A5049515947- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Advanced Electron Microscopy Techniques and Applications
- Animal Virus Infections Studies
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Lipid Membrane Structure and Behavior
- Particle accelerators and beam dynamics
Texas A&M University
2017-2024
The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen Pseudomonas aeruginosa . single-stranded RNA (ssRNA) phage PP7 binds to T4P and brought cell surface through retraction. Using fluorescence microscopy, we discovered that detaches T4P, which impairs motility restricts pathogen’s virulence. cryo–electron mutagenesis, optical trapping, Langevin dynamics simulation, resolved structure PP7, PP7/T4P complex showed detachment driven by affinity...
Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and proteins (rProtein). Here, we present the near-atomic structures of Mtb 50S subunit complete 70S ribosome, solved by cryo-electron microscopy. Upon joining large small subunits, a 100-nt long segment 23S rRNA, named H54a or 'handle', switches interactions with rRNA helix H68 rProtein uL2 to bS6, forming new intersubunit bridge 'B9'. In 70S, B9 is mostly maintained, leading...
The coat proteins (CPs) of single-stranded RNA bacteriophages (ssRNA phages) directly assemble around the genomic (gRNA) to form a near-icosahedral capsid with single maturation protein (Mat) that binds gRNA and interacts retractile pilus during infection host. Understanding assembly ssRNA phages is essential for their use in biotechnology, such as protection delivery. Here, we present complete model phage Qβ, revealing 3' untranslated region Mat 4127 nucleotides fold domain-by-domain,...
Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into interaction between Acinetobacter phage AP205 pili. Using cryo-electron microscopy, we solve structures virion an asymmetric dimer maturation proteins, native bearing distinct...
Abstract Membrane‐enclosed transport carriers sort biological molecules between stations in the cell a dynamic process that is fundamental to physiology of eukaryotic organisms. While much known about formation and release from specific intracellular membranes, mechanism carrier recycling endosome, compartment central cellular signaling, remains be resolved. In Caenorhabditis elegans , endosome requires dynamin‐like, Eps15‐homology domain (EHD) protein, RME‐1, functioning with...