Oroxylin A is a major active component of the Chinese traditional medicinal plant Scutellaria baicalensis Georgi, which has been reported as potential anticancer drug. We demonstrated that, inhibited glycolysis and binding hexokinase II (HK II) with mitochondria in human breast carcinoma cell lines, was dependent on sirtuin-3 (SIRT3). The level SIRT3 increased by A. Then deacetylated cyclophilin D, diminished its peptidyl-prolyl cis-trans isomerase activity induced dissociation from adenine...

Alterations of cellular metabolism play a central role in the development and progression cancer. Oroxylin A, an active flavonoid Chinese traditional medicinal plant, was previously shown to modulate glycolysis cancer cells. However, mechanism by which oroxylin A regulates is still not well defined. Here, we show that inhibits breast cells via Sirtuin 3 (SIRT3)-mediated destabilization hypoxia-inducible factor 1α (HIF1α), controls glycolytic gene expression. promotes superoxide dismutase...

p53 plays important roles in regulating the metabolic reprogramming of cancer, such as aerobic glycolysis. Oroxylin A is a natural active flavonoid with strong anticancer effects both vitro and vivo.wt-p53 (MCF-7 HCT116 cells) cancer cells p53-null H1299 were used. The glucose uptake lactate production analyzed using Lactic Acid Detection kit Amplex Red Glucose Assay Kit. Then, protein levels RNA p53, mouse double minute 2 (MDM2), p53-targeted glycolytic enzymes quantified Western blotting...

Oroxylin A is a flavonoid extracted from the root of Scutellaria baicalensis Georgi. Our previous research demonstrated that oroxylin have various anti-tumor effects including apoptosis, cell cycle arrest, drug-resistant reversion, and others. This paper explores mechanism how induce apoptosis by regulating uncoupling protein 2 (UCP2) in human colon cancer cells. We found inhibition UCP2 siRNA significantly increased sensitivity cells to drugs, reactive oxygen species (ROS) generation...

In this study, the anticancer effect of a newly synthesized flavonoid FV-429, against human breast cancer MDA-MB-231 cells, and underlying mechanisms were investigated. FV-429 triggered apoptosis simultaneously inhibited glycolysis cells. Both HK II activity its level in mitochondria significantly down regulated by FV-429. Moreover, weakened interaction between HKII VDAC, stimulated detachment from mitochondria, resulted opening mitochondrial permeability transition pores. Thus induced...

// Libin Wei 1 , Yuxin Zhou Jing Yao Chen Qiao Ting Ni Ruichen Guo 2 Qinglong and Na Lu State Key Laboratory of Natural Medicines, Jiangsu Carcinogenesis Intervention, China Pharmaceutical University, Nanjing, The People's Republic Xi′an Middle School Shanxi Province, Xi′an, Correspondence to: Guo, email: Lu, Keywords : lactate, HIF-1α, gluconeogenesis, inflammation, microenvironment Received October 25, 2014 Accepted March 20, 2015 Published April 15, Abstract Reprogramming energy...

Metabolic alteration in cancer cells is one of the most conspicuous characteristics that distinguish from normal cells. In this study, we investigated influence and signaling ways oroxylin A affecting cell energy metabolism under hypoxia. The data showed remarkably reduced generation lactate glucose uptake hypoxia HepG2 Moreover, inhibited HIF‐1α expression its stability. downstream targets (PDK1, LDHA, HK II), as well their mRNA levels were also suppressed by silencing or overexpression...

ABSTRACT Background Circulating cytokines were reported to be related diabetic nephropathy (DN) in observational studies. However, the causal relationship between them remains unknown. This study aimed investigate DN and circulating with genetic data frame of Mendelian Randomization (MR). Methods We performed a two‐sample MR analysis individuals European ancestry, utilizing publicly available genome‐wide association (GWAS) statistics. selected eligible instrumental SNPs that significantly...

Several studies have suggested that drug resistance in colon cancer patients with diabetes may be associated long-term insulin administration, which turn decreases the survival rate. Metformin is a commonly used to treat but has been recently demonstrated potential therapeutic effect on cancer. This study aimed elucidate underlying mechanism by metformin reverts insulin-induced oxaliplatin human HCT116 cells.Two cell lines (HCT116 and LoVo) were verify whether expression of receptor...

Abstract Recent studies have demonstrated that acquisition of cancer stem-like properties plays an essential role in promoting epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) resistance non-small cell lung (NSCLC); however, how to regulate and EGFR-TKI is largely unclear. In this study, we discovered increased iroquois-class homeodomain protein 4 (IRX4) was related gefitinib NSCLC cells. Knockdown IRX4 inhibited proliferation, sphere formation, the expression CD133,...

// Chen Qiao 1, * , Na Lu Yuxin Zhou 1 Ting Ni Yuanyuan Dai Zhiyu Li 2 Qinglong Guo Libin Wei State Key Laboratory of Natural Medicines, Jiangsu Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, People’s Republic Department Medicinal Chemistry, These authors contributed equally to this work Correspondence to: Guo, e-mail: anticancer_drug@163.com Wei, M.D, wlbiws_1986@aliyun.com Keywords: p53, translocation, oroxylin A, oxidative stress Received: August...

<b><i>Objective:</i></b> Diabetic cardiomyopathy (DCM) is a serious complication of type 2 diabetes mellitus (T2DM), resulting in unfavorable prognosis. Icariin (ICA) major flavonoid isolated from the traditional oriental herbal medicine <i>Epimedium</i> that has been recently proved to show potential therapeutic efficacy on T2DM. The aim this study was investigate underlying mechanism how ICA improved DCM rat models....

Myocardial hypertrophy is a chronic cardiac condition that often occurs from long-term pressure or volumetric load on the heart. Propranolol hydrochloride has been employed in research hypertension, pheochromocytoma, myocardial infarction, arrhythmias, angina pectoris, and hypertrophic cardiomyopathy. Current treatments for this have side effects, such as arrhythmias cell death, thus necessitating safer more effective alternatives. Recently, natural products gained attention drug development...

Diabetic nephropathy (DN) is the most common and serious complication of diabetes mellitus. Shionone (SH), an important triterpenoid compound in root extract Aster, might exert a protective effect DN mice high glucose cultivated glomerular podocytes. The current study aimed to unravel underlying mechanism by which SH mitigates DN. We postulate that stimulates expression sestrin-2 (SESN2), pivotal stress-inducible protein anti-inflammasome machinery.

Objective: This study measured the human epididymis protein 4 (HE4) and CA125 levels in Chinese women with benign gynecological disorders.Material methods: Sera were obtained from prior to surgery for a pelvic mass HE4 determined. The proportions of patients compared.Results: There 68 diseases. less elevated than (1% V.S. 29%, P<0.001). significant difference was observed endometriosis/endometriomas which not 53% (P<0.001). Serum level cystadenoma (0% 23%, P<0.001) germ cell tumors...