To determine whether parents of Very Low Birth Weight (VLBW) infants in the Neonatal Intensive Care Unit (NICU) transitioning home with NICU-2-Home smartphone application have greater parenting self-efficacy, are better prepared for discharge and shorter length stay (LOS) than control parents. A four-week pilot randomized controlled trial during transition 90 VLBW to usual care (n = 44) or plus 46), a designed Parenting Sense Competence Scale (PSOC) was assessed at baseline, day after...

Abstract Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) plays an important role in RNA processing via m 6 A modification of pre-mRNA or pre-miRNA. However, the functional and relationship between hnRNPA2/B1 early embryonic development are unclear. Here, we found that is crucial for by virtue regulating specific gene transcripts. HnRNPA2/B1 was localized to nucleus cytoplasm during subsequent development, starting at fertilization. Knockdown delayed after 4-cell stage blocked...

Efficient and precise genetic engineering in livestock such as cattle holds great promise agriculture biomedicine. However, techniques that generate pluripotent stem cells, well reliable tools for gene targeting livestock, are still inefficient, thus not routinely used. Here, we report highly efficient the bovine genome using cells clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 nuclease. First, induced (iPSCs) from somatic fibroblasts by ectopic expression of yamanaka...

This study examines qualitatively the concerns and coping mechanisms of fathers mothers very low-birth-weight (VLBW; <1500 g) neonatal intensive care unit (NICU) infants as they transition to home from NICU. In-depth, semistructured phone interviews were conducted with a sample VLBW NICU in Chicago area who had transitioned home, parental examined during home. Phone lasting 30 60 minutes transcribed verbatim, all coded using content narrative analysis. Twenty-five parents (10 fathers, 15...

The effects of organic osmolytes on cytoplasmic maturation porcine oocytes were examined in medium (modified Whitten′s medium) containing various NaCI concentrations. presence osmolytes, such as taurine and sorbitol, at 6 12 mM 68.49 or 92.40 increased oocyte glutathione content. Microfilament organization was disrupted the higher level (92.40 mM). However, supplementation with sorbitol to reduced severity abnormality. Early embryonic development vitro blastocyst stage 8.3 ± 0.9% for matured...

Although parents of premature infants experience many challenges when transitioning home from the neonatal intensive care unit, healthcare providers and social support systems tend to focus on mothers rather than fathers. Unfortunately, very little is known about paternal concerns needs as compared with maternal ones. The lack understanding may lead inadequate designs unit family programs less involved fathers, all which contribute increased burdens poor health outcomes for their infants....

During oocyte meiotic maturation, spindles form in the central cytoplasm and then migrate to cortex extrude a small polar body, forming highly polarized cell through process involving actin actin-related molecules. The mechanisms underlying polarization are still unclear. Arp2/3 complex regulates but it is not known whether WASP family of proteins, regulator complex, involved this context. In present study, role member WAVE2 mouse asymmetric division was investigated. (1) mRNA protein were...

Oocyte quality is crucial for successful fertilization and subsequent embryonic development. Post-ovulatory aging leads to reduced oocyte impaired embryogenesis, representing an unavoidable challenge in terms of certain assisted reproductive techniques. Diosmetin (DIOS), a natural flavonoid found lemons, spearmint, spider moss, exhibits antioxidant, anti-inflammatory, anti-apoptotic properties. However, its effects on the mature porcine oocytes vitro remain unexplored. This study...

In this study we imaged integral changes in microfilament assembly and cortical granule distribution, examined effects of inhibitor on the distribution during oocyte maturation, parthenogenetic activation vitro fertilisation pig. The were with fluorescent-labelled lectin rhodamine-labelled phalloidin under laser scanning confocal microscopy. At germinal vesicle stage, organelles located around cell cortex present as a relatively wide area oolemma. Microfilaments also observed uniform cortex....

Bilateral quadriceps tendon rupture is an unusual injury, but may be encountered in patients with various chronic diseases after minor trauma. This article presents a case of bilateral 38-year-old woman renal failure. Surgical repair was performed using bone tunnel technique nonabsorbable suture and anchor. Postoperative magnetic resonance imaging confirmed complete healing the site, clinically active extension 120 degrees range motion achieved.

We demonstrated normal fertilization processes (as determined by pronuclear formation, apposition and syngamy) in porcine oocytes either following intracytoplasmic spermatozoon (ICSI) or isolated sperm head injection. Microtubule organization chromatin configuration were investigated these during the first cell cycle. Following ICSI, microtubular aster was organized from neck of filled whole cytoplasm. These male-derived microtubules appear to move both pronuclei center oocytes. cytoskeletal...

Coxsackie virus and adenovirus receptor (CXADR) is a member of the immunoglobulin superfamily as well junctional adhesion molecule family receptor. In human pre-implantation embryos, CXADR was detected co-localized with tight junction (TJ) proteins on membrane trophectoderm. However, its physiological roles were not elucidated in terms blastocyst formation. Here, we reported expression patterns biological functions porcine embryos. The transcripts at all stages pre-implantation....

Wedelolactone (WDL) is a coumaryl ether compound extracted from the traditional Chinese medicinal plant, Eclipta prostrata L. It natural polyphenol that exhibits variety of pharmacological activities, such as anti-inflammatory, anti-free radical, and antioxidant activities in bone, brain, ovary. However, its effect on embryonic development remains unknown. The present study explored influence WDL supplementation porcine oocytes culture vitro underlying mechanisms levels Kelch-like...

Parabens are widely used in various industries, which including chemical, pharmaceutical, food, cosmetic, and plastic processing industries. Among these, methyl paraben (MP) serves as an antimicrobial preservative processed foods, pharmaceuticals, cosmetics, it is particularly detected baby care products. Studies indicate that MP functions endocrine-disrupting compound with estrogenic properties, negatively affecting mitochondrial bioenergetics antioxidant activity testicular germ cells....

We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression NCCIT cells. GDF3 was affected shRNA‐mediated downregulation exogenous overexpression of specifically, as well retinoic acid‐mediated differentiation. transcription activated NANOG, the upstream region (−183 to −1) sufficient induce minimal transcriptional activity. Moreover, binds promoter vivo activity is mediated domain. This study provides first evidence that a activator oncogenic...

Summary The objective of this study was to determine developmental pattern and cell allocation the inner mass trophectoderm in haploid diploid embryos following parthenogenetic activation. In vitro matured porcine oocytes were activated by ethanol treatment cultured presence or absence cytochalasin B for 5h. then NCSU23 9 days. combined with did not increase ( p &gt;0.1) incidence development blastocyst stage higher &lt;0.05) treatments as compared alone. percentage two female pronuclei &lt;...