A5039643202- Ion channel regulation and function
- Heart Rate Variability and Autonomic Control
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Neuroscience of respiration and sleep
- Metabolomics and Mass Spectrometry Studies
- Protein Kinase Regulation and GTPase Signaling
- Mitochondrial Function and Pathology
- Cardiac electrophysiology and arrhythmias
- Health and Lifestyle Studies
- Diet, Metabolism, and Disease
- Neuroendocrine regulation and behavior
- Biochemical Analysis and Sensing Techniques
- Lipid Membrane Structure and Behavior
- PI3K/AKT/mTOR signaling in cancer
- Mental Health Research Topics
- Bioinformatics and Genomic Networks
- Diet and metabolism studies
- Cannabis and Cannabinoid Research
- Retinal Development and Disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
University of Washington
2019-2024
Howard Hughes Medical Institute
2023
Howard University
2023
Universidad Nacional Autónoma de México
2014-2021
Universidad Autónoma de la Ciudad de México
2018
Instituto Politécnico Nacional
2016
Metabolic disorders, such as obesity, elevated blood pressure, dyslipidemias, insulin resistance, hyperglycemia, and hyperuricemia have all been identified risk factors for an epidemic of important widespread chronic-degenerative diseases, type 2 diabetes cardiovascular disease, that constitute some the world's most public health challenges. Their increasing prevalence can be associated with ageing population to lifestyles within obesogenic environment. Age is not a controllable variable;...
Phosphoinositide membrane lipids are ubiquitous low-abundance signaling molecules. They direct many physiological processes that involve ion channels, identification, fusion of vesicles, and vesicular endocytosis. Pools these continually broken down refilled in living cells, the rates some reactions strongly accelerated by stimuli. Recent biophysical experiments described here measure model kinetics regulation lipid signals intact cells. Rapid on-line monitoring phosphoinositide metabolism...
The dynamic metabolism of membrane phosphoinositide lipids involves several cellular compartments including the ER, Golgi, and plasma membrane. There are cycles phosphorylation dephosphorylation synthesis, transfer, breakdown. simplified cycle comprises synthesis phosphatidylinositol in transport, Golgi membranes to generate 4,5-bisphosphate, followed by receptor-stimulated hydrolysis return components ER for reassembly. Using probes specific lipid species, we have analyzed kinetics these...
Metabolic homeostasis emerges from the interplay between several feedback systems that regulate physiological variables related to energy expenditure and availability, maintaining them within a certain range. Although it is well known how each individual system functions, there little research focused on integration adjustment of multiple results in generation metabolic health. The aim here was generate an integrative model metabolism, seen as network, study changes across human lifespan. We...
Phosphatidylinositol-4,5-bisphosphate (PIP2) is a membrane phosphoinositide that regulates the activity of many ion channels. Influx calcium primarily through voltage-gated (CaV) channels promotes insulin secretion in pancreatic β-cells. However, whether CaV are regulated by PIP2, as case for some non-insulin-secreting cells, unknown. The purpose this study was to investigate PIP2 depletion β-cells activation muscarinic pathway induced oxotremorine methiodide (Oxo-M). channel currents were...
Phosphoinositide lipids regulate many cellular processes and are synthesized by lipid kinases. Type I phosphatidylinositol phosphate 5-kinases (PIP5KIs) generate 4,5-bisphosphate [PtdIns(4,5)P2]. Several phosphoinositide-sensitive readouts revealed the nonequivalence of overexpressing PIP5KIβ, PIP5KIγ or Ras association domain family 4 (RASSF4), believed to activate PIP5KIs. Mass spectrometry showed that each these three proteins increased total bisphosphates (PtdInsP2) trisphosphates...
The FDA's approval of peptide drugs such as Ziconotide or Exendin for pain relief and diabetes treatment, respectively, enhanced the interest to explore novel conotoxins from
PtdIns(4,5)P2 is a signaling lipid central to the regulation of multiple cellular functions. It remains unknown how fulfills various functions in different cell types, such as regulating neuronal excitability, synaptic release, and astrocytic function. Here, we compared dynamics synthesis hippocampal neurons astrocytes with kidney-derived tsA201 line. The experimental approach was (1) measure abundance rate precursors using specific biosensors, (2) levels its mass spectrometry, (3) use...
The endocannabinoid system is found in tissues that regulate the glycemia, including adipose tissue, muscle, and pancreatic islets. Diet-induced metabolic syndrome changes expression of CB receptors liver. However, it poorly understood whether (MetS) affects β cells. We analyzed cells under chronic high-sucrose diet (HSD)-induced MetS. Wistar rats fed an HSD as a model MetS were used to investigate cannabinoid receptors. After 8 weeks treatment, we evaluated appearance following biomarkers:...
ABSTRACT Phosphatidylinositol(4,5)-bisphosphate (PtdInsP2) is an important modulator of many cellular processes, and its abundance in the plasma membrane closely regulated. We examined hypothesis that members Dishevelled scaffolding protein family can bind lipid kinases phosphatidylinositol 4-kinase (PI4K) 4-phosphate 5-kinase (PIP5K), facilitating synthesis PtdInsP2 directly from phosphatidylinositol. used several assays for to examine cooperative function phosphoinositide Dvl3 context two...
Overactivity of the sympathetic nervous system is a hallmark aging. The cellular mechanisms behind this overactivity remain poorly understood, with most attention paid to likely central components. In work, we hypothesized that aging also affects function motor neurons in peripheral ganglia. To test hypothesis, compared electrophysiological responses and ion-channel activity isolated from superior cervical ganglia young (12 weeks), middle-aged (64 old (115 weeks) mice. These approaches...
Overactivity of the sympathetic nervous system is a hallmark aging. The cellular mechanisms behind this overactivity remain poorly understood, with most attention paid to likely central components. In work, we hypothesized that aging also affects function motor neurons in peripheral ganglia. To test hypothesis, compared electrophysiological responses and ion-channel activity isolated from superior cervical ganglia young (12 weeks), middle-aged (64 old (115 weeks) mice. These approaches...
Overactivity of the sympathetic nervous system is a hallmark aging. The cellular mechanisms behind this overactivity remain poorly understood, with most attention paid to likely central components. In work, we hypothesized that aging also affects function motor neurons in peripheral ganglia. To test hypothesis, compared electrophysiological responses and ion-channel activity isolated from superior cervical ganglia young (12 weeks), middle-aged (64 old (115 weeks) mice. These approaches...
Overactivity of the sympathetic nervous system is a hallmark aging. The cellular mechanisms behind this overactivity remain poorly understood, with most attention paid to likely central components. In work, we hypothesized that aging also affects function motor neurons in peripheral ganglia. To test hypothesis, compared electrophysiological responses and ion-channel activity isolated from superior cervical ganglia young (12 weeks), middle-aged (64 old (115 weeks) mice. These approaches...