheroid assays. To determine reduction glioblastoma/endothelial...
Compacted chromatin and nucleosomes are known barriers to gene expression; the nature relative importance of other transcriptional constraints remain unclear, especially at distant enhancers. Polycomb repressor complex 2 (PRC2) places histone mark H3K27me3 predominantly promoters, where its silencing activity is well documented. In adult tissues, enhancers lack H3K27me3, it unknown whether intergenic deposits affect nearby genes. primary intestinal villus cells, we identified hundreds...
Matrix Metalloproteinase-9 (MMP-9) consists of a prodomain, catalytic domain with 3 fibronectin-like type II modules and C-terminal hemopexin-like (PEX) domain. These domains play distinct roles in terms proteolytic activity, substrate binding interaction inhibitors receptors. To assess the potential MMP-9-PEX to interfere tumor progression, we stably transfected human glioblastoma cells an expression vector containing cDNA sequence MMP-9-PEX. The selected clones exhibited decreased MMP-9...
Steroidogenic factor 1 (SF-1) is essential for the development and function of steroidogenic tissues. Stable incorporation SF-1 into embryonic stem cells (SF-1-ES cells) has been shown to prime steroidogenesis. When provided with exogenous cholesterol substrate, after treatment retinoic acid cAMP, SF-1-ES produce progesterone but do not other steroids such as cortisol, estradiol, or testosterone. In this study, we explored culture conditions that optimize SF-1-mediated differentiation ES...
After acquiring competence for selected cell fates, embryonic primordia may remain plastic variable periods before tissue identity is irrevocably determined (commitment). We investigated the chromatin basis these developmental milestones in mouse endoderm, a with recognizable rostro–caudal patterning and transcription factor (TF)-dependent interim plasticity. Foregut-specific enhancers are as accessible active early midgut foregut intestinal established only after ectopic cis -regulatory...
Transcription factors guide tissue development by binding to developmental stage-specific targets and establishing an appropriate enhancer landscape. In turn, DNA chromatin modifications direct the genomic of transcription factors. However, how navigate features selectively bind a small subset all possible target loci remains poorly understood. Here we show that Cdx2—a lineage defining factor binds distinct in developing versus adult intestinal epithelial cells—has preferential affinity for...
The intestinal epithelium is a rapidly regenerating tissue dependent on resident stem cell self-renewal and differentiation. reactive oxygen species (ROS) generating enzyme NADPH oxidase 1 (NOX1) spatially restricted to the crypt base in distal colon. Using NOX1-deletion, along with cell-specific redox reporter mice, lineage tracing, single-cell transcriptomics, cysteine proteomics, metabolic studies, we investigated role of balance colonic function. We show that cells uniquely require...
Abstract Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in United States, despite established treatment options and knowledge causal mutations. CRC involves significant rewiring cellular chromatin alongside mutational accumulation, which fuel aberrant transcriptional programs. Many developmental gene regulatory mechanisms are also activated during early tumorigenesis. However, details molecular controls tumor development evolution not fully understood. Mutations...
Epidemiological studies have indicated that increased consumption of cruciferous vegetables is associated with a statistically significant reduction in the risk for cancers. The major bioactive agent these class sulfur-containing glycosides called glucosinolates. Isothiocyanates, derivatives glucosinolates, been shown to possess anticancer properties variety tumor cell lines. In this study, we evaluated antigrowth, cycle modulation and proapoptotic effects isothiocyanate iberin human...
Neuroblastomas are the most common extra-cranial tumors of childhood and well known for their heterogeneous clinical behavior associated with certain genetic aberrations. Radiation therapy is an important modality treatment high-risk neuroblastomas. In this study, we investigated whether ionizing irradiation modulate migration invasiveness human neuroblastoma cells expression proangiogenic molecules to be involved in tumor progression metastasis. Irradiation resulted increased invasion as...
Intestinal metaplasia (Barrett's esophagus, BE) is the principal risk factor for esophageal adenocarcinoma (EAC). Study of basis BE has centered on intestinal factors, but loss identity likely also reflects absence key squamous-cell factors. As few determinants stratified epithelial cell-specific gene expression are characterized, it important to identify necessary transcription factors.We tested regional mRNAs all putative DNA-binding proteins in mouse digestive tract and verified...