A5019643385- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Calcium signaling and nucleotide metabolism
- Photoreceptor and optogenetics research
- Cardiomyopathy and Myosin Studies
Fundación Ciencias Exactas y Naturales
2016-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2020-2024
University of Buenos Aires
2016-2024
Abstract Dynamins are large GTPases whose primary function is not only to catalyze membrane scission during endocytosis but also modulate other cellular processes, such as actin polymerization and vesicle trafficking. Recently, we reported that centronuclear myopathy associated dynamin‐2 mutations, p.A618T, p.S619L, impair Ca 2+ ‐induced exocytosis of the glucose transporter GLUT4 containing vesicles in immortalized human myoblasts. As occur within rapid timescales, here applied...
Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance secretory response at such frequencies requires a continuous replenishment releasable vesicles. However, mechanism that allows vesicle remains unclear. Here, using membrane capacitance measurements on mouse cells, we studied group vesicles released by single potential-like stimulus (APls). exocytosis triggered APls (ETAP) represents fraction (40%) immediately pool, highly...
It is widely accepted that the exocytosis of synaptic and secretory vesicles triggered by Ca2+ entry through voltage-dependent channels. However, there evidence an alternative mode induced membrane depolarization but lacking current intracellular increase. In this work we investigated if such a mechanism contributes to vesicle in mouse chromaffin cells.Exocytosis was evaluated patch-clamp capacitance measurements, carbon fibre amperometry TIRF. Cytosolic estimated using epifluorescence...
Gain-of-function mutations of dynamin-2, a mechano-GTPase that remodels membrane and actin filaments, cause centronuclear myopathy (CNM), congenital disease mainly affects skeletal muscle tissue. Among these mutations, the variants p.A618T p.S619L lead to gain function severe neonatal phenotype. By using total internal reflection fluorescence microscopy (TIRFM) in immortalized human myoblasts expressing pH-sensitive fluorescent protein (pHluorin) fused insulin-responsive aminopeptidase IRAP...
The importance of the immediately releasable pool (IRP) vesicles was proposed to reside in maintenance chromaffin cell secretion during firing action potentials at basal physiological frequencies. To accomplish this duty, IRP should be replenished as a function time. We have previously reported that an potential-like stimulus (APls) triggers release ~50% IRP, followed by fast dynamin-dependent endocytosis and associated rapid replenishment process. In work, we investigated produced after...
The maintenance of the secretory response requires a continuous replenishment releasable vesicles. It was proposed that immediately pool (IRP) is important in chromaffin cell secretion during action potentials applied at basal physiological frequencies, because proximity IRP vesicles to voltage-dependent Ca2+ channels. However, previous reports showed after depletion too slow manage such situation. In this work, we used patch-clamp measurements membrane capacitance, confocal imaging F-actin...
ABSTRACT Dynamins are large GTPases whose primary function is to catalyze membrane scission during endocytosis, but also modulate other cellular processes, such as actin polymerization and vesicle trafficking. Recently, we reported that centronuclear myopathy associated dynamin-2 mutations, p.A618T p.S619L, impair Ca 2+ -induced exocytosis of GLUT4 containing vesicles in immortalized human myoblasts. As endocytosis occur within rapid timescales, here applied high-temporal resolution...
Abstract Gain-of-function mutations of dynamin-2, a mechano-GTPase that remodels membrane and actin filaments, cause centronuclear myopathy (CNM), congenital disease mainly affects skeletal muscle tissue. Among these mutations, the variants p.A618T p.S619L lead to gain function severe neonatal phenotype. By using total internal reflection fluorescence microscopy (TIRFM) in immortalized human myoblasts expressing pH-sensitive fluorescent protein (pHluorin) fused insulin-responsive...
Abstract The importance of the immediately releasable pool (IRP) vesicles was proposed to reside in maintenance chromaffin cell secretion during firing action potentials at basal physiological frequencies. To accomplish this duty, IRP should be replenished as a function time. We have previously reported that an potential-like stimulus (APls) triggers release ∽50% IRP, followed by fast dynamin-dependent endocytosis and associated rapid replenishment process. In work we investigated produced...