A5085787604- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Insect symbiosis and bacterial influences
- Crustacean biology and ecology
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Studies on Chitinases and Chitosanases
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Genetic and phenotypic traits in livestock
- Marine Bivalve and Aquaculture Studies
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Aquatic life and conservation
- Virus-based gene therapy research
- Genetic diversity and population structure
- Insect Resistance and Genetics
- Phytoplasmas and Hemiptera pathogens
- Genetics and Plant Breeding
- Fish Biology and Ecology Studies
University of Arizona
2017-2025
Mahidol University
2024
Chulalongkorn University
2024
Kasetsart University
2024
Asian Institute of Technology
2024
National Science and Technology Development Agency
2024
Chiang Mai University
2024
Ministry of Environment and Climate Change
2020
BrioBiotech (United States)
2013-2017
Advanced BioNutrition (United States)
2007-2010
ABSTRACT Pattern recognition proteins such as lipopolysaccharide and β-1,3-glucan binding protein (LGBP) play an important role in the innate immune response of crustaceans insects. Random sequencing cDNA clones from a hepatopancreas library white spot virus (WSV)-infected shrimp provided partial ( Ps EST-289) that showed similarity to LGBP gene crayfish Subsequently full-length was cloned by 5′-RACE (rapid amplification ends) technique sequenced. The is 1,352 bases length capable encoding...
Abstract Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay White Spot Syndrome Virus, most devastating virus impacting global shrimp aquaculture. Further, combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting create fully field-deployable, next-generation diagnostic with potential transform veterinary pathology,...
Abstract The emergence of antibiotic‐resistant bacteria (ARBs) and genes (ARGs) in aquaculture underscores the urgent need for alternative veterinary strategies to combat antimicrobial resistance (AMR). These measures are vital reduce likelihood entering a post‐antibiotic era. Identifying environmentally friendly biotechnological solutions prevent treat bacterial diseases is crucial sustainability minimizing use antimicrobials, especially antibiotics. development probiotics with...
ABSTRACT A rapid and highly sensitive real-time PCR detection quantification method for infectious hypodermal hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, white spot (WSV), double-stranded (dsDNA) infecting penaeid shrimp ( Penaeus sp.), was developed using the GeneAmp 5700 sequence system coupled with SYBR Green chemistry. The mixture contains fluorescence dye, Green, which upon binding to dsDNA exhibits enhancement. enhancement of proportional initial concentration...
Abstract The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp ( Penaeus spp.) aquaculture worldwide. To prevent diseases shrimp, the manipulation of gut microbiota has been suggested. However, prior knowledge host-microbiome necessary. We assessed modulation microbiome (bacteria/fungi) and its predicted functions over course disease progression experimentally challenged with EHP for 30 days using high throughput 16S...
White Feces Syndrome (WFS) is an emergent disease of penaeid shrimp ( Penaeus monodon and P . vannamei ) that identified by the presence floating white fecal strings on pond water in grow-out ponds. Although clinical manifestations WFS are well defined, underling etiology remains obscure. has been associated with several enteric pathogens, including Enterocytozoon hepatopenaei (EHP). The association based studies found areas where reported, prevalence severity EHP infection high. In this...
ABSTRACT For decades, untreated sewage flowing northward from Tijuana, Mexico, via the Tijuana River has adversely affected water quality of recreational beaches San Diego, California. We used quantitative reverse transcription-PCR to measure levels hepatitis A virus (HAV) and enteroviruses in coastal waters near United States-Mexico border compared these those conventional fecal indicators, Escherichia coli enterococci. Over a 2-year period 2003 2005, total 20 samples were assayed at two...
White spot disease, caused by infection with white syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of world through number previously identified mechanisms. ability to distinguish and trace strains WSSV great benefit identify, then limit, translocation routes disease. Here, we describe novel genotyping method using 34 short tandem repeat viral genome concurrently. This technique highly sensitive...
Abstract Background Enterocytozoon hepatopenaei (EHP) is an enteric pathogen that affects Penaeus vannamei and monodon shrimp in many SE Asian countries. In the western hemisphere, EHP was reported for first time 2016 farmed P. Venezuela. Anecdotal evidence suggests more prevalent grow-out ponds where salinity high (> 15 parts per thousand (ppt)) compared to with low salinities (< 5 ppt). Considering euryhaline species, we were interested knowing if can propagate salinities. Results...