A5066062442- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Nitric Oxide and Endothelin Effects
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bacterial Infections and Vaccines
- Microbial Metabolic Engineering and Bioproduction
- Legionella and Acanthamoeba research
- Adenosine and Purinergic Signaling
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Vitamin D Research Studies
- Bone health and osteoporosis research
- Advanced Electron Microscopy Techniques and Applications
- Vitamin K Research Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Glycosylation and Glycoproteins Research
University of Exeter
2022-2024
University of Nottingham
2019
Queen's Medical Centre
2019
University of Sheffield
2012-2014
Abstract Amongst the major types of archaeal filaments, several have been shown to closely resemble bacterial homologues Type IV pili (T4P). Within Sulfolobales , member species encode for three T4P, namely archaellum, UV-inducible pilus system (Ups) and adhesive (Aap). Whereas archaellum functions primarily in swimming motility, Ups UV-induced cell aggregation DNA-exchange, Aap plays an important role adhesion twitching motility. Here, we present a cryoEM structure model organism Sulfolobus...
Background Observational data associate lower levels of serum vitamin D with coronary artery calcification, cardiovascular events and mortality. However, there is little interventional evidence demonstrating that moderate deficiency plays a causative role in disease. This study examined the effects dietary receptor agonist (paricalcitol) administration apolipoprotein E knockout mice. Methods Mice were fed atherogenic diets normal content (1.5IU/kg) or without D. Paricalcitol, matched...
Pili are filamentous surface extensions that play roles in bacterial and archaeal cellular processes such as adhesion, biofilm formation, motility, cell-cell communication, DNA uptake horizontal gene transfer. The model archaeaon Sulfolobus acidocaldarius assembles three filaments of the type-IV pilus superfamily (archaella, adhesion pili UV-inducible pili), well a so-far uncharacterised fourth filament, named "thread". Here, we report on cryo-EM structure thread. filament is highly...
Enzyme-mediated synthesis of pharmaceutical compounds is a 'green' alternative to traditional synthetic chemistry, and microbial engineering opens up the possibility using whole cells as mini-factories. Whole-cell biocatalysis reduces cost by eliminating expensive enzyme purification cofactor addition steps, well resulting in increased stability. Haloferax volcanii model halophilic archaeon encoding highly salt organic solvent tolerant enzymes such alcohol dehydrogenase (HvADH2), which...
Abstract Amongst the major archaeal filament types, several have been shown to closely resemble bacterial homologues of Type IV pili (T4P). Within Sulfolobales, member species encode for three types T4P, namely archaellum, UV-inducible pilus (Uvp) and adhesive (Aap). Whereas archaellum functions primarily in swimming motility, Uvp UV-induced cell aggregation DNA-exchange, Aap plays an important role adhesion twitching motility. All previously solved appear almost identical helical...
<title>Abstract</title> Archaea of the order Thermoplasmatales push boundaries our current knowledge prokaryotic life. They show distinct cellular plasticity, heterogenous cell morphologies and lack a paracrystalline S-layer. As S-layer has previously been implicated in acting as stator scaffold for filaments driving propulsion, particularly archaella, we asked whether absence an precludes formation functional archaella or pili Thermoplasmatales. Using cryoEM, investigated two species...
Abstract Pili are ubiquitous filamentous surface extensions that play crucial roles for bacterial and archaeal cellular processes such as adhesion, biofilm formation, motility, cell-cell communication, DNA uptake horizontal gene transfer to name a few. Here we report on the discovery structure of thread – remarkably stable pilus belongs so-far largely unknown class protein filaments. We find filament is highly glycosylated interconnected via donor strand complementation, well isopeptide...
<title>Abstract</title> Amongst the major archaeal filament types, several have been shown to closely resemble bacterial homologues of Type IV pili (T4P). Within Sulfolobales, member species encode for three types T4P, namely archaellum, UV-inducible pilus (Uvp) and adhesive (Aap). Whereas archaellum functions primarily in swimming motility, Uvp UV-induced cell aggregation DNA-exchange, Aap plays an important role adhesion twitching motility. All previously solved appear almost identical...