A5101995274- Evolution and Paleontology Studies
- Nanopore and Nanochannel Transport Studies
- Microbial Natural Products and Biosynthesis
- Alkaloids: synthesis and pharmacology
- Evolution and Genetic Dynamics
- Microfluidic and Bio-sensing Technologies
- Microbial Metabolism and Applications
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Genomics and Phylogenetic Studies
- Groundwater flow and contamination studies
- Ion-surface interactions and analysis
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Toxoplasma gondii Research Studies
- Heat shock proteins research
- Trypanosoma species research and implications
- Bat Biology and Ecology Studies
- Venomous Animal Envenomation and Studies
- Ecology and Vegetation Dynamics Studies
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Genetic diversity and population structure
- Microfluidic and Capillary Electrophoresis Applications
- Malaria Research and Control
Natural History Museum
2003-2023
Imperial College London
2015-2022
Molecular Sciences Institute
2019-2020
Lane College
2020
Nanopipettes were used for real-time investigation into actin dynamics and drug binding at single-molecule resolution, showing promise a better understanding of the mechanism protein–protein interactions discovery.
Supertree methods (SMs) are techniques for inferring (super)trees from sets of (input) trees. Classical consensus SMs that were designed the special case where input trees have identical leaf sets. The need can also combine information with nonidentical has led to many alternative SMs. Some these generalizations conservative (strict and semistrict) do not resolve tree conflicts (e.g., Gordon, 1986; Goloboff Pol, 2002). Our focus here is on more liberal SMs, those capable resolving among...
Although technical definitions exist for various support metrics, the notion of per se has received little explicit attention. Thus, despite its widespread use in phylogenetics, “support” is absent from glossaries and/or indices several recent texts (e.g., Kitching et al., 1998; Page and Holmes, Schuh, 2001). Farris al. (2001) recently argued that interpreting branch lengths trees as indicative corresponding groups both common unfortunate. To their claim, they presented two contrived...
Advances in synthetic biology have enabled the production of a variety compounds using bacteria as vehicle for complex compound biosynthesis. Violacein, naturally occurring indole pigment with antibiotic properties, can be biosynthetically engineered Escherichia coli expressing its nonnative synthesis pathway. To explore whether this biosynthesis platform could used drug discovery, here we screened bacterially derived violacein against main causative agent human malaria, Plasmodium falciparum .
Proteins of the actin-depolymerizing factor (ADF)/cofilin family have been shown to be crucial for motility and survival apicomplexan parasites. However, mechanisms by which ADF proteins fulfill their function remain poorly understood. In this study, we investigate comparative activities from Toxoplasma gondii Plasmodium falciparum, human malaria parasite, using a conditional T. ADF-knockout line complemented with variants either species. We show that P. falciparum ADF1 can fully restore...
Polymerization of the cytosolic protein actin is critical to cell movement and host invasion by malaria parasite, Plasmodium falciparum. Any disruption polymerization dynamics will render parasite incapable invading a thereby unable cause infection. Here, we explore potential using truncated latrunculins as chemotherapeutics for treatment malaria. Exploration binding interactions natural inhibitor with revealed how core could retain its key interaction features actin. This was synthesized...
Correction for ‘Single-molecule nanopore sensing of actin dynamics and drug binding’ by Xiaoyi Wang <italic>et al.</italic>, <italic>Chem. Sci.</italic>, 2020, <bold>11</bold>, 970–979, DOI: 10.1039/C9SC05710B.
ABSTRACT Advances in synthetic biology have enabled production of a variety compounds using bacteria as vehicle for complex compound biosynthesis. Violacein, naturally occurring indole pigment with antibiotic properties, can be biosynthetically engineered Escherichia coli expressing its non-native synthesis pathway. To explore whether this biosynthesis platform could used drug discovery, here we screened bacterially-derived violacein against the main causative agent human malaria, Plasmodium...
Abstract Actin is a key protein in the dynamic processes within eukaryotic cell. To date, methods exploring molecular state of actin are limited to insights gained from structural approaches, providing snapshot folding, or that require chemical modifications compromising monomer thermostability. Nanopore sensing permits label-free investigation native proteins and ideally suited study such as specialised buffers cofactors. Using nanopores we determined at macromolecular level (filamentous...
The multi-subunit chaperonin containing TCP-1 (CCT) is an essential molecular chaperone that functions in the folding of key cellular proteins. This paper reviews interactome eukaryotic CCT and its primary clients, ubiquitous cytoskeletal proteins, actin tubulin. interacts with other nascent especially WD40 propeller also assists assembly several protein complexes. A new proteomic dataset presented for purified from human malarial parasite, P. falciparum (PfCCT). CCT8 subunit gene was...