A5074615009- Environmental Toxicology and Ecotoxicology
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- Mass Spectrometry Techniques and Applications
- Identification and Quantification in Food
- Liver Disease Diagnosis and Treatment
- Gene expression and cancer classification
- Cancer-related gene regulation
- Head and Neck Cancer Studies
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
- Aquatic Invertebrate Ecology and Behavior
- Fish Ecology and Management Studies
- Environmental DNA in Biodiversity Studies
- Autophagy in Disease and Therapy
- Pharmaceutical and Antibiotic Environmental Impacts
- Trace Elements in Health
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Ferroptosis and cancer prognosis
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2025
Forum Réfugiés - Cosi
2020-2025
Centre Clermont-Auvergne-Rhône-Alpes
2020-2024
Centre international de recherche sur le cancer
2015-2021
Stress Environnementaux et Biosurveillance des Milieux Aquatiques
2019
Ospedale Bassini
1995-2018
Istituto Ramazzini
2005-2018
Assistance Publique – Hôpitaux de Paris
2009-2015
Hôpital Paul-Brousse
2009-2015
Inserm
2009-2014
The Cesare Maltoni Cancer Research Center of the European Ramazzini Foundation has conducted a long-term bioassay on aspartame (APM), widely used artificial sweetener. APM was administered with feed to 8-week-old Sprague-Dawley rats (100-150/sex/group), at concentrations 100,000, 50,000, 10,000, 2,000, 400, 80, or 0 ppm. treatment lasted until natural death, which time all deceased animals underwent complete necropsy. Histopathologic evaluation pathologic lesions and organs tissues collected...
In a previous study conducted at the Cesare Maltoni Cancer Research Center of European Ramazzini Foundation (CMCRC/ERF), we demonstrated for first time that aspartame (APM) is multipotent carcinogenic agent when various doses are administered with feed to Sprague-Dawley rats from 8 weeks age throughout life span.The aim this second better quantify risk APM, beginning treatment during fetal life.We studied groups 70-95 male and female APM (2,000, 400, or 0 ppm) 12th day until natural...
The liver is one of the richest organs in terms number and density mitochondria. Most chronic diseases are associated with accumulation damaged Hepatic mitochondria have unique features compared to other organs' mitochondria, since they hub that integrates hepatic metabolism carbohydrates, lipids proteins. Mitochondria also essential hepatocyte survival as mediator apoptosis necrosis. Hepatocytes developed different mechanisms keep mitochondrial integrity or prevent effects lesions,...
Functional characterization of long non-coding RNAs (lncRNAs) and their pathological relevance is still a challenging task. Abnormal expression few have been found associated with hepatocellular carcinoma, potential implications to both improve our understanding molecular mechanism liver carcinogenesis discover biomarkers for early diagnosis or therapy. However, the global role lncRNAs during HCC development in its infancy. In this study, we produced RNA-Seq data from 23 tissues (controls,...
Head and neck squamous cell carcinomas (HNSCCs) represent a heterogeneous group of cancers for which human papilloma virus (HPV) infection is an emerging risk factor. Previous studies showed promoter hypermethylation in HPV(+) oropharyngeal cancers, but only few consistent target genes have been so far described, the evidence functional impact on gene expression still limited. We performed global stratified pooled analyses epigenome-wide data HNSCCs based Illumina HumanMethylation450...
Whether ischemic preconditioning (IP) reduces ischemia/reperfusion (I/R) injury in human normal and fatty livers remains controversial. We compared two independent groups of liver donor transplants with versus without steatosis to evaluate IP consequences. Liver donors (n=22) or (n=28) either did not undergo before graft retrieval. Clinical data from the recipients, as well histological immunohistological characteristics post-reperfusion biopsies were analyzed. Incidence necrosis was...
Abstract Nonalcoholic steatosis is a liver pathology characterized by fat accumulation and severe metabolic alterations involving early mitochondrial impairment late hepatocyte cell death. However, dysfunction mechanisms remain elusive. Using four models of nonalcoholic steatosis, i.e., livers from patients with fatty disease, ob/ob mice, mice fed high-fat diet, in vitro lipotoxicity, we show that outer membrane permeability altered identified posttranslational modification voltage-dependent...
Hepatocellular carcinoma (HCC) is the second most common cause of cancer death worldwide, but still lacking sensitive and specific biomarkers for early diagnosis prognosis. In this study, we applied targeted massively parallel semiconductor sequencing to assess methylation on a panel genes (FBLN1, HINT2, LAMC1, LTBP1, LTBP2, PSMA2, PSMA7, PXDN, TGFB1, UBE2L3, VIM YWHAZ) in plasma circulating cell-free DNA (cfDNA) evaluate potential these as HCC two different series, one from France (42 cases...
Abstract The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) combination with an additional separation dimension ion mobility (IMS). high‐resolution trapped (TIMS) allowed efficient isobaric/isomeric showing distinct spatial...
Abstract The purpose of this study was to assess in rats the pharmacological parameters and effects on gene expression liver triterpene glycoside actein. Actein, an active component from herb black cohosh, has been shown inhibit proliferation human breast cancer cells. To conduct our assessment, we determined molecular actein livers Sprague‐Dawley treated with at 35.7 mg/kg for 6 24 h. Chemogenomic analyses indicated that elicited stress statin‐associated responses liver; altered cholesterol...
MicroRNAs (miRNAs) are small regulatory non-coding RNAs with a diversity of cellular functions, and frequently dysregulated in cancer. Using novel computational method (ActMir) that we recently developed, the "activity" miRNA hsa-miR-500a was implicated estrogen receptor (ER) positive breast cancer; however its targets functional impact remain poorly understood. Here, performed an extensive gene expression analysis ER+ cancer cell lines, to reveal miR-500a-5p after experimental modulation...
In Brief Background Ischemia-reperfusion (I/R) injury is the main cause of graft failure in liver transplantation (LT). Ischemic postconditioning (IPo) has shown to be beneficial against I/R injury. Our objective was compare results LT with or without IPo. Methods One hundred patients undergoing alternatively received IPo not. At time arterial reperfusion, consisted three 1-minute occlusions, interspersed reperfusion pauses. The primary endpoint postoperative aspartate aminotransferase (AST)...
The past decades have marked the rise of metabolomics and lipidomics as -omics sciences which reflect most phenotypes in living systems. Mass spectrometry-based approaches are acknowledged for both quantification identification molecular signatures, latter relying primarily on fragmentation spectra interpretation. However, high structural diversity biological small molecules poses a considerable challenge compound annotation. Feature-based networking (FBMN) combined with database searches...
Aspartame (APM) is one of the most widely used artificial sweeteners in world. Its ever-growing use more than 6000 products, such as soft drinks, chewing gum, candy, desserts, etc., has been accompanied by rising consumer concerns regarding its safety, particular potential long-term carcinogenic effects. In light inadequacy carcinogenicity bioassays performed 1970s and 1980s, a mega-experiment on APM was undertaken at Cesare Maltoni Cancer Research Center European Ramazzini Foundation groups...
Abstract Taking advantage of a large transcriptomic dataset recently obtained in the sentinel crustacean amphipod Gammarus fossarum , we developed an approach based on sequence similarity and phylogenetic reconstruction to identify key players involved endocrine regulation G . Our work identified three genes interest: nuclear receptors RXR E75, regulator broad-complex (BR). Their involvement molting reproduction, along with their sensitivity chemical contamination were experimentally...