A5023203756- Reproductive Biology and Fertility
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Microtubule and mitosis dynamics
- Congenital heart defects research
- Animal Genetics and Reproduction
- Renal and related cancers
- Reproductive biology and impacts on aquatic species
University of Wisconsin–Madison
2014-2023
Laboratory of Molecular Genetics
2015
Maternal homozygosity for three independent mutant hecate alleles results in embryos with reduced expression of dorsal organizer genes and defects the formation dorsoanterior structures. A positional cloning approach identified all mutations as stop codons affecting same gene, revealing that encodes Glutamate receptor interacting protein 2a (Grip2a), a containing multiple PDZ domains known to interact membrane-associated factors including components Wnt signaling pathway. We find grip2a mRNA...
In zebrafish embryos, factors involved in both axis induction and primordial germ cell (PGC) development are localized to the vegetal pole of egg. However, upon egg activation experience an asymmetric off-center shift whereas PGC undergo symmetric animally-directed movement. We examined spatial relationship between proposed dorsal genes wnt8a grip2a factor dazl at cortex. find that RNAs for these localize different cortical depths, with RNA a deeper level than those axis-inducing factors....
ABSTRACT We show that the zebrafish maternal-effect mutation too much information (tmi) corresponds to prc1-like (prc1l), which encodes a member of MAP65/Ase1/PRC1 family microtubule-associated proteins. Embryos from tmi homozygous mutant mothers display cytokinesis defects in meiotic and mitotic divisions early embryo, indicating Prc1l has role midbody formation during cell division at egg-to-embryo transition. Unexpectedly, maternal function is also essential for reorganization vegetal...
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into developing oocyte. Because these rely on maternal which typically act very soon after fertilization-that preexist inside egg, standard approaches for expression and functional reduction involving injection reagents fertilized egg ineffective. Instead, such manipulations must be performed oogenesis, prior to or accumulation products. This...
Abstract We show that the zebrafish maternal-effect mutation too much information ( tmi ) corresponds to prc1-like prc1l ), which encodes a member of MAP65/Ase1/PRC1family microtubule-associated proteins. Embryos from tmi/prc1l homozygous mutant mothers display cytokinesis defects in meiotic and mitotic divisions early embryo, indicating has role midbody formation during cell division at egg-to-embryo transition. Unexpectedly, maternal function is also essential for reorganization vegetal...
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into developing oocyte. Because these rely on maternal which typically act very soon after fertilization-that preexist inside egg, standard approaches for expression and functional reduction involving injection reagents fertilized egg ineffective. Instead, such manipulations must be performed oogenesis, prior to or accumulation products. This...