A5007143388- Pain Mechanisms and Treatments
- Intensive Care Unit Cognitive Disorders
- Anesthesia and Pain Management
- Botulinum Toxin and Related Neurological Disorders
- Ship Hydrodynamics and Maneuverability
- Acupuncture Treatment Research Studies
- Pain Management and Opioid Use
- Cavitation Phenomena in Pumps
- Anesthesia and Sedative Agents
- Neuropeptides and Animal Physiology
- Pediatric Pain Management Techniques
- Anesthesia and Neurotoxicity Research
- Hydraulic and Pneumatic Systems
- Cardiac, Anesthesia and Surgical Outcomes
- Maritime Navigation and Safety
- Nerve injury and regeneration
- Electric Motor Design and Analysis
- Hereditary Neurological Disorders
- Fluid Dynamics Simulations and Interactions
- Structural Integrity and Reliability Analysis
- Gut microbiota and health
- Trigeminal Neuralgia and Treatments
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Magnetic Properties and Applications
Renji Hospital
2018-2025
Shanghai Jiao Tong University
2013-2025
Binzhou University
2024-2025
Binzhou Medical University
2024-2025
Soochow University
2020-2024
Rutgers, The State University of New Jersey
2015-2020
State Key Laboratory of Oncogene and Related Genes
2020
Soochow University
2020
Second Affiliated Hospital of Soochow University
2020
Rutgers New Jersey Medical School
2018
Abstract Nerve injury induces changes in gene transcription dorsal root ganglion (DRG) neurons, which may contribute to nerve injury-induced neuropathic pain. DNA methylation represses expression. Here, we report that peripheral increases expression of the methyltransferase DNMT3a injured DRG neurons via activation factor octamer 1. Blocking this increase prevents voltage-dependent potassium (Kv) channel subunit Kcna2 promoter region and rescues attenuates Conversely, absence injury,...
Accumulating evidence suggests that activation of spinal microglia contributes to the development inflammatory and neuropathic pain. However, role in maintenance chronic pain remains controversial. Bone cancer shares features pain, but temporal astrocytes this model is not well defined. Here, we report an unconventional advanced-phase bone a female rat model. elicited delayed persistent microglial dorsal horn on days 14 21, day 7. In contrast, induced rapid astrocytic 7–21. Spinal inhibition...
Opioids are the gold standard for pharmacological treatment of neuropathic pain, but their analgesic effects unsatisfactory in part due to nerve injury-induced downregulation opioid receptors dorsal root ganglia (DRG) neurons. How injury drives such remains elusive. DNA methyltransferase (DNMT)-triggered methylation represses gene expression. We show here that blocking increase DRG DNMT3a (a de novo DNMT) rescued expression Oprm1 and Oprk1 mRNAs respective encoding mu-opioid receptor (MOR)...
Abstract Nerve injury-induced downregulation of voltage-gated potassium channel subunit Kcna2 in the dorsal root ganglion (DRG) is critical for DRG neuronal excitability and neuropathic pain genesis. However, how nerve injury causes this still elusive. Euchromatic histone-lysine N-methyltransferase 2, also known as G9a, methylates histone H3 on lysine residue 9 to predominantly produce a dynamic dimethylation, resulting condensed chromatin gene transcriptional repression. We showed here that...
Expressional changes of pain-associated genes in primary sensory neurons DRG are critical for neuropathic pain genesis. DNA methyltransferase (DNMT)-triggered methylation silences gene expression. We show here that DNMT1, a canonical maintenance methyltransferase, acts as the <i>de novo</i> DNMT and is required genesis likely through repressing at least <i>Kcna2</i> expression male mice. Peripheral nerve injury upregulated DNMT1 injured transcription factor cAMP response element binding...
Pain is the most common symptom of bone cancer. TGF-β, a major bone-derived growth factor, largely released by osteoclast resorption during progression cancer and contributes to proliferation, angiogenesis, immunosuppression, invasion, metastasis. Here, we further show that TGF-β1 critical for cancer-induced pain sensitization. We found that, after cancer, was highly expressed in tumor-bearing bone, expression its receptors, TGFβRI TGFβRII, significantly increased DRG rat model based on...
Peripheral nerve injury-induced changes in gene transcription and translation primary sensory neurons of the dorsal root ganglion (DRG) are considered to contribute neuropathic pain genesis. Transcription factors control expression. injury increases expression myeloid zinc finger protein 1 (MZF1), a factor, promotes its binding voltage-gated potassium 1.2 (Kv1.2) antisense (AS) RNA injured DRG. However, whether DRG MZF1 participates is still unknown. Here, we report that blocking increase...
Neuropathic pain, a distressing and debilitating disorder, is still poorly managed in clinic. Opioids, like morphine, remain the mainstay of prescribed medications treatment this but their analgesic effects are highly unsatisfactory part due to nerve injury-induced reduction opioid receptors first-order sensory neurons dorsal root ganglia. G9a repressor gene expression. We found that increases its catalyzed repressive marker H3K9m2 responsible for epigenetic silencing Oprm1, Oprk1, Oprd1...
The transmission of normal sensory and/or acute noxious information requires intact expression pain-associated genes within the pain pathways nervous system. Expressional changes these after peripheral nerve injury are also critical for neuropathic induction and maintenance. Methyl-CpG-binding domain protein 1 (MBD1), an epigenetic repressor, regulates gene transcriptional activity. We report here that MBD1 in primary neurons DRG is genesis as MBD1-deficient mice exhibit reduced responses to...
Antineoplastic drugs induce dramatic transcriptional changes in dorsal root ganglion (DRG) neurons, which may contribute to chemotherapy‐induced neuropathic pain. K 2p 1.1 controls neuronal excitability by setting the resting membrane potential. Here, we report that systemic injection of chemotherapy agent paclitaxel time‐dependently downregulates expression mRNA and its coding protein DRG neurons. Rescuing this downregulation mitigates development maintenance paclitaxel‐induced mechanical...
Postoperative pain is common in pediatric urological surgery. The study assess the impact of perioperative intravenous infusion low-dose esketamine on postoperative
Biological membrane channels, considered as molecular gatekeepers, control the transportation of molecules and ions across live cell membranes. Developing synthetic passable channels with predictable structures, high transport efficiency, low cytotoxicity on cells is great interest for replicating functions endogenous protein but remains challenging. The development DNA nanotechnology provides possible solutions making precise structures controllable functionalization. Therefore, in this...
Biased µ-opioid receptor (MOR) agonists enhance pain relief by selectively activating G protein-coupled signaling and minimizing β-arrestin-2 activation, resulting in fewer side effects. This multicenter Phase II/III trial evaluated the optimal dosage, efficacy, safety of SHR8554, a biased MOR agonist, for postoperative management following orthopedic surgery. In II, 121 patients were divided into four groups to receive varying patient-controlled analgesia (PCA) doses SHR8554 or morphine....
Adult hippocampal neurogenesis is linked to memory formation In the adult brain, with new neurons in hippocampus exhibiting greater plasticity during their immature stages compared mature neurons. Abnormal closely associated cognitive impairment central nervous system diseases. Targeting and regulating have been shown improve deficits. This review aims expand current understanding prospects of targeting treatment impairment. Recent research indicates presence abnormalities AHN several...
Systemically administered dexmedetomidine (DEX), a selective α2 adrenergic receptor (α2-AR) agonists, produces analgesia and sedation. Peripherally restricted α2-AR antagonist could block the analgesic effect of systemic DEX on neuropathic pain, with no sedation, indicating peripheral DEX. Tetrodotoxin-resistant (TTX-R) sodium channel Nav1.8 play important roles in conduction nociceptive sensation. Both are found small DRG neurons. We, therefore, investigated effects currents acutely...
Sickle cell disease is associated with acute painful episodes and chronic intractable pain. Endothelin-1, a known pain inducer, elevated in the blood plasma of both sickle patients mouse models disease. We show here that levels endothelin-1 its endothelin type A receptor are increased dorsal root ganglia model Pharmacologic inhibition or neuron-specific knockdown receptors primary sensory neurons alleviated basal post-hypoxia evoked hypersensitivities mice. Mechanistically, contribute to...
Abstract Background Postoperative cognitive dysfunction ( POCD ) is consistently associated with increased morbidity and mortality, which has become a major concern of patients caregivers. Although occurs mainly in aged patients, it happens at any age. Previous studies demonstrated that anesthesia/surgery had no effects on reference memory adult mice. However, whether impairs working remains unclear. Working deficit would result many deficits executive function. We hypothesized impaired the...
Background: Although major joint replacement surgery has a high overall success rate, postoperative cognitive dysfunction (POCD) is common complication after anesthesia and surgery, increasing morbidity mortality. Identifying POCD risk factors would be helpful to prevent decrease the occurrence of POCD. We hypothesized that preoperative chronic pain increases Methods: A single-center, observational, prospective cohort study was conducted from January 2018 March 2020. All consecutive elderly...