A5052724489- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Multiple Myeloma Research and Treatments
- Kruppel-like factors research
- Biosimilars and Bioanalytical Methods
- Wnt/β-catenin signaling in development and cancer
- Metal Extraction and Bioleaching
- Cancer Genomics and Diagnostics
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Mine drainage and remediation techniques
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Chemokine receptors and signaling
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Signaling Pathways in Disease
- Lymphoma Diagnosis and Treatment
- Cardiac Fibrosis and Remodeling
- Bioinformatics and Genomic Networks
- Protein Tyrosine Phosphatases
Universidad de Navarra
2018-2025
Clinica Universidad de Navarra
2023-2025
Navarre Institute of Health Research
2019-2025
Universitat de Barcelona
2024
Hospital Clínic de Barcelona
2024
Centro de Investigación Biomédica en Red de Cáncer
2019-2024
Centro de Astrobiología
2008-2017
Consejo Superior de Investigaciones Científicas
2015
Instituto Nacional de Técnica Aeroespacial
2011-2014
Universidad Autónoma de Madrid
2008
Background: Cardiac fibroblasts (CFs) have a central role in the ventricular remodeling process associated with different types of fibrosis. Recent studies shown that do not respond homogeneously to heart injury. Because limited set bona fide fibroblast markers, proper characterization population heterogeneity response cardiac damage is lacking. The purpose this study was define CF during and underlying mechanisms regulate function. Methods: Collagen1α1–GFP (green fluorescent...
Identification of new markers associated with long-term efficacy in patients treated CAR T cells is a current medical need, particularly diseases such as multiple myeloma. In this study, we address the impact density on functionality BCMA cells. Functional and transcriptional studies demonstrate that high expression construct show an increased tonic signaling up-regulation exhaustion vitro cytotoxicity but decrease vivo BM infiltration. Characterization gene regulatory networks using...
Inflammation is a common feature in neurodegenerative diseases that contributes to neuronal loss. Previously, we demonstrated the basal inflammatory tone differed between brain regions and, consequently, reaction generated pro-inflammatory stimulus was different. In this study, assessed innate immune midbrain and striatum using an experimental model of Parkinson's disease. An adeno-associated virus serotype 9 expressing α-synuclein mCherry genes or gene administered into substantia nigra....
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. Aging myeloid malignant transformation are characterized by changes the composition regulation of HSPCs. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize an enriched population human HSPCs obtained from young elderly healthy individuals. Based on their transcriptional profile, identified proportions compartments...
Discovery of Fe-carbonate precipitation in Rio Tinto, a shallow river with very acidic waters, situated Huelva, South-western Spain, adds new dimension to our understanding carbonate formation. Sediment samples from this low-pH system indicate that carbonates are formed physico-chemical conditions ranging acid neutral pH. Evidence for microbial mediation is observed secondary electron images (Fig. 1), which reveal rod-shaped bacteria embedded the surface siderite nanocrystals. The formation...
Multiple myeloma (MM) is an incurable disease, whose clinical heterogeneity makes its management challenging, highlighting the need for biological features to guide improved therapies. Deregulation of specific long non-coding RNAs (lncRNAs) has been shown in MM, nevertheless, complete lncRNA transcriptome not yet elucidated. In this work, we identified 40,511 novel lncRNAs MM samples. accounted 82% and were more heterogeneously expressed than coding genes. A total 10,351 overexpressed 9,535...
Inflammation is a critical process for the progression of neuronal death in neurodegenerative disorders. Microglia play central role neuroinflammation and may affect neuron vulnerability. Next generation sequencing has shown molecular heterogeneity microglial cells; however, variability their response to pathological inputs remains unknown.To determine effect an inflammatory stimulus on cells, lipopolysaccharide (LPS) was administered peripherally mice status cortex, hippocampus, midbrain,...
Salar de Uyuni (SdU), with a geological history that reflects 50 000 years of climate change, is the largest hypersaline salt flat on Earth and estimated to be biggest lithium reservoir in world. Its salinity reaches saturation levels for NaCl, kosmotropic salt, high concentrations MgCL2 LiCl, both salts considered important chaotrophic stressors. In addition, extreme temperatures, anoxic conditions, UV irradiance, albedo extremely low phosphorous, make SdU unique natural environment which...
Abstract While myelodysplastic syndromes with del(5q) (del(5q) MDS) comprises a well-defined hematological subgroup, the molecular basis underlying its origin remains unknown. Using single cell RNA-seq (scRNA-seq) on CD34 + progenitors from MDS patients, we have identified cells harboring deletion, characterizing transcriptional impact of this genetic insult disease pathogenesis and treatment response. Interestingly, both non-del(5q) present similar lesions, indicating that all cells, not...
Myelodysplastic syndromes (MDS) are hematopoietic stem cell (HSC) malignancies characterized by ineffective hematopoiesis, with increased incidence in older individuals. Here we analyze the transcriptome of human HSCs purified from young and healthy adults, as well MDS patients, identifying transcriptional alterations following different patterns expression. While aging-associated lesions seem to predispose myeloid transformation, disease-specific may trigger development. Among MDS-specific...
Despite the potential of CAR-T therapies for hematological malignancies, their efficacy in patients with relapse and refractory Acute Myeloid Leukemia has been limited. The aim our study to develop manufacture a cell product that addresses some current limitations. We initially compared phenotype T cells from AML healthy young elderly controls. This analysis showed displayed predominantly effector phenotype, increased expression activation (CD69 HLA-DR) exhaustion markers (PD1 LAG3),...
Generating organs from stem cells through blastocyst complementation is a promising approach to meet the clinical need for transplants. In order generate rejection-free organs, of both parenchymal and vascular must be achieved, as endothelial play key role in graft rejection. Here, we used lineage-specific cell ablation system produce mouse embryos unable form cardiac systems. By intraspecies complementation, rescued heart development separately combination, obtaining complemented hearts...
Abstract Generation of upscaled quantities human‐induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CM), for therapeutic or testing applications, is both expensive and time‐consuming. Herein, a scalable bioprocess hiPSC‐CM expansion in stirred‐tank bioreactors (STB) developed. By combining the continuous activation Wnt pathway, through perfusion CHIR99021, within mild hypoxia environment, as aggregates maximized, reaching 4 billion pure 2L STB. In particular, importance i)...
ABSTRACT The bone marrow (BM) microenvironment plays a crucial role in regulating hematopoiesis, yet the molecular and functional changes associated with aging humans remain poorly understood. Using single-cell RNA sequencing (scRNA-seq), we uncovered transcriptional shifts BM endothelial cells (EC) mesenchymal stem (MSC) during aging. Our analysis revealed that aged sinusoidal EC adopt prothrombotic, exhibit mitochondrial dysfunction, have compromised vascular function. Additionally,...
Understanding the mechanisms that drive chimeric antigen receptor (CAR) T cell function and persistence in multiple myeloma (MM) remains a critical challenge for improving therapeutic outcomes. In this study, we applied single-cell multiomics gene regulatory network (GRN) analysis to characterize transcriptional dynamics clonal evolution of BCMA-targeted CAR cells longitudinally collected bone marrow (BM) peripheral blood (PB) samples from MM patients. Our results revealed infiltrating BM...
Abstract Because of the refractory nature mutant KRAS lung adenocarcinoma (LUAD) to current therapies, identification new molecular targets is essential. Genes with a prognostic role in LUAD have proven be potential for therapeutic development. Here we determine clinical, functional, and mechanistic inhibitor differentiation-1 (Id1) LUAD. Analysis cohorts from TCGA SPORE showed that high expression Id1 was marker poor survival patients harboring mutant, but not wild-type KRAS. Abrogation...
Genome-editing strategies, especially CRISPR-Cas9 systems, have substantially increased the efficiency of innovative therapeutic approaches for monogenic diseases such as primary hyperoxalurias (PHs). We previously demonstrated that inhibition glycolate oxidase using systems represents a promising option PH type I (PH1). Here, we extended our work evaluating efficacy liver-specific lactate dehydrogenase (LDH), key enzyme responsible converting glyoxylate to oxalate; this strategy would not...
Abstract Glial cells are key players in the initiation of innate immunity neurodegeneration. Upon damage, they switch their basal activation state and acquire new functions a context time-dependent manner. Since modulation neuroinflammation is becoming an interesting approach for treatment neurodegenerative diseases, it crucial to understand specific contribution these inflammatory reaction select experimental models that recapitulate what occurs human disease. Previously, we have...
Abstract Despite the development of novel therapies for acute myeloid leukemia, outcomes remain poor most patients, and therapeutic improvements are an urgent unmet need. Although treatment regimens promoting differentiation have succeeded in promyelocytic their role other leukemia subtypes needs to be explored. Here we identify characterize two lysine deacetylase inhibitors, CM-444 CM-1758, exhibiting capacity promote all at low non-cytotoxic doses, unlike commercial histone inhibitors....