A5102732840- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Silk-based biomaterials and applications
- CAR-T cell therapy research
- Telomeres, Telomerase, and Senescence
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Titanium Alloys Microstructure and Properties
- Mitochondrial Function and Pathology
- Biotin and Related Studies
- Liver physiology and pathology
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Advanced Proteomics Techniques and Applications
- MXene and MAX Phase Materials
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Analytical Chemistry and Chromatography
Xiamen University
2021-2023
Tianjin Medical University
2012-2022
Northwestern Polytechnical University
2022
Tianjin Economic-Technological Development Area
2022
Taiyuan University of Science and Technology
2022
Johns Hopkins University
2010
State University of New York
1993
An exosomal anchor peptide enables targeting, drug loading, and capture of exosomes from diverse origins targets oligonucleotides to muscle in mdx mice.
Hepatocellular carcinoma (HCC) remains a global challenge due to high morbidity and mortality rates poor response treatment. Immunotherapy, based on introduction of dendritic cells (DCs) activated by tumor cell lysates as antigens ex vivo , shows limited in HCC patients. Here, we demonstrate that cell–derived exosomes (TEXs), displaying an array antigens, can elicit stronger immune than vitro . Significant growth inhibition was achieved ectopic orthotopic mice treated with TEX‐pulsed DCs....
Abstract Treating large established tumors is challenging for dendritic cell (DC)-based immunotherapy. DC activation with tumor cell-derived exosomes (TEXs) carrying multiple tumor-associated antigen can enhance recognition. Adding a potent adjuvant, high mobility group nucleosome-binding protein 1 (HMGN1), boosts DCs’ ability to activate T cells and improves vaccine efficiency. Here, we demonstrate that TEXs painted the functional domain of HMGN1 (TEX-N1ND) via an exosomal anchor peptide...
Personalized immunotherapy utilizing cancer vaccines tailored to the tumors of individual patients holds promise for with high genetic heterogeneity, potentially enabling eradication tumor in its entirety.Here, we demonstrate a general strategy biological nanovaccines that trigger tumor-specific immune responses hepatocellular carcinoma (HCC). Dendritic cell (DC)-derived exosomes (DEX) are painted HCC-targeting peptide (P47-P), an α-fetoprotein epitope (AFP212-A2) and functional domain...
Antisense oligonucleotide (AO)–mediated splice correction therapy for Duchenne muscular dystrophy has shown huge promise from recent phase 2b clinical trials, however high doses and costs are required targeted delivery can lower both of these. We have previously demonstrated the feasibility AOs by conjugating a chimeric peptide, consisting muscle-specific peptide cell-penetrating to in mdx mice. Although increased uptake muscle was observed, majority peptide–AO conjugate found liver. To...
Abstract Carbohydrate-based infusion solutions are widely used in the clinic. Here we show that co-administration of phosphorodiamidate morpholino oligomers (PMOs) with glucose enhances exon-skipping activity Duchenne muscular dystrophy (DMD) mdx mice. We identify a glucose–fructose (GF) formulation potentiates PMO activity, completely corrects aberrant Dmd transcripts, restores dystrophin levels skeletal muscles and achieves functional rescue without detectable toxicity. This is attributed...
Early-activated CD8+ T cells increase both aerobic glycolysis and mitochondrial oxidative phosphorylation (OXPHOS). However, whether how the augmentation of OXPHOS regulates differentiation effector cell remains unclear. Here, we found that C1qbp was intrinsically required for such in antiviral antitumor immune responses. Activated C1qbp-deficient failed to respiratory capacities, resulting diminished acetyl–coenzyme A as well elevated fumarate 2-hydroxyglutarate. Consequently,...
Duchenne muscular dystrophy (DMD) is a devastating genetic disorder that leads to compromised cellular membranes, caused by the absence of membrane-bound dystrophin protein. Muscle membrane leakage results in disrupted intracellular homeostasis, protein degradation, and muscle wasting. Improving integrity may delay disease progression extend lifespan DMD patients. Here, we demonstrate exosomes, membranous extracellular vesicles, can elicit functional improvements dystrophic mice improving...
Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise for Duchenne muscular dystrophy (DMD) patients. However, recent failure with drisapersen, an AO candidate drug in phase 3 trial, highlights the importance of exploring other effective chemistries DMD. Previously, we demonstrated appreciable biological activity peptide nucleic acid (PNA) AOs restoring dystrophin expression dystrophin-deficient mdx mice intramuscularly. Here, further explore systemic...
Antisense oligonucleotide (AO)–mediated exon-skipping therapy is one of the most promising therapeutic strategies for Duchenne Muscular Dystrophy (DMD) and several AO chemistries have been rigorously investigated. In this report, we focused on effect 2′-O-methoxyethyl oligonucleotides (MOE) exon skipping in cultured mdx myoblasts mice. Efficient dose-dependent targeted 23 was achieved with MOE AOs different lengths backbone chemistries. Furthermore, established that 25-mer phosphorothioate...
Purpose: It is challenging to deliver the full-length dysferlin gene or protein restore cellular functions of dysferlin-deficient (DYSF -/-) myofibres in dysferlinopathy, a disease caused by absence dysferlin, which currently without effective treatment.Exosomes, efficient membranous nanoscale carriers biological cargoes, could be useful.Experimental design: Myotube-and human serum-derived exosomes were investigated for their capabilities restoring and murine DYSF -/-cells.Moreover,...
Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise in correcting frame-disrupting mutations the DMD gene for Duchenne muscular dystrophy. However, insufficient systemic delivery limits clinical adoption. Previously, we showed that a glucose/fructose mixture augmented AO to muscle mdx mice. Here, evaluated if fructose alone could enhance activities of AOs with different chemistries The results demonstrated improved potency tested greatest effect on...
Induction by hemin increases, while induction with 12-O-tetradecanoylphorbol-13-acetate (TPA) represses, erythroid-specific gene expression in the human cell line K562. We analyzed effects of or TPA on binding and activity transcription factors at a regulatory element found within transcriptional sequences many genes. increases ubiquitous AP-1 to this element. inhibits lineage limited factor NF-E2 control Hemin K562 cells does not facilitate its recognition site. appears nonspecifically...
Early detection and clear delineation of microscopic lesions during surgery are critical to the prognosis survival patients with hepatocellular carcinoma (HCC), a devastating malignancy without effective treatments except for resection. Tools specifically identify differentiate micronodules from normal tissue in HCC can have positive impact on survival. Here, we discovered peptide that preferentially binds cells through phage display. Significant accumulation fluorescence‐labeled tumor...
Mouse models are commonly used for studying hepatocellular carcinoma (HCC) biology and exploring new therapeutic interventions. Currently three main modalities of HCC mouse have been extensively employed in pre-clinical studies including chemically induced, transgenic transplantation models. Among them, preferred evaluating vivo drug efficacy settings given the short latency, uniformity size close resemblance to tumors patients. However methods establishing orthotopic diverse fragmentized...
Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription translation using an E. coli lysate system. Alternating selection solution immobilization microtiter wells used pan mRNA-ribosome-antibody (ARM) complexes. After seven rounds display, expression vector pTIG-TRX containing specific scFv DNAs transformed...
Approval of antisense oligonucleotide eteplirsen highlights the promise exon-skipping therapeutics for Duchenne muscular dystrophy patients. However, limited efficacy underscores importance to improve systemic delivery and efficacy. Recently, we demonstrated that a glucose fructose (GF) formulation effectively potentiates phosphorodiamidate morpholino oligomer (PMO). Considering clinical potential GF, it is important determine long-term compatibility with PMO in mdx mice prior translation....
It is a daunting therapeutic challenge to completely eradicate hepatocellular carcinoma (HCC) from patients. Alpha-fetoprotein (AFP) -based vaccines appear promising, however the efficacy needs be improved.