A5078344691- Cervical Cancer and HPV Research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Animal Genetics and Reproduction
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Respiratory viral infections research
- Reproductive System and Pregnancy
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- Proteoglycans and glycosaminoglycans research
- Oral microbiology and periodontitis research
- Cancer and Skin Lesions
- Viral Infectious Diseases and Gene Expression in Insects
- Oral Health Pathology and Treatment
- IL-33, ST2, and ILC Pathways
- Animal Disease Management and Epidemiology
- Nanowire Synthesis and Applications
- Prostate Cancer Treatment and Research
- T-cell and B-cell Immunology
- Nerve injury and regeneration
- Skin and Cellular Biology Research
Heidelberg University
2013-2024
German Cancer Research Center
2015-2024
DKFZ-ZMBH Alliance
1994-2023
Motamed Cancer Institute
2019
Academic Center for Education, Culture and Research
2019
National Center for Tumor Diseases
2018
Heidelberg University
2009
Hospital Santiago Apostol
2009
European Molecular Biology Laboratory
1995
European Molecular Biology Laboratory
1995
A synthetic E7 gene of human papillomavirus (HPV) type 16 was generated that consists entirely preferred codons. Expression analysis the in and animal cells showed levels protein 20- to 100-fold higher than those obtained with wild-type E7. Enhanced expression resulted from highly efficient translation, as well increased stability mRNA due its codon optimization. Higher transfected correlated significant loss cell viability various lines. In contrast, lower driven by a slight induction...
Abstract A characteristic neuropathological feature of Alzheimer's disease is the cerebral deposition amyloid plaques. These deposits contain b̃A4 peptide, a cleavage product transmembrane protein precursor (APP). Despite numerous studies on processing different APP isoforms in non‐neuronal cells, little known about its sorting and transport neurons cf central nervous system (CNS). To analyze this question we expressed cultured rat hippocampal human 695, tagged at N‐terminus with myc...
Abstract Background Cervical cancer is the second most common in women worldwide. NK and cytotoxic T cells play an important role elimination of virus-infected tumor through NKG2D activating receptors, which can promote lysis target by binding to major histocompatibility complex class I-related chain A (MICA) proteins. Increased serum levels MICA have been found patients with epithelial tumors. The aim this study was compare soluble (sMICA) NKG2D-expressing blood samples from cervical or...
Recent studies have provided evidence of T cell reactivity to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in significant numbers non-infected individuals, which has been attributed cross-reactive CD4 memory cells from previous exposure seasonal coronaviruses. Less CD8 documented date.We used the NetCTLPan neural network Epitope Database and Analysis Resource select a series 27 HLA-A*02:01 epitopes derived proteome SARS-CoV-2. Their binding capacity was assessed by...
To investigate the frequencies and bacterial load of three species periodontal bacteria in samples from oropharyngeal cancer patients versus healthy individuals.This is a case-control study based on biopsies collected tumor tissues obtained with squamous cell carcinoma between 2016 2017 shed oral mucosal epithelial cells that were controls using Cepimax® brush, carrying out several brushings towards posterior third edge tongue cheek. Porphyromonas gingivalis, Tannerella forsythia Prevotella...
Mutated proteins arising from somatic mutations in tumors are promising targets for cancer immunotherapy. They represent true tumor-specific antigens (TSAs) as they exclusively expressed tumors, reduce the risk of autoimmunity and more likely to overcome tolerance compared wild-type (wt) sequences. Hence, we designed a panel long peptides (LPs, 28–35 aa) comprising driver gene TP35 KRAS frequently found gastrointestinal test their combined immunotherapeutic potential. We increased numbers T...
The E6 and E7 early genes of human papillomavirus type 16 have been shown in vitro to play a central role the transforming capability this virus. To explore their effects on differentiating epithelial cells vivo, we used bovine cytokeratin 10 (K10) promoter target expression suprabasal layers epidermis transgenic mice. In two different lines mice efficiently expressing transgene, animals displayed generalized epidermal hyperplasia, hyperkeratosis parakeratosis skin forestomach, both known be...
The envelope glycoproteins of Semliki Forest virus (SFV), Vesicular Stomatitis (VSV), and Influenza Fowl Plague (FPV) are vectorially targeted in neurons to the plasma membrane dendrites (SFV VSV) axons (FPV). To gain insight into mechanisms responsible for such polarized delivery we have examined effects on nocodazole brefeldin A (BFA), which known cause microtubule depolymerization disassembly Golgi apparatus, respectively. Nocodazole treatment blocked transport all viral both dendrites....
Cervical cancers transformed by high risk human papilloma virus (HPV) express the E7 oncoprotein, which accelerates degradation of retinoblastoma protein (Rb). Here we show that E7-mediated Rb requires calcium-activated cysteine protease, calpain. bound and activated μ-calpain promoted cleavage at Rb810, with mutation this residue preventing degradation. The calpain product, Rb1–810, was unable to mediate cell cycle arrest but retained ability repress E6/E7 transcription. also accelerated...
The human helper-dependent adeno-associated virus type 2 (AAV-2) has been shown to induce differentiation in various cell types culture including pluripotent embryonic cells, the absence of helper virus. To assess whether induction may influence developmental processes we analysed effect AAV- on developing mouse embryos. In vitro infection fertilized eggs induced arrest development at twocell stage. Moreover, micro injection AAV-2 DNA (comprising either complete genome or a fragment...
Induction of effective immune responses may help prevent cancer progression. Tumor-specific antigens, such as those human papillomaviruses involved in cervical cancer, are targets with limited intrinsic immunogenicity. Here we show that immunization low doses (10(6) infectious units/dose) a recombinant adenovirus type 5 encoding fusion the E7 oncoprotein papillomavirus 16 to carboxyl terminus surface antigen hepatitis B virus (HBsAg) induces remarkable E7-specific humoral and cellular...
Cervical cancer and its precursor intra-epithelial lesions are linked to infection by a subset of so-called "highrisk" human papillomavirus types, which estimated infect nearly four hundred million women worldwide. Two prophylactic vaccines have been commercialized recently targeting HPV16 18, the most prevalent viral types found in cervical cancer, operate through induction capsid-specific neutralizing antibodies. However, patients with persistent these not protect against progression...
Abstract The human papillomavirus type 16 (HPV16) causes a large fraction of genital and oropharyngeal carcinomas. To maintain the transformed state, tumor cells must continuously synthesize E6 E7 viral oncoproteins, which makes them tumor‐specific antigens. Indeed, specific T cell responses against have been well documented CD8 + engineered to express receptors (TCRs) that recognize epitopes or tested in clinical studies with promising results, yet limited success. Using from peripheral...
Eradication of cancer cells by the immune system requires extravasation, infiltration and progression through tumor extracellular matrix (ECM). These are also critical determinants for successful adoptive cell immunotherapy solid tumors. Together with structural proteins, such as collagens fibronectin, heparan sulfate (HS) proteoglycans major components ECM. Heparanase 1 (HPSE) is only enzyme known to have endoglycosidase activity that degrades HS. HPSE expressed at high levels in almost all...
Natural killer (NK) cells are an important resource of the innate immune system directly involved in spontaneous recognition and lysis virus-infected tumor cells. An exquisite balance inhibitory activating receptors tightly controls NK cell activity. At present, one best-characterized is NKG2D, which promotes NK-mediated target by binding to a family surface ligands encoded MHC class I chain-related (MIC) genes, among others. The goal this study was describe expression pattern MICA MICB at...
Abstract Background The NKG2D receptor confers important activating signals to NK cells via ligands expressed during cellular stress and viral infection. This has generated great interest because not only is it on cells, but also seen in virtually all CD8 + cytotoxic T classically considered absent CD4 cells. However, recent studies have identified a distinctive population of that do express NKG2D, which could represent particular effector involved infections chronic diseases. On the other...