A5071085945- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Renal and related cancers
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Ruminant Nutrition and Digestive Physiology
- Bioinformatics and Genomic Networks
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Biofuel production and bioconversion
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Animal Genetics and Reproduction
- RNA modifications and cancer
University of Pennsylvania
2018-2024
Immunoblotting experiments with polyclonal and monoclonal anti-transcription factor IIIA (TFIIIA) antibodies reveal different electrophoretic forms of TFIIIA in extracts from immature mature oocytes Xenopus laevis. The well-characterized 39-kD species is present approximately 10(12) copies per cell stage I-III previtellogenic declines abundance by 10- to 20-fold during oogenesis. An immunologically related protein apparent molecular mass 42 kD at 2-4% the level oocytes, this increases...
Pluripotency can be induced in somatic cells by the expression of four "Yamanaka" factors OCT4, KLF4, SOX2, and MYC. However, even homogeneous conditions, usually only a rare subset admit reprogramming, molecular characteristics this remain unknown. Here, we apply retrospective clone tracing to identify characterize individual human fibroblast that are primed for reprogramming. These fibroblasts showed markers increased cell cycle speed decreased activation. Knockdown activation factor...
Abstract Non-genetic factors can cause individual cells to fluctuate substantially in gene expression levels over time. Yet it remains unclear whether these fluctuations persist for much longer than the time of one cell division. Current methods measuring single mostly rely on point measurements, making duration or cellular memory difficult measure. Here, we report a method combining Luria and Delbrück’s fluctuation analysis with population-based RNA sequencing (MemorySeq) identifying genes...
Abstract Molecular differences between individual cells can lead to dramatic in cell fate, such as death versus survival of cancer upon drug treatment. These originating remain largely hidden due difficulties determining precisely what variable molecular features which cellular fates. Thus, we developed Rewind, a methodology that combines genetic barcoding with RNA FISH directly capture rare give rise behaviors interest. Applied BRAF V600E melanoma, trace drug-resistant fates back...
Abstract Organoids recapitulate complex 3D organ structures and represent a unique opportunity to probe the principles of self-organization. While we can alter an organoid’s morphology by manipulating culture conditions, organoid often resembles that its original organ, suggesting morphologies are governed set tissue-specific constraints. Here, establish framework identify constraints on morphological features quantifying them from microscopy images organoids exposed range perturbations. We...