Abstract Diabetes mellitus (DM) is characterized by elevated blood glucose and advanced glycation end product (AGEs) levels. Increased AGEs in bone tissue inhibit osteogenic differentiation marrow mesenchymal stem cells (BMSCs), leading to loss osteoporosis diabetic patients. Enhancing the capacity of BMSCs presence abundant can improve health prevent DM The flavonoid, Quercetin, has anti‐inflammatory, antibacterial, antitumor properties; however, it insoluble water thus not easily absorbed...

Bone defects caused by trauma, tumor resection, or developmental abnormalities are important issues in clinical practice. The vigorous development of tissue engineering technology provides new ideas and directions for regenerating bone defects. Hydroxyapatite (HAp), a bioactive ceramic, is extensively used because its excellent osteoinductive performance. However, application challenged single function conventional environment-unfriendly synthesis methods. In this study, we successfully...

Abstract Animal-derived biomaterials have been extensively employed in clinical practice owing to their compositional and structural similarities with those of human tissues organs, exhibiting good mechanical properties biocompatibility, extensive sources. However, there is an associated risk infection pathogenic microorganisms after the implantation from pigs, cattle, other mammals humans. Therefore, researchers begun explore development non-mammalian regenerative biomaterials. Among these...

Advanced glycation end products (AGEs) are considered a cause of diabetic osteoporosis. Although adipose-derived stem cells (ASCs) widely used in the research bone regeneration, mechanisms osteogenic differentiation ASCs from osteoporosis model remain unclear. This work aimed to investigate influence and molecular AGEs on potential ASCs.Enzyme-linked immunosorbent assay was measure change osteoporotic control C57BL/6 mice. were obtained inguinal fat AGEs, 5-aza2'-deoxycytidine (5-aza-dC)...

Abstract Objectives Bone marrow mesenchymal stem cells (BMSCs) hold a high osteogenic differentiation potential, but the mechanisms that control ability of BMSCs from osteoporosis (OP‐BMSCs) need further research. The purpose this experiment is to discuss effect Mettl3 on OP‐BMSCs and explore new therapeutic target can enhance bone formation OP‐BMSCs. Materials Methods bilateral ovariectomy (OVX) method was used establish SD rat OP model. Dot blots were reveal different methylation levels...

The significant clinical feature of bisphosphonate-related osteonecrosis the jaw (BRONJ) is exposure necrotic jaw. Other manifestations include pain, swelling, abscess, and skin fistula, which seriously affect patients' life, there no radical cure. Thus, new methods need to be found prevent occurrence BRONJ. Here, a novel nanoparticle, tFNA-KLT, was successfully synthesized by us, in nanoparticle tetrahedral framework nucleic acid (tFNA) used for carrying angiogenic peptide, KLT, then...

Abstract Objectives To study the accuracy of partially guided and fully templates applied to implant surgery anterior teeth. Materials methods Sixty patients who were scheduled receive dental treatment in region enrolled randomly assigned one following groups ( n = 20 each): routine implant‐supported restoration (control group, 30 implants), using a template (test group 1, 36 2, 33 implants). The depth was controlled for template. After implantation, planned implants placed superimposed...

Abstract Our previous finding revealed that the Wnt10b RNA expression of osteoporotic adipose‐derived stem cells (OP‐ASCs) with impaired osteogenic capacity was significantly reduced than ASCs. There are no ideas relationship between OP‐ASCs' potential and expression. This study aimed to indicate molecular mechanisms functional role in OP‐ASCs, as well investigate a application reverse differentiation potential. The OP‐ASCs ASCs were harvested from inguinal fat osteoporosis (OP) mice...

Abstract Objectives Diabetes mellitus‐related osteoporosis is caused by the imbalance between bone absorption and formation. Advanced glycation end products ( AGE s) are considered a cause of diabetic osteoporosis. Although adipose‐derived stem cells ASC promising adult in tissue regeneration, ability osteogenesis s environment needs to explore. This study aimed investigate influence on osteogenic potential explore signalling pathways involved its effect. Materials methods were isolated from...

Abstract Diabetes mellitus, characterized by abnormally high blood glucose levels, gives rise to impaired bone remodeling. In response (HG), the attenuated osteogenic differentiation capacity of human periodontal ligament stem cells (hPDLSCs) is associated with loss alveolar bone. Recently, DNA methylation was reported affect in pathological states. However, intrinsic mechanism linking ability presence HG still unclear. this study, we found that diabetic rats increased levels ligaments...

Abstract Adipose-derived stem cells (ASCs) from diabetic osteoporosis (DOP) mice showed impaired osteogenic differentiation capacity. Recent studies have shown that in addition to antidiabetic drugs, sodium-glucose co-transporter inhibitor-2 (SGLT-2), empagliflozin, can play multipotent roles through various mechanisms of action. In this study, we aimed investigate the effects and underlying empagliflozin on ASCs DOP mice. Our results potential autophagy activity weakened DOP-ASCs when...

Bone tissue engineering based on adipose-derived stem cells (ASCs) is expected to become a new treatment for diabetic osteoporosis (DOP) patients with bone defects. However, compared control ASCs (CON-ASCs), osteogenic potential of DOP-ASCs decreased, which increased the difficulty reconstruction in DOP patients. Moreover, cause poor osteogenesis hyperglycemic microenvironment has not been elucidated. Therefore, this study explored molecular mechanism decline from perspective epigenetics...

The guided tissue regeneration (GTR) technique is a promising treatment for periodontal defects. GTR membranes build mechanical barrier to control the ingrowth of gingival epithelium and provide appropriate space tissues, particularly alveolar bone. However, existing only serve as barriers lack biological activity induce bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using biomimetic mineralization method with sericin an organic...

Abstract Background Diabetes-related osteoporosis (DOP) is a chronic disease caused by the high glucose environment that induces metabolic disorder of osteocytes and osteoblast-associated mesenchymal stem cells. The processes bone defect repair regeneration become extremely difficult with DOP. Adipose-derived cells (ASCs), as seed in tissue engineering technology, provide promising therapeutic approach for DOP patients. osteogenic ability ASCs lower model than control ASCs. DNA methylation,...

Abstract Background Diabetic osteoporosis (DOP) is a systemic metabolic bone disease caused by diabetes mellitus (DM). Adipose-derived stem cells (ASCs) play an important role in regeneration. Our previous study confirmed that ASCs from DOP mice (DOP-ASCs) have lower osteogenesis potential compared with control (CON-ASCs). However, the cause of this poor has not been elucidated. Therefore, investigated underlying mechanism decline osteogenic DOP-ASCs perspective epigenetics and explored...

Abstract Background Osteoporosis (OP) is a metabolic bone disease involving reduced mass. Adipose‐derived stem cells (ASCs) play an important role in regeneration. Emerging evidence suggests that methyltransferase‐like 3 (METTL3) plays significant development and metabolism. Therefore, this study investigates changes to METTL3 the osteogenic differentiation of adipose osteoporotic rats (OP‐ASCs) explores ways enhance their ability. Methods An animal model osteoporosis was established by...

Background Head and neck squamous cell carcinoma (HNSCC) is the most common type of head cancer characterized by its aggressive nature variable prognosis response to immunotherapy. Tertiary lymphoid structures (TLSs) play crucial roles in creating a favourable immune microenvironment control tumour progression. However, specific impact these on HNSCC has not been thoroughly studied. Methods In this study, comprehensive review tertiary was conducted analysing 9 TLS-associated genes cohort 871...

ABSTRACT Human periodontal ligament stem cells (hPDLSCs) have emerged as promising candidates for the treatment of osteoporotic bone defects. Previous studies indicated that m 6 A plays a crucial role in regulating osteogenic differentiation hPDLSCs. However, research on relationship between YTHDC1, reading protein, and hPDLSCs remains unexplored. This study aimed to investigate biological roles YTHDC1 explore underlying mechanisms. Dot blot analysis revealed progressive increase methylation...