A5072515548- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Phytoplasmas and Hemiptera pathogens
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Cellular transport and secretion
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
Institut National de la Recherche Scientifique
2015-2019
Biologie du Fruit et Pathologie
2017
Université de Bordeaux
2017
Armand Frappier Museum
2015
Turnip mosaic virus (TuMV) reorganizes the endomembrane system of infected cell to generate endoplasmic-reticulum–derived motile vesicles containing viral replication complexes. The membrane-associated protein 6K2 plays a key role in formation these vesicles. Using confocal microscopy, we observed that this protein, marker for complexes, localized extracellular space Nicotiana benthamiana leaves. Previously, showed RNA is associated with multivesicular bodies (MVBs). Here, using transmission...
Positive-sense RNA viruses remodel host cell endomembranes to generate quasi-organelles known as "viral factories" coordinate diverse viral processes, such genome translation and replication. It is also becoming clear that enclosing (vRNA) complexes within membranous structures important for virus cell-to-cell spread throughout the host. In plant cells infected by turnip mosaic (TuMV), a member of family Potyviridae, peripheral motile endoplasmic reticulum (ER)-derived vesicles are produced...
Plant viruses move systemically in plants through the phloem. They as virions or ribonucleic protein complexes, although it is not clear what these complexes are made of. The approximately 10-kb RNA genome of Turnip mosaic virus (TuMV) encodes a membrane protein, known 6K2, that induces endomembrane rearrangements for formation viral replication factories. These factories take form vesicles contain (vRNA) and proteins. In this study, we report presence 6K2-tagged containing vRNA...
Infection of plant cells by RNA viruses leads to the generation organelle-like subcellular structures that contain viral replication complex. During Turnip mosaic virus (TuMV) infection Nicotiana benthamiana, membrane protein 6K2 plays a key role in release motile vesicles from host endoplasmic reticulum (ER). Here, we demonstrate contains GxxxG motif within its predicted transmembrane domain is vital for TuMV infection. Replacement Gly with Val this inhibited production, and was due...