- Microbial Fuel Cells and Bioremediation
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Water Treatment and Disinfection
- Ammonia Synthesis and Nitrogen Reduction
- Gut microbiota and health
- Metal Extraction and Bioleaching
- Anaerobic Digestion and Biogas Production
- Constructed Wetlands for Wastewater Treatment
- Fish Ecology and Management Studies
- Corrosion Behavior and Inhibition
- Hydrology and Sediment Transport Processes
- Carbohydrate Chemistry and Synthesis
- Environmental DNA in Biodiversity Studies
- Geochemistry and Elemental Analysis
- Heavy metals in environment
- Land Use and Ecosystem Services
- Coastal wetland ecosystem dynamics
- Geomagnetism and Paleomagnetism Studies
- Electrochemical sensors and biosensors
- Proteoglycans and glycosaminoglycans research
- Phosphorus and nutrient management
- Metalloenzymes and iron-sulfur proteins
- Electrochemical Analysis and Applications
- Glycosylation and Glycoproteins Research
National University of Singapore
2022-2025
Singapore Centre for Environmental Life Sciences Engineering
2022
The University of Queensland
2013-2020
Biological nitrogen removal through the nitrite pathway (NH4+ → NO2– N2) is favorable for wastewater treatment plants without sufficient carbon sources. This study demonstrates an innovative approach attaining based on sludge using free ammonia (FA, i.e., NH3). our discovery in this that FA at 210 mg NH3–N/L far less biocidal to ammonium-oxidizing bacteria (AOB) than nitrite-oxidizing (NOB). A total of 22% activated from sequencing batch reactor (SBR) receiving synthetic domestic was treated...
Abstract Stable nitritation is a critical bottleneck for achieving autotrophic nitrogen removal using the energy-saving mainstream deammonification process. Herein we report new strategy to wash out both Nitrospira sp. and Nitrobacter from treatment of domestic-strength wastewater. The combines sludge free nitrous acid (FNA) with dissolved oxygen (DO) control in reactor. Initially, nitrifying reactor achieved full conversion NH 4 + NO 3 − . Then, nitrite accumulation at ~60% was when 1/4...
Free nitrous acid (FNA) exerts a broad range of antimicrobial effects on bacteria, although susceptibility varies considerably among microorganisms. Among nitrifiers found in activated sludge wastewater treatment processes (WWTPs), nitrite-oxidizing bacteria (NOB) are more susceptible to FNA compared ammonia-oxidizing (AOB). This selective inhibition NOB over AOB WWTPs bypasses nitrate production and improves the efficiency costs nitrogen removal process both anaerobic ammonium oxidation...
An increase of nitrite in the domestic-strength range is generally recognized to stimulate nitrous oxide (N2O) production by ammonia-oxidizing bacteria (AOB). It was found this study, however, that N2O emission from a mainstream nitritation system (cyclic = 25–45 mg N/L) established free acid (FNA)-based sludge treatment not higher but much lower than initial nitrifying with full conversion NH4+-N NO3–-N. Under dissolved oxygen (DO) levels 2.5–3.0 mg/L, stage 76% stage. Even when DO level...
Interactions between microorganisms in mixed communities are highly complex, being either syntrophic, neutral, predatory, or competitive. Evolutionary changes can occur the interaction dynamics community members as they adapt to coexistence. Here, we report that syntrophic Geobacter sulfurreducens and Pseudomonas aeruginosa coculture change their over evolutionary time. Specifically, sp. dominance increases with adaptation within cocultures, determined through quantitative PCR fluorescence...
Nitrogen compounds need to be removed or captured from wastewater streams before disposal protect our aquatic environments eutrophication. Particular bacteria facilitating the biological removal of nitrogen during treatment include ammonia oxidising (AOB), nitrite (NOB), denitrifiers, as well anaerobic ammonium (Anammox) bacteria. Manipulating these microbial communities can improve efficiency in removal. Bypassing nitrate production by selectively inhibiting NOB reduces for oxygen and...