A5060178883- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- Animal Virus Infections Studies
- Epigenetics and DNA Methylation
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Fibroblast Growth Factor Research
- Kruppel-like factors research
- Lipid Membrane Structure and Behavior
Amgen (United States)
2021
Amgen (Canada)
2012-2017
Arbutus Biopharma (Canada)
2007-2009
Inimex Pharmaceuticals (Canada)
1999-2007
A monoclonal antibody mimic of FGF21 exerts beneficial metabolic effects in obese monkeys.
Single-cell RNA sequencing is a powerful tool to examine cellular heterogeneity, novel markers and target genes, therapeutic mechanisms in human cancers animal models. Here, we analyzed single-cell data of T cells obtained from multiple mouse tumor models by PCA-based subclustering coupled with TCR tracking using the STARTRAC algorithm. This approach revealed various differentiated cell subsets activation states, correspondence between tumors. analyses demonstrated peripheral that were...
Although it is well documented that the immunological activity of cytosine–guanine (CpG) motifs abrogated by 5′ methylation cytosine residue, encapsulation within stabilized lipid nanoparticles endows these methylated cytosine–guanine- (mCpG-) containing oligonucleotides (ODNs) with potent immunostimulatory in murine animal models. Surprisingly, not only do liposomal nanoparticulate (LN) mCpG ODN possess activity, their potency found to be equivalent and often greater than unmethylated form,...
A mixture of ionizable cationic lipids, steric barrier and colipids is used to encapsulate oligonucleotide DNA in lipidic particles called SALP. This material under development as an adjuvant for vaccines. Previously we have shown that coupling the antigen directly surface SALP can lead enhanced immunological responses vivo. Two different methods preparing ovalbumin−SALP were assessed this work. Originally conjugates prepared by treating containing a maleimide-derivatized lipid with...
The encapsulation of conventional drugs in lipid nanoparticles (LNs) has been extensively utilized to enhance therapeutic activity by altering their pharmacokinetic (PK) and biodistribution (BD) properties. We have previously shown that the immunostimulatory unmethylated cytidine-guanosine (CpG)-containing oligodeoxynucleotides (ODN) is greatly enhanced when encapsulated an LN (LN CpG-ODN). Here, we investigate effect circulation lifetime (determined composition) drug-to-lipid (D/L) ratio...
Lipid conjugates of oligo-(14-amino-3,6,9,12-tetraoxatetradecanoic acid) (ATTAn) were synthesized as monodisperse analogues poly(ethylene glycol) (PEG) derivatives used in liposomal drug delivery systems. The new lipids shown to be at least equivalent MePEGA-2000-DSPE assays designed evaluate the effectiveness polymers steric barrier molecules liposomes. Liposomes containing 1-5% ATTA8-DSPE (octamer) showed comparable long circulation behavior relative PEG-2000-DSPE analogues. Surprisingly,...