A5033950089- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Ocular Surface and Contact Lens
- Eicosanoids and Hypertension Pharmacology
- Advanced Glycation End Products research
- Tissue Engineering and Regenerative Medicine
- Microplastics and Plastic Pollution
- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Mesenchymal stem cell research
- Liver Disease Diagnosis and Treatment
- Silk-based biomaterials and applications
- Telomeres, Telomerase, and Senescence
- Biochemical and Structural Characterization
- Advanced Fluorescence Microscopy Techniques
- Bone and Dental Protein Studies
- Alcohol Consumption and Health Effects
- Corneal surgery and disorders
- Protein Structure and Dynamics
- 3D Printing in Biomedical Research
- Cholesterol and Lipid Metabolism
Shantou University
2010-2025
Chinese University of Hong Kong
2016-2025
Tsinghua University
2023-2024
Qingdao Center of Resource Chemistry and New Materials
2004
National Tsing Hua University
1998
To determine the expressions of SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) genes in human mouse ocular cells comparison to other tissue cells. Human conjunctiva primary pterygium tissues were collected from patients who underwent surgery. The expression ACE2 TMPRSS2 was determined conjunctival cells, cell lines, mesenchymal stem as well by reverse transcription-polymerase chain reaction (RT-PCR) SYBR green PCR. RT-PCR...
Additive manufacturing (AM) holds great potential for processing natural polymer hydrogels into 3D scaffolds exploitable tissue engineering and in vitro modelling. The aim of this research activity was to assess the suitability computer-aided wet-spinning (CAWS) AM hyaluronic acid (HA)/chitosan (Cs) polyelectrolyte complex (PEC) hydrogels. A post-printing treatment based on HA chemical cross-linking via transesterification with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) investigated...
Deep neural network-based programs can be applied to protein structure modeling by inputting amino acid sequences. Here, we aimed evaluate the AlphaFold2-modeled myocilin wild-type and variant structures compare experimentally determined structures. Molecular dynamic ligand binding properties of were also analyzed. showed high similarities in overall mutant structures, but orientations geometries side chains slightly different. The olfactomedin-like domain modeled missense fewer folding...
Purpose: to determine the metabolomics profiles in plasma samples of primary open-angle glaucoma (POAG) patients. Methods: The from 20 POAG patients under intraocular pressure (IOP)-lowering medication treatment and control subjects were subjected untargeted analysis, among which 10 further oxylipin-targeted analysis by liquid chromatography–mass spectrometry analysis. prediction accuracy differentially abundant metabolites was assessed receiver operating characteristic curves. Pathway...
To investigate the plasma lipoprotein subclasses in patients with primary open-angle glaucoma (POAG), a total of 20 Chinese POAG on intraocular pressure (IOP)-lowering treatment and age-matched control subjects were recruited. Based levels cholesterol (TC) low-density (LDL-C), study divided into elevated- normal-level subgroups. The lipoprotein, subclasses, oxidized LDL (oxLDL) quantitatively measured. discrimination potential lipoproteins was evaluated using area under receiver operating...
Abstract Tono-Pen and TonoVet have been used in rabbits to measure intraocular pressure (IOP) investigate the effect of IOP lowering therapies. Therefore, their reliability accuracy are very important deserve careful evaluation. Our results showed that with-subject deviation ( S w ) intraclass correlation coefficient (ICC) were 0.61 mmHg/0.83 mmHg 0.97/0.94, respectively for intrasession repeatability. For intersession reproducibility, Sw ICC 1.42 mmHg/1.66 0.73/0.67, respectively....
PURPOSE: To investigate the oxylipin profiles in aqueous humor of primary open angle glaucoma (POAG) patients. METHODS: Aqueous samples were collected from 17 POAG patients and 15 cataract subjects subjected to liquid chromatography-mass spectrometry (LC-MS) analysis detect oxylipins. The prediction potential differential abundant oxylipins was assessed by receiver operating characteristic (ROC) curves. Pathway correlation analyses on clinical biochemical parameters also conducted. RESULTS:...
Polyhydroxyalkanoate (PHA) synthesis regulatory protein PhaR contains a DNA binding domain (DBD) and PHA granule (GBD), it anchors to the promoter region of granule-associated (PhaP) repress phaP expression. However, will bind PHB granules be released from when are formed in vivo, initiating expression gene. Based on this mechanism, bacterial two-hybrid system was developed: separated into two parts: DBD used fuse with bait, GBD prey, replaced by reporter gene lacZ. expressed vivo inclusion...
Abstract Aims Diabetic retinopathy (DR) is a significant global public health concern. Alternative, safe, and cost‐effective pharmacologic approaches are warranted. We aimed to investigate the therapeutic potential of nattokinase (NK) for early DR underlying molecular mechanism. Methods A mouse model diabetes induced by streptozotocin was utilized NK administered via intravitreal injection. Microvascular abnormities were evaluated examining leakage from blood–retinal barrier dysfunction loss...
Abstract Primary open‐angle glaucoma (POAG) is the most common type of glaucoma. Using whole‐exome sequencing, we identified two independent families diagnosed as POAG from China with a novel EFEMP1 variant (Exon3, c.175A>C p.Met59Leu); Three previously reported variants c.1160G>A p.R387Q, c.1189T>C p.Y397H, and c.1429C>T p.R477C in EFEPM1 55 sporadic individuals were also identified. The p.Met59Leu co‐segregated disease phenotype within families. Immunoprecipitation western blot...
Retinal disease treatment by stem cell-based replacement relies on cell differentiation into retinal cells. We previously demonstrated that human periodontal ligament-derived cells can be directed lineage upon induction. Here, we report the transdifferentiation potential of adipose-derived (ASCs) and its enhancement Notch signaling modulation. Human ASCs, isolated from abdominal fat, expressed mesenchymal but not hematopoietic markers, they differentiate adipocytes, chondrocytes, osteoblasts...
Long-chain fatty acids (LCFAs) are one of the main energy-supplying substances in body. LCFAs with different lengths and saturations may have contrasting biological effects that exacerbate or alleviate progress against a variety systemic disorders lipid metabolism organisms. Nonalcoholic liver disease is characterized by chronic inflammation steatosis, mainly caused ectopic accumulation lipids liver, especially LCFAs. CD36 scavenger receptor recognizes mediates transmembrane absorption...
Investigate the oxylipin profiles in aqueous humor of primary open-angle glaucoma (POAG) patients.
The aim of the study is to establish an online database for predicting protein structures altered in ocular diseases by Alphafold2 and RoseTTAFold algorithms. Totally, 726 genes multiple were collected structure prediction. Both algorithms built locally using open-source codebases. A dataset with 48 from Protein Data Bank (PDB) was adopted algorithm set-up validation. website match corresponding predicted tertiary each amino acid sequence. local distance difference test-Cα (pLDDT) template...
Non-adherent culture is critical for the transdifferentiation of stem cells from mesoderm to neuroectoderm. Sphere has been reported directly induce adipose tissue neural cells. Here we aimed evaluate continuous non-adherent on potential human adipose-derived (ASCs) into retinal lineage. Human ASCs were induced lineage by treatment noggin, dickkopf-related protein 1, and IGF-1 (NDI) under adherent culture. The NDI induction with 21 days promoted robust expression markers in as compared those...
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Additive manufacturing (AM) holds great potential for processing natural polymer hydrogels into 3D scaffolds exploitable tissue engineering and in vitro modelling. The aim of this research activity was to assess the suitability computer-aided wet-spinning (CAWS) AM hyaluronic acid (HA)/chitosan (Cs) polyelectrolyte complex (PEC) hydrogels. A post-printing treatment based on HA chemical cross-linking via transesterification with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) investigated...