A5073895723- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Porphyrin Metabolism and Disorders
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Metalloenzymes and iron-sulfur proteins
- Biochemical and Structural Characterization
- Folate and B Vitamins Research
- Microbial metabolism and enzyme function
- biodegradable polymer synthesis and properties
- Polysaccharides and Plant Cell Walls
- Silk-based biomaterials and applications
- Microbial Natural Products and Biosynthesis
Jiangnan University
2016-2025
Jiangsu Industry Technology Research Institute
2025
State Key Laboratory of Food Science and Technology
2025
Thermal Power Research Institute
2024
Ministry of Education of the People's Republic of China
2022
The Synergetic Innovation Center for Advanced Materials
2013-2017
Second Affiliated Hospital of Jilin University
2017
Hebei University of Engineering
2016
Genentech
2012
State Key Laboratory of Microbial Technology
2008-2011
High-yield biosynthesis of hyaluronan (HA) with controllable molecular weights (MWs) remains challenging due to the poorly understood function Class I HA synthase (HAS) and metabolic imbalance between cellular growth. Here, we systematically characterize HAS identify crucial regions involved in polymerization, secretion, MW control. We construct mutants that achieve complete secretion expand range from 300 1400 kDa. By dynamically regulating UDP-glucose 6-dehydrogenase activity applying an...
Abstract 5-Aminolevulinic acid (ALA), the committed intermediate of heme biosynthesis pathway, shows significant promise for cancer treatment. Here, we identified that in addition to hemA and hemL , hemB hemD hemF hemG hemH are also major regulatory targets pathway. Interestingly, up-regulation benefited ALA accumulation whereas overexpression diminished accumulation. Accordingly, by combinatorial with different copy-number plasmids, titer was improved 3.25 g l −1 . Furthermore, combination...
Abstract Hyaluronan is widely used in cosmetics and pharmaceutics. Development of robust safe cell factories cultivation approaches to efficiently produce hyaluronan many interests. Here, we describe the metabolic engineering Corynebacterium glutamicum application a fermentation strategy manufacture with different molecular weights. C. engineered by combinatorial overexpression type I synthase, enzymes intermediate pathways attenuation extracellular polysaccharide biosynthesis. The strain...
Production of bioengineered heparin with recombinant yeast cells.
Poly-beta-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported the first time stress-induced system enabled E. coli, non-PHB producer, to mobilize PHB vivo by mimicking natural accumulation bacteria.The successful expression of biosynthesis and depolymerase genes coli was confirmed production 3-hydroxybutyrate secretion. Starvation experiment demonstrated complete served an...
Hyaluronidases (HAases), particularly leech HAases, have attracted intense attention due to their broad applications in medical treatments and great potential for the enzymatic production of hyaluronan oligosaccharides. However, little is known about this third interesting family HAases. Here, we applied random amplification cDNA ends polymerase chain reaction (RACE-PCR) approach identify first HAase-encoding gene. By combining protein engineering high-density culture, achieved high-level...
Characterization of genetic circuits and biosynthetic pathways in different hosts always requires promoter substitution redesigning. Here, a strong, broad-spectrum promoter, Pbs, for Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae was constructed, it incorporated into the minimal E. coli–B. subtilis–S. shuttle plasmid pEBS (5.8 kb). By applying random mutation strategy, three promoters Pbs1, Pbs2, Pbs3, with strengths were generated characterized. These will expand synthetic...
Establishment of a sustainable non-animal biosynthesis chondroitin sulfate from methanol using genetically engineered <italic>Pichia pastoris.</italic>
Cuprizone inhibits mitochondrial function and induces demyelination in the corpus callosum, which resembles pattern III lesions multiple sclerosis patients. However, molecular cellular mechanism by cuprizone remains unclear. Interleukin-17 (IL-17) secreted T helper 17 cells γδT are essential development of experimental autoimmune encephalomyelitis. In this study, we examined importance IL-17 signaling cuprizone-induced demyelination. We found that mice deficient IL-17A, receptor C (IL-17RC),...
Sulfonation as one of the most important modification reactions in nature is essential for many biological macromolecules to function. Development green sulfonate group donor regeneration systems efficiently compounds interest always attractive. Here, we design and engineer two different boost biosynthesis sulfated compounds. First, assemble three modules construct a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) system demonstrate its applicability living cells. After discovering adenosine...