A5081417503- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Receptor Mechanisms and Signaling
- Enzyme Catalysis and Immobilization
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Protein Structure and Dynamics
- Machine Learning in Bioinformatics
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Proteomics Techniques and Applications
- Transgenic Plants and Applications
- Bioinformatics and Genomic Networks
- Cancer Research and Treatments
- Enzyme Structure and Function
- Biotin and Related Studies
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Protein purification and stability
- bioluminescence and chemiluminescence research
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- thermodynamics and calorimetric analyses
National Hellenic Research Foundation
2016-2025
Alexander Fleming Biomedical Sciences Research Center
2022-2025
The University of Texas at Austin
2007-2012
Princeton University
2005-2007
Technological Educational Institute of Athens
2005
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with severe socio-economic impact. A hallmark of ALS pathology the presence aberrant cytoplasmic inclusions composed misfolded and aggregated proteins, including both wild-type mutant forms. This review highlights critical role protein species in pathogenesis, particularly focusing on Cu/Zn superoxide dismutase (SOD1) TAR DNA-binding 43 (TDP-43), emphasizes urgent need for innovative therapeutic strategies targeting...
With the ultimate goal of identifying robust cellulases for industrial biocatalytic conversions, we have isolated and characterized a new thermostable very halotolerant GH5 cellulase. This enzyme, termed CelDZ1, was identified by bioinformatic analysis from genome polysaccharide-enrichment culture isolate, initiated material collected an Icelandic hot spring. Biochemical characterization CelDZ1 revealed that it is glycoside hydrolase with optimal activity at 70°C pH 5.0 exhibits good...
Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Here, we identified new esterolytic enzyme by screening metagenomic sample collected from hot spring Kamchatka, Russia. Biochemical characterization the esterase, termed EstDZ2, revealed that it is highly active medium chain fatty acid esters at temperatures between 25 and 60 °C pH values 7-8. The moderately thermostable with half-life more than six hours °C, but exhibits exquisite...
Inteins are the protein analogs of self-splicing RNA introns, as they post-translationally excise themselves from a variety hosts. Intein insertion abolishes, in general, activity its host protein, which is subsequently restored upon intein excision. These elements therefore have potential to be used general molecular "switches" for control arbitrary target proteins. Based on rational design, an intein-based switch has been constructed whose splicing conditionally triggered vivo by presence...
G protein-coupled receptors (GPCRs) are notoriously difficult to express, particularly in microbial systems. Using GPCR fusions with the green fluorescent protein (GFP), we conducted studies identify bacterial host effector genes that result a general and significant enhancement amount of membrane-integrated human GPCRs can be produced Escherichia coli. We show coexpression membrane-bound AAA+ protease FtsH greatly enhances expression yield four different class I GPCRs, irrespective presence...
ABSTRACT The efficient production of membrane proteins in bacteria remains a major challenge. In this work, we sought to identify overexpressed genes that enhance the yields recombinant Escherichia coli . We developed genetic selection system for bacterial protein production, consisting fusions with enzyme β-lactamase and facile high-production strains on ampicillin-containing media. This was used screen ASKA library, an ordered library plasmids encoding all known E. open reading frames...
Membrane proteins perform critical cellular functions in all living organisms and constitute major targets for drug discovery. Escherichia coli has been the most popular overexpression host membrane protein biochemical/structural studies. Bacterial production of recombinant proteins, however, is typically hampered by poor accumulation severe toxicity host, which leads to low final biomass minute volumetric yields. In this work, we aimed rewire E. protein-producing machinery withstand caused...
We report the preparation, characterization and application of a novel magnetic four-enzyme nanobiocatalyst prepared by simultaneous covalent co-immobilization cellulase (CelDZ1), β-glucosidase (bgl), glucose oxidase (GOx) horseradish peroxidase (HRP) onto surface amino-functionalized nanoparticles (MNPs). This was characterized various spectroscopic techniques. The process yielded maximum recovered enzymatic activity (CelDZ1: 42%, bgl: 66%, GOx: 94% HRP: 78%) at 10% v/v cross-linker...
Abstract Fibroblasts are key regulators of inflammation, fibrosis, and cancer. Targeting their activation in these complex diseases has emerged as a novel strategy to restore tissue homeostasis. Here, we present multidisciplinary lead discovery approach identify optimize small molecule inhibitors pathogenic fibroblast activation. The study encompasses medicinal chemistry, molecular phenotyping assays, chemoproteomics, bulk RNA‐sequencing analysis, target validation experiments, chemical...
Engineered protein-based sensors of ligand binding have emerged as attractive tools for the discovery therapeutic compounds through simple screening systems. We previously shown that engineered chimeric enzymes, which combine ligand-binding domains nuclear hormone receptors with a highly sensitive thymidylate synthase reporter, yield report presence hormone-like changes in bacterial growth. This work describes an optimized estrogen sensor Escherichia coli extraordinary reliability...
The overexpression of G protein-coupled receptors (GPCRs) and many other heterologous membrane proteins in simple microbial hosts, such as the bacterium Escherichia coli, often results protein mistargeting, aggregation into inclusion bodies or cytoplasmic degradation. Furthermore, production is very frequently accompanied by severe cell toxicity. In this work, we have employed a genetic strategy to isolate E. coli mutants that produce markedly increased amounts human central cannabinoid...
Xylanolytic enzymes have a broad range of applications in industrial biotechnology as biocatalytic components various processes and products, such food additives, bakery coffee extraction, agricultural silage functional foods. An increasing market demand has driven the growing interest for discovery xylanases with specific industrially relevant characteristics, stability at elevated temperatures presence other denaturing factors, which will facilitate their incorporation into processes. In...
Taking immediate action to combat the urgent threat of CO
Recent advances in computational biology have enabled solutions to the inverse folding problem - finding an amino acid sequence that folds into a target structure. An open question concerns design of proteins addition having correct structure also precisely tuned kinetic properties, such as and unfolding rates. To address this problem, we formulate quest for with free energy landscape. propose procedure here describe Inverse Folding Molecular Dynamics (IF-MD) method, which combines enhanced...
The vast majority of microbial diversity remains unculturable, limiting access to novel biotechnological resources. Advances in metagenomics have expanded the understanding communities, yet targeted protein discovery challenging. This study introduces ProteoSeeker, a command-line tool for streamlined metagenomic identification and annotation. ProteoSeeker operates two primary modes: i) Seek mode, which screens proteins according user-defined families, ii) Taxonomy uncovers taxonomy host...
Protein misfolding and aggregation are central features of a wide range diseases, including neurodegenerative disorders, systemic amyloidoses, cancer. The identification compounds that can modulate protein folding is key step toward developing effective therapies. High-throughput screening methods essential for efficiently identifying such compounds. In this study, we optimized previously developed high-throughput genetic screen monitoring in bacteria. This system based on the fluorescence...
The presence of terminal, surface-exposed sialic acid moieties can greatly enhance the in vivo half-life glycosylated biopharmaceuticals and improve their therapeutic efficacy. Complete homogeneous sialylation glycoproteins be efficiently performed enzymically vitro but this process requires large amounts catalytically active sialyltransferases. Furthermore, standard microbial hosts used for large-scale production recombinant enzymes only produce small quantities glycosyltransferases animal...
Metagenomic analysis of environmental samples provides deep insight into the enzymatic mixture corresponding niches, capable revealing peptide sequences with novel functional properties exploiting high performance next-generation sequencing (NGS) technologies. At same time due to their ever increasing complexity, there is a compelling need for larger computational configurations ensure proper bioinformatic analysis, and fine annotation. With aiming address challenges such an endeavor, we...
Membrane proteins (MPs) execute a wide variety of critical biological functions in all living organisms and constitute approximately half current targets for drug discovery. As the case soluble proteins, bacterium Escherichia coli has served as very popular overexpression host biochemical/structural studies membrane well. Bacterial recombinant protein production, however, is typically hampered by poor cellular accumulation severe toxicity host, which leads to low levels final biomass minute...