A5021533160- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Retinal Diseases and Treatments
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
- Parkinson's Disease Mechanisms and Treatments
- Virus-based gene therapy research
- Neurogenesis and neuroplasticity mechanisms
- Neurological disorders and treatments
- Autism Spectrum Disorder Research
- Retinal and Macular Surgery
- Retinal Imaging and Analysis
- interferon and immune responses
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Ocular Diseases and Behçet’s Syndrome
- Manufacturing Process and Optimization
- Cytokine Signaling Pathways and Interactions
- Cellular transport and secretion
- Transcranial Magnetic Stimulation Studies
- Ocular Disorders and Treatments
Université Paris-Saclay
2020-2025
Inserm
2015-2025
Institut des Cellules Souches pour le Traitement et l'Étude des Maladies Monogéniques
2014-2023
Université d'Évry Val-d'Essonne
2007-2023
Genopole (France)
2018-2020
Association Francaise contre les Myopathies
2017-2018
Applied Functional Materials (United Kingdom)
2009
Cardiff University
2001-2006
Institut Mondor de Recherche Biomédicale
2002
Significance Human induced pluripotent stem cells (hiPSCs) could be used as an unlimited source of retinal for the treatment degenerative diseases. The production from hiPSCs personalized therapeutic approaches must comply with certain criteria, such safety, efficiency, reproducibility, and low cost. Here, we report a simple scalable differentiation process generation pigmented epithelial neural tissues containing progenitor cells. These progenitors can differentiated into all cell types,...
Human ESC–derived retinal pigment epithelial cell sheets are more effective than suspensions at rescuing photoreceptor loss in rats with dystrophy.
Abstract Dysfunction or death of retinal pigment epithelial (RPE) cells is involved in some forms Retinitis Pigmentosa and age-related macular degeneration (AMD). Since there no cure for most patients affected by these diseases, the transplantation RPE derived from human pluripotent stem (hPSCs) represents an attractive therapeutic alternative. First attempts to transplant hPSC-RPE AMD Stargardt demonstrated safety suggested potential efficacy this strategy. However, it also highlighted need...
Abstract Although viral vector‐mediated delivery of glial cell‐line derived neurotrophic factor (GDNF) to the brain has considerable potential as a neuroprotective strategy in Parkinson's disease (PD), its ability protect complex motor functions relevant human condition yet be established. In this study, we used an operant task that assesses selection, initiation and execution lateralized nose‐pokes Lister Hooded rats assess efficacy with which behaviours are protected against neurotoxic...
Ciliary neurotrophic factor (CNTF) is a potent protective for striatal neurons in animal models of Huntington9s disease (HD). Clinical application this potential therapeutic still requires the design and optimization delivery systems. In case HD, spatial spread vast volume occupied by striatum long-term are particular challenges these We explored adenovirus-mediated gene transfer to fulfill requirements studying functional anatomical effects single-site CNTF recombinant vectors rat model HD....
Our purpose was to investigate genes and molecular mechanisms involved in patients with Leber congenital amaurosis (LCA) model this type of LCA for drug screening. Fibroblasts from two unrelated clinically identified a yet undetermined gene mutation were reprogrammed pluripotency by retroviral transduction. These human induced pluripotent stem cells (hiPSCs) differentiated into neural (NSCs) that mimicked the tube stage retinal pigmented epithelial (RPE) could be targeted disease. A...
The retinal pigment epithelium (RPE), a multifunctional cell monolayer located at the back of eye, plays crucial role in survival and homeostasis photoreceptors. Dysfunction or death RPE cells leads to degeneration subsequent vision loss, such as Age-related macular some forms Retinitis Pigmentosa. Therefore, regenerative medicine that aims replace by new obtained from differentiation human pluripotent stem cells, is focus intensive research. However, despite their critical interest therapy,...
The outer blood-retinal barrier (oBRB) is compromised in several retinal pathologies, such as age-related macular degeneration affecting over 200 million people worldwide. This 200-350 μm thick tissue includes the pigment epithelium (RPE), Bruch's membrane, and vascularized choroid supplying retina. Degeneration of RPE and/or leads to photoreceptor loss and, ultimately, blindness. Current vitro co-culture oBRB models developed better understand diseases propose therapeutic alternatives are...
Within the framework of studies aiming at regenerative medicine for cardiovascular disease, we have developed an in vitro model to analyse human embryonic stem (ES) cell engraftment into myocardium. This is based on organotypic rat ventricular slices maintained culture air–medium interface semi-porous membranes. Survival and differentiation cardiomyocytes derived from ES cells were then assessed several months. In addition, observed that tissue not only exhibited normal histology, but also...
Abstract The molecular mechanisms underlying the heterogeneous effects of retinoic acid (RA) treatment on malignant glioma cells remain poorly understood. In this study, we present first evidence a functional role signal transduction factors (STATs) in RA‐induced proliferation, human glioblastoma GL‐15 cell line. We observed that STAT‐3 was constitutively activated and nuclei. then showed at low doses (0.01–1 μM) RA increased both proliferation rate phosphotyrosine (PY) activation STAT‐3....
Recent studies have significantly improved our ability to investigate cell transplantation and study the physiology of transplanted cells in cardiac tissue. Several previous shown that fully-immersed heart slices can be used for electrophysiological investigations. Additionally, ischemic induced by glucose oxygen deprivation offer a useful tool mechanical integration measure forces contraction engrafted cells, at least short term analysis. A recent novel model slices, prepared from rat human...