A5033628217- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- COVID-19 diagnosis using AI
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Innovative Microfluidic and Catalytic Techniques Innovation
- Health, Environment, Cognitive Aging
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
University of Washington
2021-2025
A unique four-plexed RT-LAMP test kit operated by health workers can provide faster, more sensitive results than laboratory tests.
BackgroundDetection of SARS-CoV-2 infections is important for treatment, isolation infected and exposed individuals, contact tracing. RT-qPCR the "gold-standard" method to sensitively detect RNA, but most laboratory-developed assays involve complex steps. Here, we aimed simplify by streamlining reaction setup, eliminating RNA extraction, proposing reduced-cost detection workflows that avoid need expensive qPCR instruments.MethodA low-cost RT-PCR based "kit" was developed faster turnaround...
Point-of-care diagnostics often use isothermal nucleic acid amplification for qualitative detection of pathogens in low-resource healthcare settings but lack sufficient precision quantitative applications such as HIV viral load monitoring. Although (VL) monitoring is an essential component treatment, commercially available tests rely on relatively high-resource chemistries like real-time polymerase chain reaction and are thus used infrequent basis millions people living with low-income...
Rapid diagnostic tests (RDTs) for influenza used by individuals at home could potentially expand access to testing and reduce the impact of on health systems. Improving lead earlier diagnosis following symptom onset, allowing more rapid interventions those who test positive, including behavioral changes minimize spread. However, accuracy RDTs has not been determined in self-testing populations.This study aims assess an RDT conducted lay users with acute respiratory illness compared that a...
Abstract Background Early during the COVID-19 pandemic, it was important to better understand transmission dynamics of SARS-CoV-2, virus that causes COVID-19. Household contacts infected individuals are particularly at risk for infection, but delays in contact tracing, testing contacts, and isolation quarantine posed challenges accurately capturing secondary household cases. Methods In this study, 346 households Seattle region were provided with respiratory specimen collection kits remotely...
ABSTRACT RNA amplification tests allow sensitive detection of SARS-CoV-2 infection, but their complexity and cost are prohibitive for expanding COVID-19 testing. We developed “Harmony COVID-19”, a point-of-care test using inexpensive consumables, ready-to-use reagents, simple device that processes up to 4 samples simultaneously. Our lyophilized reverse-transcription, loop-mediated isothermal (RT-LAMP) can detect as little 15 copies per reaction, it report early 17 min with high viral load (2...
<sec> <title>BACKGROUND</title> Rapid diagnostic tests (RDTs) for influenza used by individuals at home could potentially expand access to testing and reduce the impact of on health systems. Improving lead earlier diagnosis following symptom onset, allowing more rapid interventions those who test positive, including behavioral changes minimize spread. However, accuracy RDTs has not been determined in self-testing populations. </sec> <title>OBJECTIVE</title> This study aims assess an RDT...