A5042326983- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Plant-Microbe Interactions and Immunity
- DNA Repair Mechanisms
- Toxin Mechanisms and Immunotoxins
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Cancer Mechanisms and Therapy
- Plant Disease Resistance and Genetics
Furman University
2019-2022
University of North Carolina at Chapel Hill
2012
Abstract Background Cryptococcus neoformans , a basidiomycetous yeast, is fungal pathogen that can colonize the lungs of humans causing pneumonia and meningitis in severely immunocompromised individuals. Recent studies have implied antifungal drug fluconazole (FLC) induce oxidative stress C. by increasing production reactive oxygen species (ROS), as presence antioxidant ascorbic acid (AA) could reverse inhibitory effects FLC on . However, Candida albicans AA has been shown to stimulate...
ABSTRACT Cryptococcus neoformans is a leading cause of fungal meningitis in immunocompromized populations. Amphotericin B (AMB) and fluconazole (FLC) are common anticryptococcal agents. AMB treatment leads to severe side-effects. In contrast, FLC-based therapy relatively safe, although C. often develops resistance this drug. must adapt the challenging environment human host. Environmental effects on potency FLC development drug remain poorly characterized. Here, nutrients, temperature...
Abstract The human fungal pathogen Cryptococcus neoformans relies on a complex signaling network for the adaptation and survival at host temperature. Protein phosphatase calcineurin is central to proliferation 37°C but its exact contributions remain ill-defined. To better define genetic C. temperature tolerance, 4031 gene knockouts were screened genes essential under conditions that keep inactive. Identified 83 candidate strains, potentially sensitive 37°C, subsequently subject...
Cryptococcus neoformans is an encapsulated yeast, found in the lungs of immunocompromised hosts. In human host, Cryptococcusis subject to multiple forms environmental stress, including heat stress. Heat shock protein 90 (Hsp90) essential that has been shown protect fungal cells against extreme temperature host and assist proper cell division. Hsp90 also implicated resistance anti-fungal drug fluconazole. To better characterize role fluconazole Cryptococcuswe addressed two questions: 1. Does...
Understanding development of resistance to anti-fungal drugs in the pathogenic fungus, Cryptococcus neoformans, is crucial for improving anticryptococcal therapy. We have previously determined that presence anti-oxidants growth medium can reverse fungal inhibition caused by azole drug, fluconazole. However, host/pathogen response co-treatment with fluconazole and remains poorly characterized. In our study, we used assays, infection assays using a heterologous host model, Minimum Inhibitory...