A5041055707- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Influenza Virus Research Studies
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- SARS-CoV-2 and COVID-19 Research
- Acoustic Wave Resonator Technologies
- Electrowetting and Microfluidic Technologies
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- Advanced Fiber Optic Sensors
- COVID-19 Clinical Research Studies
- Neuroscience and Neural Engineering
- Mechanical and Optical Resonators
- Molecular Junctions and Nanostructures
- Molecular Biology Techniques and Applications
- 3D Printing in Biomedical Research
- Immune cells in cancer
KU Leuven
2013-2024
Flu is caused by the influenza virus that, due to mutations, keeps our body vulnerable for infections, making early diagnosis essential. Although immuno-based diagnostic tests are available, they have low sensitivity and reproducibility. In this paper, prospect of detecting A using digital ELISA has been studied. To appropriately select bioreceptors bioassay, seven commercial antibodies against nucleoprotein were methodically tested their reactivity binding affinity. The study performed on...
The ongoing COVID-19 pandemic has emphasized the urgent need for rapid, accurate, and large-scale diagnostic tools. Next to this, significance of serological tests (i.e., detection SARS-CoV-2 antibodies) also became apparent studying patients' immune status past viral infection. In this work, we present a novel approach not only measuring antibody levels but profiling binding kinetics complete polyclonal response against receptor domain (RBD) spike protein, an aspect possible achieve with...
Medical diagnostics is moving toward disease-related target detection at very low concentrations because of the (1) quest for early-stage diagnosis, a point where only limited amounts are present, (2) trend minimally invasive sample extraction, yielding samples containing target, and (3) need straightforward collection, usually resulting in volume collected. Hence, diagnostic tools allowing ultrasensitive point-of-care (POC) crucial simplified timely diagnosis many illnesses. Therefore, we...
Current aptamer selection procedures enable limited control and transparency on how the DNA pool is evolving. Affinity tests binding analyses are not always informative. Here we show that real-time PCR provides a valuable tool for follow-up of selection. Limited time, work amount amplified ssDNA make this an interesting instrument to set-up SELEX design monitor enrichment oligonucleotides. reMelting Curve Analysis (rMCA) after reannealing under stringent conditions information about...
In the continuous combat against diseases, there is need for tools that enable an improved diagnostic efficiency towards higher information density combined with reduced time-to-result and cost. Here, a novel fully integrated microfluidic platform, Evalution™, evaluated as potential solution to this need. Encoded microparticles channel-based microfluidics allow fast, sensitive simultaneous detection of several disease-related biomarkers. Since binary code represented by physically present...
To date, surface plasmon resonance (SPR) biosensors have been exploited in numerous different contexts while continuously pushing boundaries terms of improved sensitivity, specificity, portability and reusability. The latter has attracted attention as a viable alternative to disposable biosensors, also offering prospects for rapid screening biomolecules or biomolecular interactions. In this context here, we developed an approach successfully regenerate fiber-optic (FO)-SPR when utilizing...
Aptamers are short, single stranded DNA/RNA oligonucleotides with the ability to fold into well-defined three-dimensional structures, which fundamental for their highly selective target recognition, rivaling thus antibodies in terms of affinity and specificity. Thanks these properties, they have been integrated different micro- nanoscale transducers, resulting a new type biosensors, known as `aptasensors', used truly generation diagnostic tools. Here, an overview will be given aptamer...