A5035669505- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Nanopore and Nanochannel Transport Studies
- Immune Cell Function and Interaction
- Chemical Synthesis and Analysis
- Microfluidic and Capillary Electrophoresis Applications
- Genomics and Chromatin Dynamics
- Neural dynamics and brain function
- Immunotherapy and Immune Responses
- Cell death mechanisms and regulation
- Photoreceptor and optogenetics research
- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Biofuel production and bioconversion
- Medicinal Plant Pharmacodynamics Research
- Computational Drug Discovery Methods
- Neuroscience and Neural Engineering
- Neural Networks and Applications
- Gut microbiota and health
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
Harvard University
2013-2020
University of North Carolina at Chapel Hill
2011-2012
University of Chicago
2007-2008
Jackson Laboratory
2007
Simultaneously measuring the activities of all neurons in a mammalian brain at millisecond resolution is challenge beyond limits existing techniques neuroscience. Entirely new approaches may be required, motivating an analysis fundamental physical constraints on problem. We outline principles governing activity mapping using optical, electrical,magnetic resonance, and molecular modalities neural recording. Focusing mouse brain, we analyze scalability each method, concentrating limitations...
The accurate design of new protein-protein interactions is a longstanding goal computational protein design. However, most computationally designed interfaces fail to form experimentally. This investigation compares five previously described successful de novo interface designs with 158 failures. Both sets proteins were the molecular modeling program Rosetta. Designs considered success if high-resolution crystal structure complex closely matched model and equilibrium dissociation constant...
Significance Efficient cost-effective single-molecule sequencing platforms will facilitate deciphering complete genome sequences, determining haplotypes, and identifying alternatively spliced mRNAs. We demonstrate a nanopore-based by synthesis approach that accurately distinguishes four DNA bases electronically detecting differentiating different polymer tags attached to the terminal phosphate of nucleotides during their incorporation into growing strand in polymerase reaction. With nanopore...
Some protein design tasks cannot be modeled by the traditional single state strategy of finding a sequence that is optimal for fixed backbone. Such cases require multistate design, where threaded onto multiple backbones (states) and evaluated its strengths weaknesses on each For example, to can switch between two specific conformations, it necessary find compatible with both backbone conformations. We present in this paper generic implementation suited wide range demonstrate silico...
Computational design of novel protein–protein interfaces is a test our understanding protein interactions and has the potential to allow modification cellular physiology. Methods for designing high-affinity that adopt predetermined binding mode have proved elusive, suggesting need new strategies simplify process. A solvent-exposed backbone on β-strand thought as “sticky” pairing stabilizes many naturally occurring complexes. Here, we computationally redesign monomeric form symmetric...
Significance DNA sequencing has been dramatically expanding its scope in basic life science research and clinical medicine. Recently, a set of polymer-tagged nucleotides were shown to be viable substrates for replication electronically detectable nanopore. Here, we describe the design characterization polymerase–nanopore protein construct on an integrated chip. This system incorporates all four tagged distinguishes single–tagged-nucleotide addition real time. Coupling catalysis...
Abstract DNA polymerases have revolutionized the biotechnology field due to their ability precisely replicate stored genetic information. Screening variants of these enzymes for unique properties gives opportunity identify with novel features. We previously developed a single-molecule sequencing platform by coupling polymerase α-hemolysin pore on nanopore array. Here, we use this approach demonstrate method that enables rapid screening in multiplex manner. In approach, barcoded strands are...