A5001000854- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Otitis Media and Relapsing Polychondritis
- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Fungal Infections and Studies
- Immunodeficiency and Autoimmune Disorders
- Psoriasis: Treatment and Pathogenesis
- Plant Pathogens and Fungal Diseases
- RNA Interference and Gene Delivery
- Cutaneous lymphoproliferative disorders research
- Ubiquitin and proteasome pathways
- Eosinophilic Disorders and Syndromes
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- NF-κB Signaling Pathways
- interferon and immune responses
- Musculoskeletal synovial abnormalities and treatments
- Antifungal resistance and susceptibility
- Multiple Myeloma Research and Treatments
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
Center for Cancer Research
2023-2024
National Cancer Institute
2023-2024
National Cancer Institute
2023
Medical University of South Carolina
2019-2023
National Institutes of Health Clinical Center
2023
National Institutes of Health
2023
University of Alabama at Birmingham
2010-2015
Southern Illinois University School of Medicine
2009
Follicular regulatory T (Tfr) cells act as the counterpart of follicular helper (Tfh) to suppress germinal center (GC) B cell differentiation. We recently showed that interleukin-21 (IL-21) promoted Tfh differentiation in autoimmune BXD2 mice develop spontaneous GCs. This study was undertaken determine modulatory effects IL-21 on Tfr and balance mice.The percentage phenotype were determined BXD2-IL21(-/-) mice. The cell:Tfh ratio evaluated. Sorted from cocultured with cells, or transferred...
As an E2-conjugating enzyme for sumoylation, Ubc9 plays a critical role in sumoylation-mediated cellular pathways, ultimately impacting cell growth and cancer development. The aim of this study was to investigate the regulation cells.Immunohistochemistry Western blot were used determine expression paraffin-embedded tumor tissue frozen specimens matched tumors from same patient, respectively. To establish causal relationship between miR-30e expression, we overexpressed then determined...
Germinal centers (GCs) provide a microenvironment that promotes and regulates the interactions of B cells with follicular Th (TFH) cells. In this study, we show there are significantly higher frequencies CXCR5(+)ICOS(+) TFH in autoimmune BXD2 mice, these express both IL-21R IL-17RA. Although IL-17 IL-21 important for formation spontaneous GCs development pathogenic autoantibodies, IL-21, but not IL-17, is required proper mice. The total numbers their ability to induce cell responses vitro...
Regulator of G protein signaling (RGS) proteins inhibit chemokine by desensitizing protein-coupled receptor signals. This study was undertaken to determine the mechanisms which RGS13 promotes generation pathogenic autoantibodies in germinal centers (GCs), using BXD2-Rgs13-/- mice.
Objective Bidirectional interactions between granulocyte–macrophage colony‐stimulating factor–positive (GM‐CSF+) T cells and interferon regulatory factor 5–positive (IRF‐5+) macrophages play a major role in autoimmunity. In the absence of SH2 domain–containing phosphatase 1 (SHP‐1), GM‐CSF–stimulated are resistant to death receptor (DR)–mediated apoptosis. The objective this study was determine whether TRA‐8, an anti–DR5 agonistic antibody, can eliminate inflammatory CD4 SHP‐1–deficient...
Microascus gracilis is a specie of the genus in family Microascaceae and has been isolated from lung. It never reported as cause disseminated infection humans. Herein, we report fatal case 65-year-old man with history primary idiopathic pulmonary fibrosis, status-post bilateral lung transplant. His course was complicated by donor cultures positive for multiple organisms persistent pleural effusions. Multiple biopsy bronchial lavage specimens were negative mold. Later, fluid grew M. confirmed...
Nodular lymphocyte predominant Hodgkin lymphoma was termed "nodular B-cell lymphoma" in the International Consensus Classification (ICC), to emphasize clinical and biological differences from classic (CHL). The abbreviation "NLP" represents both terms ICC World Health Organization classifications. Variations growth pattern, originally reported as Fan patterns A-F, are designated either grade 1 or 2 ICC. NLP is uncommon, some cases an accurate diagnosis challenging. objectives of this article...
Abstract Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare but clinically aggressive hematologic malignancy, believed to originate from cells (PDCs) although it possesses multilineage potentials. Due the dismal prognosis, accurate and rapid diagnosis critical for early management. The disease usually initially involves skin bone marrow. Here we report cytopathologic findings in case of BPDCN involving liver patient previously diagnosed with lymph node biopsies. fine‐needle...
Abstract CXCR5+ follicular T helper (Tfh) cells locate in the light zone (LZ) of germinal centers (GCs) to help differentiation antibody producing B cells. Both IL-17 and IL-21 have been associated with development GCs but their effects on Tfh not clear. Autoimmune BXD2 mice developed spontaneous displayed increased number spleen. In BXD2-Il21-/- or BXD2-Il17ra-/- mice, there were significantly reduced PNA+Fas+ GC spleen autoantibody titers sera. This is exclusively related since percentage...
Abstract Follicular regulatory T cells (TFR) are a CD4 (Treg) subset that suppress germinal center (GC) B cell differentiation. Interleukin 21 (IL-21) promotes follicular helper (TFH) In the present study we investigated modulatory effects of IL-21 on TFR in autoimmune BXD2 mice develop spontaneous GCs with accumulation IL-21+TFH spleen. BXD2-Il21-/- mice, GL-7+Fas+GC and CXCR5+ICOS+TFHs were significantly reduced, but frequency was 2-fold higher than wild-type mice. Most CXCR5+ICOS+CD4...
Abstract Autoimmune BXD2 mice developed spontaneous germinal centers (GCs) and displayed increased number of CXCR5+ICOS+follicular T helper (Tfh) cells in the spleen. Although IL-21 but not IL-17 has been reported to be essential for development Tfh, there were significantly reduced PNA+Fas+GC B spleen BXD2-Il21-/- BXD2-Il17ra-/- mice. Interestingly, frequency Tfhs was 2.5-fold lower 1.5-fold higher compared wild type. increased, CXCR5+CD4 specifically localized GCs spleens. Administration...
Abstract Constitutively active NF-κB signaling with increased expression of p50 is associated many autoimmune diseases. In BXD2 mice, there dramatically higher in splenic B cells, compared to B6 cells (>10.0-fold, p<0.01). Surprisingly, both and p105 are significantly lower than those from BXD2-Il17ra-/- indicating IL-17 downregulates them cells. Consistent it, administration AdIL-17RA:Fc which blocks IL-17RA signaling, resulted decreased phospho-p65 leading autoantibody...
Abstract CXCR5+ follicular T helper (Tfh) cells primarily help B differentiate into antibody producing plasma and memory cells. The objective of this study is to determine how Tfh are involved in the pathogenic germinal center (GC) response autoimmune BXD2 mice. FACS analysis showed that there was 2.5 1.5 fold more ICOS+CXCR5+ IL21+CD4+ cells, respectively, spleens mice compared B6 Confocal microscopy examination revealed ICOS+CXCR5+CD4+ were mainly clustered GCs located adjacent CD21/CD35+...