A5017070351- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Ovarian function and disorders
- Renal and related cancers
- DNA Repair Mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Effects and risks of endocrine disrupting chemicals
- Cancer Risks and Factors
- Genetic and phenotypic traits in livestock
- Carcinogens and Genotoxicity Assessment
- Molecular Biology Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Hair Growth and Disorders
- Kruppel-like factors research
- Heat shock proteins research
- Single-cell and spatial transcriptomics
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Mycotoxins in Agriculture and Food
Inner Mongolia University
2020-2025
Imperial College London
2013-2020
MRC London Institute of Medical Sciences
2020
Medical Research Council
2013-2016
The University of Tokyo
2010-2011
Highlights•Histone H3/H4 replacement is continuous and mediated by Hira during mouse oogenesis•Loss of results in chromatin abnormalities extensive oocyte loss•Hira depletion reduces histone load, which prevents normal transcriptional regulation•Hira-mediated required for 5mC deposition oocytesSummaryThe integrity chromatin, provides a dynamic template all DNA-related processes eukaryotes, maintained through replication-dependent -independent assembly pathways. To address the role absence...
Abstract Background Dynamic changes of histone posttranslational modifications are important contexts epigenetic reprograming after fertilization in pre-implantation embryos. Recently, lactylation has been reported as a novel modification that regulates various cellular processes, but its role during early embryogenesis not elucidated. Results We examined nuclear accumulation H3K23la, H3K18la and pan mouse oocytes embryos by immunofluorescence with specific antibodies. All the three were...
Histone H2A has several variants, and changes in chromatin composition associated with their replacement might involve structure remodeling. We examined the dynamics of canonical histone its three H2A.X, H2A.Z macroH2A, mouse during oogenesis pre-implantation development when genome remodeling occurs. Immunocytochemistry specific antibodies revealed that, although all variants were deposited nuclei full-grown oocytes, only H2A.X was abundant pronuclei one-cell embryos after fertilization,...
Sheep are an important livestock species whose gastrointestinal tract is essential for overall health. Feed contaminants such as bacterial toxins and mycotoxins severely damage the sheep intestine, yet mechanisms remain mostly elusive partially due to lack of physiologically relevant in vitro models. Here, we investigated molecular underlying deoxynivalenol (DON)-induced toxicity by developing intestinal organoids from isolated crypts Hu sheep. The had a central lumen monolayer epithelium,...
Phenanthrene (Phe), a typical low-molecular-weight polycyclic aromatic hydrocarbon (PAH) of three benzene rings, is one the most abundant PAHs detected in daily diets. Pregnant women and infants are at great risk Phe exposure. In present study, was administered to pregnant mice dose 0, 60, or 600 μg/kg body weight six times, F1 male showed significant reproductive disorders: testicular testis somatic index were significantly reduced; levels serum testosterone, GnRH SHBG increased, while FSH...
The genome of differentiated somatic nuclei is remodeled to a totipotent state when they are transplanted into enucleated oocytes. To clarify the mechanism this remodeling, we analyzed changes in composition core histone variants nuclear-transferred embryos, since recent evidence has revealed that chromatin structure can be as result variant replacement. We found donor cell-derived H3 H3.1, H3.2, and H3.3, well H2A H2A.Z, were rapidly eliminated from Accompanying removal, oocyte-stored H2A.X...
Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine and metabolic disorder in women, which characterized by androgen excess, ovulation dysfunction, polycystic ovary. Although the etiology of PCOS largely unknown, many studies suggest that aberrant DNA methylation an important contributing factor for its pathological changes. In this study, we investigated characteristics their impact on gene expression granulosa cells obtained from patients. Transcriptome analysis found...
5-Fluorouracil (5-FU) is a widely used chemotherapeutic agent whose incorporation into nucleic acid plays an essential role in its therapeutic efficacy. 5-FU induces severe reproductive toxicity, which has been shown to be reversible. However, the underlying mechanisms have not fully elucidated. Since single-strand-selective monofunctional uracil-DNA glycosylase 1 (Smug1) key enzyme excision of 5-FU, we investigated potential reversible toxicity by integrating knockdown, overexpression and...
Adipose tissue metabolism plays a crucial role in sheep meat quality and the optimization of adipose utilization. To reveal molecular mechanisms during growth naturally grazing sheep, we investigated mRNA miRNA profiles subcutaneous (SAT) from Sunit at 6, 18, 30 months age (Mth-6, Mth-18, Mth-30). We identified 927 differentially expressed (DE) genes 134 DE miRNAs SAT different stages. Specifically, expressions ACACA, FASN, DGAT2, GPAM, SCD, ELOVL6, HSD17B12, TECR, PKM, TKT, PCK1, CD44,...
The placenta is a vital organ that facilitates maternal-fetal circulation, ensuring proper fetal development. Phenanthrene (Phe), typical low-molecular-weight polycyclic aromatic hydrocarbon, widely present in the environment and food. In this study, pregnant mice were exposed to Phe (0, 0.6, 6, 60 μg/kg of body weight) via gavage every 3 days from pregnancy day 0.5 (PGD 0.5) for total six exposures during pregnancy. Placentas collected on PGD 18.5 analysis. results showed exposure altered...
Epidemiological investigations and animal studies demonstrate a significantly positive relationship between polycyclic aromatic hydrocarbons (PAHs) exposure reproductive disorders. However, few researches are focused on the toxicity of low-molecular-weight PAHs (number benzene ring ≤ 3) which occupy large part PAHs. Phenanthrene (Phe), typical PAH, is one most abundant detected in foods. In present study, oral treatment with Phe at human related level during gestation (60 μg/kg body weight...
Somatic cell nuclear transfer (SCNT), also known as somatic cloning, is a commonly used technique to study epigenetic reprogramming. Although SCNT has the advantages of being safe and able obtain pluripotent cells, early developmental arrest happens in most embryos. Overcoming barriers currently primary strategy for improving reprogramming efficiency rate In this study, we analyzed DNA methylation profiles vivo fertilized embryos with different fates. Overall level was higher during global...
Abstract Naïve pluripotency is a transient state during mammalian development that can be recapitulated indefinitely in vitro by inhibition of the mitogen-activated protein kinase (MAPK/Erk) signalling and activation STAT Wnt pathways. How Erk inhibited vivo to promote naïve remains largely unknown. By combining live cell imaging quantitative proteomics we found FGF2, known activator pro-differentiation cue, induces instead long-term both ES cells mouse embryos. We show results from...
Abstract The mule is the interspecific hybrid of horse and donkey has vigor in muscular endurance, disease resistance, longevity over its parents. Here, we examined adult fibroblasts (MAFs) compared with cells from their parents (donkey fibroblasts) (each species repeated three independent individuals) proliferation, apoptosis, glycolysis found significant differences. We subsequently derived mule, donkey, doxycycline (Dox)-independent induced pluripotent stem (miPSCs, diPSCs, hiPSCs)...
Histone chaperone FACT (facilitates chromatin transcription) is well known to promote recovery during transcription. However, the mechanism how regulates genome-wide accessibility and transcription factor binding has not been fully elucidated. Through loss-of-function studies, we show here that component Ssrp1 required for DNA replication damage repair also essential progression of cell phase transition proliferation in mouse embryonic fibroblast cells. On molecular level, absence leads...