A5045866756- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Neuropeptides and Animal Physiology
- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Dietary Effects on Health
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Sleep and related disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Anesthesia and Sedative Agents
- Apelin-related biomedical research
- Nausea and vomiting management
- Ion Transport and Channel Regulation
- Sulfur Compounds in Biology
- Pain Management and Placebo Effect
Queen's University
2013-2023
University of Manitoba
2011
Queens University
2007-2010
Weatherford College
2008
Saint Louis University
2003-2007
The King's College
2007
Western General Hospital
1998
University of Edinburgh
1998
University of British Columbia Hospital
1988
Montreal General Hospital
1984-1985
We have examined potential functions of nitric oxide (NO) within the paraventricular nucleus (PVN) in urethan-anesthetized male Sprague-Dawley rats. Initial experiments demonstrated microinjection 50 pmol NO donor, sodium nitroprusside (SNP), directly into PVN resulted significant decreases mean blood pressure (BP) (-3,312 +/- 1,189 mmHg/s over 300-s response time; P < 0.05). To determine whether such effects were attributable to SNP-induced release, was administered by bilateral...
1. Whole-cell recordings were obtained from type I paraventricular nucleus (PVN) neurones in coronal slices of rat hypothalamus to study the involvement nitric oxide (NO) modulation inhibitory transmission resulting activation N-methyl-D-aspartate (NMDA) receptors by high affinity receptor agonist D,L-tetrazol-5-ylglycine. 2. A brief pulse NMDA (0.1-10 microM) faithfully elicited increases action potential firing frequency all cells tested (n = 55). In with membrane potentials positive -75...
Derived from the same prohormone, obestatin has been reported to exert effects on food intake that oppose those of ghrelin. The receptor GPR39 is present in brain and pituitary gland. Since gene encoding two peptides expressed also tissues, we examined further possible actions vivo vitro. Intracerebroventricular administration inhibited water drinking ad libitum-fed -watered rats, food-and water-deprived animals. preceded were more pronounced than any effect intake, did not appear be result...
In this study, we have utilized electrophysiological single unit recordings to evaluate the effects of nonpeptidergic angiotensin II (ANG II) antagonists on neural responses hypothalamic paraventricular nucleus (PVN) neurons either electrical stimulation in subfornical organ (SFO) or direct application ANG II. Electrical (200-400 microA; 0.1 ms) SFO resulted excitatory 36 50 PVN tested. Peristimulus histogram analysis such demonstrated latencies < 30 ms and variability response times...
Somatostatin is important in the regulation of diverse neuroendocrine functions. Based on bioinformatic analyses evolutionarily conserved sequences, we predicted another peptide hormone pro-somatostatin and named it neuronostatin. Immuno-affinity purification allowed sequencing an amidated neuronostatin 13 residues from porcine tissues. In vivo treatment with induced c-Fos expression gastrointestinal tissues, anterior pituitary, cerebellum, hippocampus. vitro promoted migration cerebellar...
The circumventricular organs (CVOs) are ideal locations at which circulating pyrogens may act to communicate with the CNS during an immune challenge. Their dense vasculature and fenestrated capillaries allow direct access of these tissue without impediment blood-brain barrier (BBB). One such CVO, subfornical organ (SFO), has been implicated as a site endogenous pyrogen interleukin 1beta (IL-1beta) acts initiate febrile response. This study was designed determine response rat SFO neurons...
Adiponectin is an adipocyte-derived peptide hormone involved in energy homeostasis and the pathogenesis of obesity, including hypertension. Area postrema (AP) lacks a blood–brain barrier critical homeostatic integration center for humoral neural signals. Here we investigate role AP adiponectin signaling. We show that rat expresses AdipoR1 AdipoR2 receptor mRNA. used current-clamp electrophysiology to whether influenced membrane properties neurons found ∼60% tested were sensitive adiponectin....
The mechanisms through which circulating ghrelin relays hunger signals to the CNS are not yet fully understood. In this study, we have examined potential role of subfornical organ (SFO), a circumventricular structure that lacks normal blood-brain barrier, as site in acts influence hypothalamic centers controlling food intake. We report increased intracellular calcium concentrations 28% (12 43) dissociated SFO neurons and expresses mRNA for growth hormone secretagogue receptor. Whole-cell...