A5042662232- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Bacteriophages and microbial interactions
- Bone health and osteoporosis research
- Chemical Synthesis and Analysis
- Bone Metabolism and Diseases
- Coronary Interventions and Diagnostics
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Blood groups and transfusion
- Mental Health and Psychiatry
- Cardiac, Anesthesia and Surgical Outcomes
- Immunodeficiency and Autoimmune Disorders
- Bone health and treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood disorders and treatments
- Alkaline Phosphatase Research Studies
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Health and Disease Prevention
- Genomics and Rare Diseases
Universität Innsbruck
2021-2025
Innsbruck Medical University
2012-2025
University of Bern
2012-2018
Institut de Biologie Moléculaire et Cellulaire
2013
Nonprotein coding RNA (ncRNA) molecules have been recognized recently as major contributors to regulatory networks in controlling gene expression a highly efficient manner. These RNAs either originate from their individual transcription units or are processing products longer precursor RNAs. For example, tRNA-derived fragments (tRFs) identified all domains of life and represent growing, yet functionally poorly understood, class ncRNA candidates. Here we present evidence that tRFs the...
Posttranscriptional processing of RNA molecules is a common strategy to enlarge the structural and functional repertoire RNomes observed in all 3 domains life. Fragmentation basically classes has been reported yield smaller non-protein coding RNAs (ncRNAs) that typically possess different roles compared with their parental transcripts. Here we show valine tRNA-derived fragment (Val-tRF) produced under certain stress conditions halophilic archaeon Haloferax volcanii capable binding small...
The alizarin red S assay is considered the gold standard for quantification of osteoblast mineralization and thus widely used among scientists. However, there are several restrictions to this method, e.g., moderate sensitivity makes it difficult uncover slight but significant effects potentially clinically relevant substances. Therefore, an adaptation staining method appropriate might be obtained by increasing ability osteoblasts. In study, cell culture experiments with human (SaOs-2) murine...
Cigarette smoking is one of the most important and preventable risk factors for atherosclerosis. However, because complex composition cigarette smoke, detailed pathophysiological mechanisms are not fully understood. Based on controversial reports pro-atherogenic activity smoke condensate, also called tar fraction (CSC), we decided to analyse effects CSC viability endothelial cells in vitro. The results this study show that low concentrations hydrophobic induces DNA damage resulting a...
Accumulating recent evidence identified the ribosome as binding target for numerous small and long non-protein-coding RNAs (ncRNAs) in various organisms of all 3 domains life. Therefore it appears that ribosome-associated ncRNAs (rancRNAs) are a prevalent, yet poorly understood class cellular transcripts. Since rancRNAs associated with arguable most central enzyme cell seems plausible to propose role translation control. Indeed first experimental on has been presented, linking association...
Regulation of gene expression at the translational level allows rapid adaptation cellular proteomes to quickly changing environmental conditions and is thus central for prokaryotic organisms. Small non-coding RNAs (sRNAs) have been reported effectively orchestrate translation control in bacteria archaea mainly by targeting mRNAs partial base complementarity. Here we report an unprecedented mechanism how sRNAs are capable modulating protein biosynthesis halophilic archaeon Haloferax volcanii....
Self-cleaving ribozymes are biologically relevant RNA molecules which catalyze site-specific cleavage of the phosphodiester backbone. Gathering knowledge their three-dimensional structures is critical toward an in-depth understanding function and chemical mechanism. Equally important collecting information on folding process inherent dynamics a ribozyme fold. Over past years, Selective-2'-Hydroxyl Acylation analyzed by Primer Extension (SHAPE) turned out to be significant tool probe...
Inherited platelet disorders (IPDs) are a heterogeneous group of rare entities caused by molecular divergence in genes relevant for formation and function. A rational diagnostic approach is necessary to counsel treat patients with IPDs. With the introduction high-throughput sequencing at beginning this millennium, more accurate diagnosis IPDs has become available. We discuss advantages limitations genetic testing, technical issues, ethical aspects. Additionally, we provide information on...
Introduction Hemophilia A (HA) patients are prone to low bone mineral density (BMD), independent of commonly known risk factors [1]. Recent research suggests that coagulation factor VIII (FVIII) could potentially act through direct interactions with key bone-regulating pathways (e.g. RANK/RANKL/OPG axis, Wnt/ß-catenin pathway, cytokines) or indirectly downstream processes thrombin production) [2]. Despite these promising leads, the connection between FVIII and health remains elusive....
Background/Objectives: Hemophilia A is associated with frequent bleeding episodes, joint damage, and reduced bone mineral density (BMD). The role of coagulation factors inflammatory cytokines on metabolism, particularly osteoblast function, increasing interest. However, significant inter-species differences in remodeling raise concerns about the translatability findings from murine models to human systems. This study aims investigate effects formation, focusing cell viability mineralization...
Introduction Patients with severe haemophilia A do not only suffer from spontaneous bleeding into joints or soft tissues, but they are also prone to low bone mineral density (BMD) independent of commonly accepted risk factors including smoking and reduced physical activity1,2. Nowadays evidence accumulates that FVIII plays a role outside the coagulation system3. may directly interact main players physiology, receptor activator nuclear factor kappa-B (RANK)/RANK ligand (RANKL)/osteoprotegerin...