A5039789844- Growth Hormone and Insulin-like Growth Factors
- MicroRNA in disease regulation
- RNA Research and Splicing
- Lipid metabolism and disorders
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Genetic Syndromes and Imprinting
- Tuberous Sclerosis Complex Research
- Sexual Differentiation and Disorders
- Paleontology and Stratigraphy of Fossils
- Epigenetics and DNA Methylation
- Polyomavirus and related diseases
- Radiomics and Machine Learning in Medical Imaging
- Geochemistry and Elemental Analysis
- Advanced X-ray and CT Imaging
- Genetic and Kidney Cyst Diseases
- Health Education and Validation
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Polyoxometalates: Synthesis and Applications
- Kruppel-like factors research
- RNA modifications and cancer
- Food Quality and Safety Studies
- Balance, Gait, and Falls Prevention
- Nutrition and Health Studies
Medical University of Lodz
2019-2024
Polish Mother’s Memorial Hospital Research Institute
2019-2021
AGH University of Krakow
2015-2017
Pasteurization of donated human milk preserves it for storage and makes safe feeding, but at the expense its composition, nutritional values functions. Here, we aimed to investigate impact Holder (HoP) High Pressure Processing (HPP) methods on miRNA in evaluate these changes Milk samples obtained from women 50th day lactation (n = 3) were subjected either HoP, HPP or remained unpasteurized as a control. Subsequently, was isolated whole material exosomal fraction sequenced with Illumina...
Abstract Identifying germline BRCA1/2 mutation carriers is vital for reducing their risk of breast and ovarian cancer. To derive a serum miRNA-based diagnostic test we used samples from 653 healthy women six international cohorts, including 350 (53.6%) with BRCA1 / 2 mutations 303 (46.4%) BRCA1/ wild-type. All individuals were cancer-free before at least 12 months after sampling. RNA-sequencing followed by differential expression analysis identified 19 miRNAs significantly associated BRCA...
Abstract Background The immune system has a central role in preventing carcinogenesis. Alteration of systemic cell levels may increase cancer risk. However, the extent to which common genetic variation influences blood traits and risk remains largely undetermined. Here, we identify pleiotropic variants predict their underlying molecular cellular alterations. Methods Multivariate Cox regression was used evaluate associations between diagnosis cases UK Biobank. Shared were identified from...
Abstract Background The consensus on how to choose a reference gene for serum or plasma miRNA expression qPCR studies has not been reached and none of the potential candidates have yet convincingly validated. We proposed new in silico approach finding suitable human, circulating miRNAs identified set endogenous based profiling experiments from Gene Expression Omnibus. used 3 known normalization algorithms (NormFinder, BestKeeper, GeNorm) calculate score. searched universal validated our...
Abstract Background Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms living organisms. Normalization is indispensable step of data analysis all those studies, since it removes unwanted, non-biological variability from data. In targeted qPCR typically performed with respect to prespecified reference genes, but the lack robust strategy their selection reported literature, especially studies concerning circulating microRNAs (miRNA). Unfortunately,...
Mathematical models have been applied in prediction of growth hormone treatment effectiveness children since the end 1990s. Usually they were multiple linear regression models; however, there are also examples derived by empirical non-linear methods. Proposed solution consists application machine learning technique - artificial neural networks to analyse this problem. This new methodology, contrary previous ones, allows detection both and dependencies without assuming their character a...
Vitamin D3 [25(OH)D] deficiency is a significant problem in Polish children. In many regions of the world, 25(OH)D concentrations show seasonal variation and differences between boys girls, due to insolation, as well different sociological cultural factors.The aim study was assess variations incidence vitamin D children from central Poland.The analysis comprised 1275 children, age 3-18 (11.2 ±4.0) years, with disorders growing and/or puberty, obesity, other complaints that could be related...
Introduction . The effectiveness of the currently utilized therapies for FoG is limited. Several studies demonstrated a beneficial impact Nordic walking (NW) on several gait parameters in Parkinson’s disease, but only one paper reported reduction freezing. Research Question In present study, question whether NW an effective therapeutic intervention FoG. Methods Twenty PD subjects trained 12 weeks, with frequency twice per week. Each session lasted about 60 minutes. patients control group did...
Pancreatic ductal adenocarcinoma (PDAC), due to its genomic heterogeneity and lack of effective treatment, despite decades intensive research, will become the second leading cause cancer-related deaths by 2030. Step-wise acquisition mutations, instability, is considered drive development PDAC; KRAS mutation occurs in 95 100% human PDAC, already detectable early premalignant lesions designated as pancreatic intraepithelial neoplasia (PanIN). This possibly key event instability PDAC...
Alström syndrome (ALMS) and Bardet–Biedl (BBS) are rare genetic diseases with a number of common clinical features ranging from early-childhood obesity retinal degeneration. ALMS BBS belong to the ciliopathies, which known have expression products genes, encoding them as cilia-localized proteins in multiple target organs. The aim this study was perform transcriptomic proteomic analysis on cellular models syndromes identify distinct pathological mechanisms present both syndromes. For purpose,...
In the majority of children with growth hormone (GH) deficiency (GHD), normal GH secretion may occur before attainment final height. The aim study was to assess incidence persistent and transient GHD effectiveness recombined human (rhGH) therapy in isolated idiopathic GHD, respect moment withdrawal according different diagnostic criteria GHD. Analysis included 260 patients (173 boys, 87 girls) who completed rhGH have reassessed IGF-1 secretion. pre- post-treatment compared together...
Childhood obesity, with its metabolic complications, is a problem of public health. The International Diabetes Federation (IDF) has recommended glucose levels 1 h post oral load (1h-PG) > 155–209 mg/dL as diagnostic for intermediate hyperglycemia (IH), while >209 type 2 diabetes (T2D). aim the study was to assess occurrence prediabetes, IH, and T2D in children adolescents simple obesity according criteria American Association (ADA) IDF, effect COVID-19 pandemic on these disorders....
Abstract In this paper, we presented the problem of predicting response to recombinant human growth hormone (GH) treatment in GH-deficient children. Such a prediction can be done by techniques mathematical modelling and is important because therapy consists daily injections expensive; thus, it should administered only those patients who will, with high probability, benefit from it. Until now, leading methodological approach was multiple regression analysis. Several authors demonstrated that...
State-of-art normal tissue complication probability (NTCP) models do not take into account more complex individual anatomical variations, which can be objectively quantitated and compared in radiomic analysis. The goal of this project was development NTCP model for radiation-induced hypothyroidism (RIHT) using imaging biomarkers (radiomics). We gathered CT images clinical data from 98 patients, who underwent intensity-modulated radiation therapy (IMRT) head neck cancers with a planned total...
Introduction.Prediction of recombinant human growth hormone (rhGH) therapy effectiveness seems an important issue, especially in situations when diagnosis is problematic.Until now the problem was solved by multiple regression analysis, while here we propose application artificial neural networks (ANN), particularly multilayer perceptron (MLP), as a novel tool.Material and methods.The analysis performed on data 289 patients, treated for at least 2 years due to GH deficiency.First,...
The aim of this study was to determine the serum profiles miRNAs in patients with tuberous sclerosis (TSC) upon sirolimus treatment and compare them those previously treated everolimus a similarly designed experiment. Serum microRNA profiling performed ten TSC before therapy again after 3–6 months using qPCR panels (Exiqon). Of 752 tested miRNAs, 28 showed significant differences expression between treatment. these, 11 were dysregulated same directions as groupcompared described group,...
Primary insulin-like growth factor-I (IGF-I) deficiency is a synonym of hormone (GH) insensitivity (GHI), however the necessity direct confirmation GH resistance by IGF-I generation test (IGF-GT) discussed. GHI may disturb intrauterine growth, nevertheless short children born small for gestational age (SGA) are treated with GH. We tested hypothesis that appropriate birth size (AGA), height standard deviation score (SDS) <−3.0, peak in stimulation tests (stimGH) ≥10.0 µg/L, <2.5...
Sarcomas are a heterogeneous group of malignant tumors, that develop from mesenchymal cells. tumors associated with poor prognosis and expected short overall survival. Efforts to improve treatment efficacy outcomes advanced metastatic sarcoma patients have not led significant improvements in the last decades. In Tp53C273X/C273X rat model we therefore aimed characterize specific gene expression pattern angiosarcomas loss TP53 function. The presence metabolically active several locations...
(1) Background: The aim of the present study was to assess cancer stem cell (CSC) markers CD24, CD44, CD133, and ALDH1A1 in rhabdomyosarcoma (RMS) children define their prognostic role this group patients. (2) Methods: material archival tissue specimens collected from 49 patients under 18 years age who had been diagnosed with RMS. Immunohistochemistry (IHC) used evaluate expression selected CSC tumor tissue. Expression evaluated using a semiquantitative IRS scale based on one developed by...
Saprochaete suaveolens is an ascomycetous yeast that produces a range of fruity flavors and fragrances. Here, we report the high-contiguity genome sequence ex-holotype strain, NRRL Y-17571 (CBS 152.25). The nuclear contains 24.4 Mbp codes for 8,119 predicted proteins.
Chromosome-scale genome assembly of the yeast Saprochaete ingens CBS 517.90 was determined by a combination technologies producing short (HiSeq X; Illumina) and long (MinION; Oxford Nanopore Technologies) reads. The 21.2-Mbp sequence has GC content 36.9% codes for 6,475 predicted proteins.
Abstract Motivation Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms living organisms. Normalization is indispensable step of data analysis all those studies, since it removes unwanted, non-biological variability from data. In targeted qPCR the normalization typically performed with respect to prespecified reference genes, but lack robust strategy their selection reported literature, especially studies concerning circulating microRNAs (miRNA)....
In the majority of children with growth hormone (GH) deficiency (GHD), normal GH secretion may occur before attainment final height. The aim study was to assess incidence persistent and transient GHD effectiveness recombined human (rhGH) therapy in isolated, idiopathic respect moment withdrawal according different diagnostic criteria GHD. analysis included 260 patients (173 boys, 87 girls) who had completed rhGH been reassessed for IGF-1 secretion. pre- post-treatment compared together...