A5049230956- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Aquaculture disease management and microbiota
- Developmental Biology and Gene Regulation
- Congenital Heart Disease Studies
- Epigenetics and DNA Methylation
- Blood disorders and treatments
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- interferon and immune responses
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Underwater Vehicles and Communication Systems
- Mesenchymal stem cell research
- Invertebrate Immune Response Mechanisms
- Wind Turbine Control Systems
- Acute Myeloid Leukemia Research
- Coronary Artery Anomalies
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
Harvard University
2004-2017
Massachusetts General Hospital
2013-2017
Harvard Stem Cell Institute
2007-2013
Howard Hughes Medical Institute
2004-2007
Dana-Farber Cancer Institute
2007
Boston Children's Hospital
2004-2006
Brigham and Women's Hospital
2005
Second heart field (SHF) progenitors perform essential functions during mammalian cardiogenesis. We recently identified a population of cardiac progenitor cells (CPCs) in zebrafish expressing latent TGFβ-binding protein 3 (ltbp3) that exhibits several defining characteristics the anterior SHF mammals. However, ltbp3 transcripts are conspicuously absent lateral plate mesoderm (ALPM), where specified higher vertebrates. Instead, expression initiates at arterial pole developing tube. Because...
Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations the zebrafish moonshine (mon) gene specifically disrupt both embryonic adult hematopoiesis, resulting severe red blood cell aplasia. We report mon encodes ortholog of mammalian transcriptional intermediary factor 1gamma (TIF1gamma) (or TRIM33), a member TIF1 family During development, hematopoietic progenitor cells mutants fail...
The vertebrate heart forms through successive phases of cardiomyocyte differentiation. Initially, cardiomyocytes derived from first field (FHF) progenitors assemble the linear tube. Thereafter, second (SHF) differentiate into that are accreted to poles tube over a well-defined developmental window. Although elongation deficiencies lead life-threatening congenital defects, variables controlling initiation, rate and duration myocardial accretion remain obscure. Here, we demonstrate AP-1...
During mammalian embryogenesis, cardiac progenitor cells constituting the second heart field (SHF) give rise to right ventricle and primitive outflow tract (OFT). In zebrafish, previous lineage-tracing mutant analyses suggested that SHF ventricular OFT progenitors co-migrate arterial pole of zebrafish tube soon after their specification in nkx2.5+ anterior lateral plate mesoderm (ALPM). Using additional prospective lineage tracing, we demonstrate while migrate directly pole, become...